Promotion of Hair Growth by Conditioned Medium from Extracellular Matrix/Stromal Vascular Fraction Gel in C57BL/6 Mice

Abstract

Adipose-derived stem cell- (ADSC-) based regenerative medicine has expanded to include the treatment of hair loss. However, stem cell therapy remains a relatively recent technique, and reports of its use for treating alopecia are rare. ADSCs exert biological functions via the paracrine actions of various growth factors and cytokines. Conditioned medium from ADSCs (ADSCs-CM) is a cell-free suspension rich in growth factors and cytokines that has demonstrated a significant role in stimulating hair growth, with encouraging outcomes in terms of hair regeneration and hair growth. Extracellular matrix/stromal vascular fraction gel (ECM/SVF-gel) is an ADSC- and adipose native extracellular matrix-enriched product for cytotherapy. In this study, we compared the effects of CM from ECM/SVF-gel (ECM/SVF-CM) and from stem cells (SVF-CM) on hair growth in mice. ECM/SVF-CM stimulated hair growth more than SVF-CM, through promoting the proliferation of dermal papilla cells and cells in the bulge, neovascularization, and anagen induction. ECM/SVF-CM might, thus, provide an effective and improved strategy for promoting hair growth. These data provide a theoretical foundation for the clinical administration of ECM/SVF-CM for the treatment of hair loss.

Vitamin A alleviates heat stress‐induced damage to hair follicle development in Rex rabbits

“…5 Hepatocyte growth factor (HGF), epidermal growth factor (EGF), and insulin-like growth factor 1 (IGF1) positively regulate hair follicle development. 6 Bone morphogenetic proteins (BMPs) can inhibit hair follicle development. 7 The growth and development of hair follicles are also regulated by many signal pathways.…”

“…Hair follicles undergo self‐renewal and periodic growth, and these processes are regulated by many factors 5 . Hepatocyte growth factor (HGF), epidermal growth factor (EGF), and insulin‐like growth factor 1 (IGF1) positively regulate hair follicle development 6 . Bone morphogenetic proteins (BMPs) can inhibit hair follicle development 7 .…”

Adipose-derived stem cells applied in skin diseases, wound healing and skin defects: a review

“…A combination of ASCs and PRP showed an even better effect. Xiao et al [150] compared CM from an ECM/SVF gel with SVF-CM in a mouse model. Three weekly applications of ECM/SVF-CM stimulated hair growth more than SVF-CM by promoting the proliferation of dermal papilla and bulge cells as well as neovascularization and anagen induction.…”

Mesenchymal Stromal Cell-Conditioned Medium for Skin Diseases: A Systematic Review

“…Ten studies evaluated the effect of MSC-CM on hair growth at the preclinical level (Park et al, 2010;Won et al, 2010Won et al, , 2015Dong et al, 2014;Shim, 2015;Choi et al, 2019;Gunawardena et al, 2019a;Ou et al, 2020;Xiao et al, 2020; Table 7). MSCs were isolated mainly from human hair follicles (Won et al, 2010;Won et al, 2015;Gunawardena et al, 2019a;Ou et al, 2020) (40%, 4/10) and human adipose tissue (Park et al, 2010;Choi et al, 2019;Xiao et al, 2020) (30%, 3/10). Other tissues used were human amniotic fluid , human deciduous teeth (Gunawardena et al, 2019a), human dermal cells (Shim, 2015), and murine bone marrow (Dong et al, 2014).…”

“…All studies employed murine models. Mesenchymal stromal cell-CM was used subcutaneously (Park et al, 2010;Won et al, 2015;Choi et al, 2019;Gunawardena et al, 2019a;Xiao et al, 2020) (50%, 5/10) or intradermally (Dong et al, 2014) injected or topically applied (Won et al, 2010;Shim, 2015;Ou et al, 2020) (40%, 4/10). MSC-CM was tested on 86 animals with shaved regions.…”