Extracellular matrix/stromal vascular fraction gel conditioned medium accelerates wound healing in a murine model

Deng, Chao; He, Yunfan; Feng, Jiaxuan; Dong, Ziqing; Yao, Yao; Mok, Hsiaopei; Lin, Maohui; Lu, Feng

doi:10.1111/wrr.12602

Cited by 26 publications

(19 citation statements)

References 56 publications

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“…For instance, ECM has been shown to play an important role in the regulation of stem cells and different ECM in ex vivo culture system can result in the differential expression of genes [23]. For example, 3D Matrigel has been shown to support the stemness of LNC better than coated Matrigel [3], and the conditioned medium containing ECM components promoted wound healing of mice skin [24]. Some ECM components that may plan a vital role include EGFL6 and FBLN1.…”

Section: Discussionmentioning

confidence: 99%

Differential Gene Expression between Limbal Niche Progenitors and Bone Marrow Derived Mesenchymal Stem Cells

Wang¹,

Shen²,

Xu³

et al. 2020

Int. J. Med. Sci.

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Purpose: To compare the difference in gene expression between human limbal niche cells (LNC) and bone marrow derived mesenchymal stem cells (BMMSC). Methods: LNC were isolated by collagenase and expanded in modified embryonic stem cell medium (MESCM) on a Matrigel coated plastic plate. Cell diameters were measured with Image J software. Relative gene expression levels between LNC and BMMSC were compared using Affymetrix Human Primer View Gene Expression Array. A subset of differentially expressed genes was verified by RT-qPCR. The protein level of LAMA1 and COL4A1 was confirmed by Western blot and immunostaining. Results: The average diameter of LNC was 10.2±2.4 μm, which was significantly smaller than that of BMMSC (14 ±3.4 μm) (p<0.0001). Expression of 20,432 genes was examined by Gene Expression Array, among which expression of 349 genes in LNC was 10-fold or higher than that of BMMSC and expression of 8 genes in LNC was 100-fold or higher than that of BMMSC, while expression of 3 genes in BMMSC was 100-fold higher than that of LNC. GO analysis and pathway analysis showed that the differentially expressed genes were mainly enriched in the extracellular matrix receptor interaction pathway and Wnt signaling pathway. In addition, RT-qPCR results demonstrated that the expression of CD73, CD90, CD105, PDGFRβ, Vimentin, SCF, KIT (CD117), COL14A1, LAMA2, THBS2, FZD1, BMP2 and CXCL12 genes in LNC were at least 2 folds higher than BMMSC. The protein level of LAMA1 was higher but the protein level of COL4A1 was lower in LNC than that in BMMSC. Conclusion: LNC exhibit differential gene expression from BMMSC in the extracellular matrix (ECM) receptor interaction pathway and Wnt signaling pathway, suggesting that LNC have their unique signaling pathways to support limbal stem cell niches.

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Section: Discussionmentioning

confidence: 99%

Differential Gene Expression between Limbal Niche Progenitors and Bone Marrow Derived Mesenchymal Stem Cells

Wang¹,

Shen²,

Xu³

et al. 2020

Int. J. Med. Sci.

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“…They recently demonstrated that ECM/SVF-gel exhibited greater effects on wound healing compared with SVF suspension, mainly as a result of the high concentration of growth factors secreted by the ECM/SVF-gel [20]. Furthermore, CM from ECM/SVF-gel was rich in growth factors and improved wound healing compared with conventional SVF-CM [21].…”

Section: Introductionmentioning

confidence: 99%

Promotion of Hair Growth by Conditioned Medium from Extracellular Matrix/Stromal Vascular Fraction Gel in C57BL/6 Mice

