Effects of extracellular matrix components on the differentiation of chick embryo tail bud mesenchyme in culture

Griffith, C.M.; Sanders, Esmond J.

doi:10.1111/j.1432-0436.1991.tb00223.x

Cited by 25 publications

(12 citation statements)

References 32 publications

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“…In addition, however, kidney tubules were observed in the coelomic grafts. We have recently found that isolated chick tail bud mesenchyme, under appropriate conditions in culture, can give rise to a variety of cell types comparable to those seen in situ (Griffith and Sanders 1991). These include myocytes, chondrocytes, neuroepithelium and neural crest derivatives (such as melanocytes), the differentiation of which appears to be influenced by substratum adhesiveness and the extracellular matrix (see below).…”

Section: Histogenetic Potential Of the Tail Budmentioning

confidence: 99%

“…In the early development of the chick central nervous system, the form of N-CAM with a low PSA content is believed to maintain the integrity of the tissue under conditions of mechanical stress, e.g. folding Griffith and Sanders 1991) of the neural plate to form primary neural tube (Sunshine et al 1987). This is achieved by increasing cell-cell contact through homophilic binding of N -C A M molecules on one cell to those on a neighbouring cell, thus facilitating any further interactions between the cells (Rutishauser et al 1988).…”

Section: Sialoconjugates N-cam and Tail Bud Developmentmentioning

confidence: 99%

“…As the extracellular matrix has been known to maintain the differentiated state of various epithelia (Beck et al 1990), we tested the ability of its various components to support the mesenchymal to epithelial transformation of tail bud mesenchymal cells into neuroepithelium in culture (Griffith and Sanders 1991). Several of the matrix components tested were found to favour the differentiation of cell types distinct from the morphologically homogeneous mass of explanted tail bud mesenchymal cells.…”

Section: Effects Of Extracellular Matrix Componentsmentioning

confidence: 99%

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The vertebrate tail bud: three germ layers from one tissue

1992

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The tail bud of amniote embryos comprises a mass of apparently undifferentiated mesenchymal cells located at the caudal limit of the embryo, representing the remains of Hensen's node and the primitive streak. These cells have the potential to give rise to a variety of different tissues including the posterior or 'secondary' neural tube, the tail gut, and somites and their derivatives. This seemingly homogeneous accumulation of cells therefore has the capacity to differentiate into tissues which in more cranial regions of the embryo are derived from cells of different germ layers. In this review, the tissue contributions of the tail bud in various vertebrate classes are discussed, with particular attention to the mesenchymal-to-epithelial transformation that characterizes the process of secondary neurulation, and which distinguishes it from the epithelial folding that occurs during primary neurulation in more cranial regions. Recent studies suggest that the transformation is accompanied by extensive changes in the cell surface oligosaccharide complement of the differentiating cells, and that the sialyted form of N-CAM is expressed both temporally and spatially in a manner that suggests a role for it in the process. The pluripotential nature of the tail bud mesenchyme may be revealed experimentally by grafting the tissue ectopically, or by culturing it on different substrata. In the latter case, the mesenchyme can be demonstrated to give rise to myocytes, chondrocytes, neuroepithelium and neural crest derivatives such as melanocytes, depending on the nature of the culture substratum. It is concluded that the tail bud mesenchyme represents a developing system which is readily amenable to experimentation and should provide insights into the general mechanisms of cell differentiation and transformation.

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Section: Histogenetic Potential Of the Tail Budmentioning

confidence: 99%

Section: Sialoconjugates N-cam and Tail Bud Developmentmentioning

confidence: 99%

Section: Effects Of Extracellular Matrix Componentsmentioning

confidence: 99%

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The vertebrate tail bud: three germ layers from one tissue

1992

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“…Chondrogenesis, one of the earliest morphogenetic events for cartilage development in the limb, comprises a highly coordinated series of events involving proliferation, condensation, and chondrogenic differentiation of mesenchymal cells to produce the bones of the limb. This process is orchestrated by many factors that include secreted factors, such as fibroblast growth factor, transforming growth factor-b, hedgehog and Wnt families of proteins, and cell-cell and cell-matrix adhesion proteins, such as N-cadherin, N-CAM, integrins and extracellular matrix proteins like fibronectin and collagen (Griffith and Sanders, 1991;ChimalMonroy and Díaz de Leó n, 1999).…”

mentioning

confidence: 96%

Alterations in the temporal expression and function of cadherin‐7 inhibit cell migration and condensation during chondrogenesis of chick limb mesenchymal cells in vitro

Kim

Kang

Jin

2009

Journal Cellular Physiology

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Endochondral bone formation requires a complex interplay among immature mesenchymal progenitor cells to form the cartilaginous anlagen, which involves migration, aggregation and condensation. Even though condensation of chondrogenic progenitors is an essential step in this process, its mechanism(s) has not been well studied. Here, we show that cadherin-7 plays a central role in cellular condensation by modulating cell motility and migration. In this study, many mesenchymal cells failed to migrate, and precartilage condensation was inhibited, after knockdown of endogenous cadherin-7 levels. Exposure of mesenchymal cells to SB203580 (a specific inhibitor of p38MAPK), LiCl (an inhibitor of GSK-3beta) or overexpression of beta-catenin resulted in inhibition of cadherin-7 levels and, subsequently, suppression of cell migration. Collectively, our results suggest that cadherin-7 controls cell migration in chick limb bud mesenchymal cells, and that p38MAPK and GSK signals are responsible for regulating cadherin-7-mediated cell migration.

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“…The filum terminale or conus is replaced by the lipomatous mass. Reports that the cells of the caudal eminence are multipotent and that changes in the environment of these cells can lead to various directions of differentiation support the latter theory [12, 13]. …”

Section: Discussionmentioning

confidence: 76%

Pathogenesis of Lumbosacral Lipoma: A Test of the ‘Premature Dysjunction’ Theory

Shin

Cho

et al. 2001

Pediatr Neurosurg

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Premature separation of the neuroectoderm from the ipsilateral surface ectoderm allowing mesenchymal tissue to invade into the central canal, or ‘premature dysjunction’ theory, was proposed for the pathogenesis of dorsal type lumbosacral lipoma. To test this theory, the unilateral neural fold was incised using Hamburger and Hamilton stage 12 or 13 chick embryos. Among 35 embryos evaluated, 15 showed abnormal findings, and of these one showed findings which suggested lumbosacral lipoma: a back lump, blending of the neuroepithelium and mesenchyme through indistinct basement membrane and vertebral body abnormalities. The other 14 embryos showed abnormalities including blunt tails, open neural tube defects, incomplete closure of the dorsal neuroepithelium with intact skin, skin dimples, disorganized gray matter, scoliosis, ectopic neuroepithelium and an accessory spinal cord. The results revealed that the incision of the unilateral neural fold in the early chick embryo may produce a lesion suggestive of lumbosacral lipoma, a finding which supports the premature dysjunction theory. This method needs further refinement to overcome technical difficulties, high mortality, and a low yield before being adopted as an experimental model for lumbosacral lipoma.

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Effects of extracellular matrix components on the differentiation of chick embryo tail bud mesenchyme in culture

Cited by 25 publications

References 32 publications

The vertebrate tail bud: three germ layers from one tissue

The vertebrate tail bud: three germ layers from one tissue

Alterations in the temporal expression and function of cadherin‐7 inhibit cell migration and condensation during chondrogenesis of chick limb mesenchymal cells in vitro

Pathogenesis of Lumbosacral Lipoma: A Test of the ‘Premature Dysjunction’ Theory

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