Alterations in the temporal expression and function of cadherin‐7 inhibit cell migration and condensation during chondrogenesis of chick limb mesenchymal cells in vitro

Kim, Dongkyun; Kang, Shin‐Sung; Jin, Eun‐Jung

doi:10.1002/jcp.21840

Cited by 10 publications

(7 citation statements)

References 50 publications

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“…Many different studies have emphasized the potential importance of different adhesion molecules and cytoskeletal regulators in chondrogenic differentiation [4], [12], [17]–[20]. However, their precise role in the chondrogenic signaling cascade is obscure and mice with targeted deficiencies of the most relevant adhesion molecules show a normal pattern of appendicular skeleton [11], [13], [21].…”

Section: Resultsmentioning

confidence: 99%

Defining the Earliest Transcriptional Steps of Chondrogenic Progenitor Specification during the Formation of the Digits in the Embryonic Limb

et al. 2011

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The characterization of genes involved in the formation of cartilage is of key importance to improve cell-based cartilage regenerative therapies. Here, we have developed a suitable experimental model to identify precocious chondrogenic events in vivo by inducing an ectopic digit in the developing embryo. In this model, only 12 hr after the implantation of a Tgfβ bead, in the absence of increased cell proliferation, cartilage forms in undifferentiated interdigital mesoderm and in the course of development, becomes a structurally and morphologically normal digit. Systematic quantitative PCR expression analysis, together with other experimental approaches allowed us to establish 3 successive periods preceding the formation of cartilage. The “pre-condensation stage”, occurring within the first 3 hr of treatment, is characterized by the activation of connective tissue identity transcriptional factors (such as Sox9 and Scleraxis) and secreted factors (such as Activin A and the matricellular proteins CCN-1 and CCN-2) and the downregulation of the galectin CG-8. Next, the “condensation stage” is characterized by intense activation of Smad 1/5/8 BMP-signaling and increased expression of extracellular matrix components. During this period, the CCN matricellular proteins promote the expression of extracellular matrix and cell adhesion components. The third period, designated the “pre-cartilage period”, precedes the formation of molecularly identifiable cartilage by 2–3 hr and is characterized by the intensification of Sox 9 gene expression, along with the stimulation of other pro-chondrogenic transcription factors, such as HifIa. In summary, this work establishes a temporal hierarchy in the regulation of pro-chondrogenic genes preceding cartilage differentiation and provides new insights into the relative roles of secreted factors and cytoskeletal regulators that direct the first steps of this process in vivo.

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Section: Resultsmentioning

confidence: 99%

Defining the Earliest Transcriptional Steps of Chondrogenic Progenitor Specification during the Formation of the Digits in the Embryonic Limb

et al. 2011

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“…122 Finally, cadherin-7 has been shown to be necessary for cartilage condensation in HH22-23 chick limb mesenchymal cells. 74 Knockdown of cadherin-7 resulted in impaired cell migration and failed precursor condensation, while overexpression of β-catenin also resulted in inhibition of cadherin-7 and suppression of cell migration. 74 Thus, in addition to N-cadherin, cadherin-7 may control cellular condensation during chondrogenesis.…”

Section: Cadherin Regulation Of Chondrogenesis and Osteogenesismentioning

confidence: 99%

Cell-cell junctions in developing and adult tendons

2019

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Tendons connect muscles to bones to transfer the forces necessary for movement. Cell-cell junction proteins, cadherins and connexins, may play a role in tendon development and injury. In this review, we begin by highlighting current understanding of how cell-cell junctions may regulate embryonic tendon development and differentiation. We then examine cell-cell junctions in postnatal tendon, before summarizing the role of cadherins and connexins in adult tendons. More information exists regarding the role of cell-cell junctions in the formation and homeostasis of other musculoskeletal tissues, namely cartilage and bone. Therefore, to inform future tendon studies, we include a brief survey of cadherins and connexins in chondrogenesis and osteogenesis, and summarize how cell-cell junctions are involved in some musculoskeletal tissue pathologies. An enhanced understanding of how cell-cell junctions participate in tendon development, maintenance, and disease will benefit future regenerative strategies.

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“…1). Cadherin-11 is involved in cell sorting of the limb mesenchyme in vitro (Kimura et al, 1995), and cadherin-dependent cell adhesion regulates chondrogenesis of the limb mesenchyme (Kim et al, 2009). The polarized distribution of cadherin proteins in the limb bud may affect position-dependent chondrogenesis and cartilage morphogenesis thorough modification of cell adhesiveness.…”

Section: Cell Surface Moleculesmentioning

confidence: 99%

Spatiotemporal changes in cell adhesiveness during vertebrate limb morphogenesis

Wada

2011

Developmental Dynamics

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During vertebrate limb development, various molecules are expressed in the presumptive limb field or the limb bud in a spatiotemporal-specific manner. The combination of these molecules regulates cellular properties that affect limb initiation and its morphogenesis, especially cartilage formation. Cell adhesiveness of the limb mesenchyme is a key factor in the regulation of cell distribution. Differential adhesiveness of mesenchymal cells is first observed between cells in the presumptive limb field and flank region, and the adhesiveness of the cells in the limb field is higher than that of cells in the flank region. In the limb bud, the adhesiveness of mesenchymal cells shows spatiotemporal difference, which reflects the positional identity of the cells. Position-dependent cell adhesiveness is also observed in blastema cells of the regenerating limb. Therefore, local changes in cell adhesiveness are observed during limb development and regeneration, suggesting significant roles for cell adhesiveness in limb morphogenesis. Developmental Dynamics 240:969-978,

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Alterations in the temporal expression and function of cadherin‐7 inhibit cell migration and condensation during chondrogenesis of chick limb mesenchymal cells in vitro

Cited by 10 publications

References 50 publications

Defining the Earliest Transcriptional Steps of Chondrogenic Progenitor Specification during the Formation of the Digits in the Embryonic Limb

Defining the Earliest Transcriptional Steps of Chondrogenic Progenitor Specification during the Formation of the Digits in the Embryonic Limb

Cell-cell junctions in developing and adult tendons

Spatiotemporal changes in cell adhesiveness during vertebrate limb morphogenesis

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