Xiao

Deng

et al. 2020

Stem Cells International

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Adipose-derived stem cell- (ADSC-) based regenerative medicine has expanded to include the treatment of hair loss. However, stem cell therapy remains a relatively recent technique, and reports of its use for treating alopecia are rare. ADSCs exert biological functions via the paracrine actions of various growth factors and cytokines. Conditioned medium from ADSCs (ADSCs-CM) is a cell-free suspension rich in growth factors and cytokines that has demonstrated a significant role in stimulating hair growth, with encouraging outcomes in terms of hair regeneration and hair growth. Extracellular matrix/stromal vascular fraction gel (ECM/SVF-gel) is an ADSC- and adipose native extracellular matrix-enriched product for cytotherapy. In this study, we compared the effects of CM from ECM/SVF-gel (ECM/SVF-CM) and from stem cells (SVF-CM) on hair growth in mice. ECM/SVF-CM stimulated hair growth more than SVF-CM, through promoting the proliferation of dermal papilla cells and cells in the bulge, neovascularization, and anagen induction. ECM/SVF-CM might, thus, provide an effective and improved strategy for promoting hair growth. These data provide a theoretical foundation for the clinical administration of ECM/SVF-CM for the treatment of hair loss.

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“…Fat-derived products vary between conventional CF, CAL, and mechanically processed fat grafts such as SVF gel. Because CF has been extensively applied, many fat-derived products have been developed to condense ASCs for not only improved volume retention, but also for improved therapeutic e cacy in wound healing and skin diseases [27,31]. CAL attaches ASCs to the aspirated fat, converting ASC-poor aspirated fat to ASC-rich fat [32].…”

Section: Discussionmentioning

confidence: 99%

Mechanical micronization of lipoaspirates for treatment of bleomycin-induced scleroderma in nude mice

Jiang

Wang

Lin

et al. 2020

Preprint

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Background Scleroderma is a chronic autoimmune disease that causes hardening of the skin. Adipose tissue is used as a regenerative treatment for scleroderma, and mechanical micronization of adipose tissue can concentrate stem cells, improving its therapeutic efficacy. Stromal vascular fraction gel (SVF gel) is produced by subjecting lipoaspirates to a series of mechanical processes. The present study aimed to assess the therapeutic effect of SVF gel on dermal scleroderma. Methods Scleroderma was induced in nude mice by daily subcutaneous injections of bleomycin (BLM) for 4 weeks. SVF gel, cell-enriched lipoaspirates (CAL), or Coleman fat (CF) (0.1 mL) was injected subcutaneously into local sclerosis lesions. Histological analyses, TUNEL assay, and qRT-PCR were conducted 4 weeks post-transplantation. Results SVF gel, CAL, and CV decreased dermal thickness in the scleroderma model (P < 0.05), and sclerosis was significantly decreased in the SVF gel and CAL groups. SVF gel and CAL decreased macrophage infiltration, pro-inflammatory cytokine levels, and fibrosis in transferred fat and/or skin lesions (P < 0.05). This revealed anti-inflammatory and anti-fibrotic effects of adipose-derived stem cells (ASCs) therapy in scleroderma (P < 0.05). SVF gel exhibited the most potent anti-inflammatory and anti-fibrotic effects among treatment groups (P < 0.05). Conclusion SVF gel and CAL significantly reversed dermal sclerosis, and were more effective than CF. The SVF gel group exhibited the lowest inflammation both in transferred fat and in skin lesions, and also the lowest fibrosis among the three groups. This study suggested that SVF gel was a more suitable adipose-based therapy for topical treatment of scleroderma patients.

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Extracellular matrix/stromal vascular fraction gel conditioned medium accelerates wound healing in a murine model

Cited by 26 publications

References 56 publications

Differential Gene Expression between Limbal Niche Progenitors and Bone Marrow Derived Mesenchymal Stem Cells

Differential Gene Expression between Limbal Niche Progenitors and Bone Marrow Derived Mesenchymal Stem Cells

Promotion of Hair Growth by Conditioned Medium from Extracellular Matrix/Stromal Vascular Fraction Gel in C57BL/6 Mice

Mechanical micronization of lipoaspirates for treatment of bleomycin-induced scleroderma in nude mice

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