Extracellular Matrix of Current Biological Scaffolds Promotes the Differentiation Potential of Mesenchymal Stem Cells

Voß, Andreas; McCarthy, Mary Beth; Hoberman, Alexander; Cote, Mark P.; Imhoff, Andreas B.; Mazzocca, Augustus D.; Beitzel, Knut

doi:10.1016/j.arthro.2016.04.033

Cited by 19 publications

(12 citation statements)

References 59 publications

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“…In recent studies, this process was not successful because of immature and non-functional IPCs production. The fibronectin (as a common component of extracellular matrix) is used for enhancing pluripotent and adult stem cell differentiation (Lumelsky et al, 2001;Wei et al, 2013;Voss et al, 2016). As a result, the methods should be safer and less expensive.…”

Section: Discussionmentioning

confidence: 99%

“…The VEGF-A is demonstrated as highly produced in islets compared to other pancreatic cells, and also, the VEGF-A plays an important role in the formation of islet structure (WataDa, 2010). The fibronectin (as a common component of extracellular matrix) is used for enhancing pluripotent and adult stem cell differentiation (Lumelsky et al, 2001;Wei et al, 2013;Voss et al, 2016). Thus, the PRP was utilized to induce iPSCs toward pancreatic differentiation and the generation of IPCs.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Generation of Insulin‐Producing Cells From Human‐Induced Pluripotent Stem Cells Using a Stepwise Differentiation Protocol Optimized With Platelet‐Rich Plasma

Enderami

Mortazavi

Soleimani

et al. 2017

Journal Cellular Physiology

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Studies on patient-specific human-induced pluripotent stem cells (hiPSCs) as well as a series of autologous growth factors presumably will reveal their many benefits for cell base replacement therapy in type 1 diabetes mellitus (TIDM) patients in the future. For this purpose, we established a multistep protocol by adding platelet-rich plasma (PRP) that induce the hiPSCs into insulin-producing cells (IPCs). We present here a differentiation protocol consisting of five stages in two groups including protocol with PRP and without PRP. Charac-teristics of derived IPCs in both groups were evaluated at the mRNA and protein levels, cell cycle and viability in the end stage of cell differentiation. The in vitro studies indicated the treatment of hiPSCs in the protocol with PRP resulting in differentiated cells with strong characteristics of IPCs including islet-like cells, the expression of mature and functional pancreatic beta cell specific marker genes. In addition to these pancreatic specific markers were detected by immunochemistry and Western blot. Our differentiated cells in two groups secreted insulin and C-peptide in a glucose stimulation test by ELISA showing in vitro functional. The results of the cell cycle assay confirmed that differentiation has been done. We reported for the first time that PRP might be ideal additive in the culture medium to induce pancreatic differentiation in the hiPSCs. This study provides a new approach to investigate the role of PRP in pancreatic differentiation protocols and enhance the feasibility of using patient-specific iPSCs and autologous PRP for future beta cells replacement therapies for T1DM. J. Cell. Physiol. 232: 2878-2886, 2017. © 2016 Wiley Periodicals, Inc.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Generation of Insulin‐Producing Cells From Human‐Induced Pluripotent Stem Cells Using a Stepwise Differentiation Protocol Optimized With Platelet‐Rich Plasma

Enderami

Mortazavi

Soleimani

et al. 2017

Journal Cellular Physiology

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show abstract

“…The cBMA was plated in 100 mm 2 Primaria culture dishes (Fisher Scientific, Waltham, MA) and cultured according to a standard protocol. 27 Cells were placed an incubator at 37°C with 5% CO 2 . Media was changed after 24 hours and plates were checked every day for the appearance of colonies and contamination.…”

Section: Methodsmentioning

confidence: 99%

Significant Improvement in Shoulder Function and Pain in Patients Following Biologic Augmentation of Revision Arthroscopic Rotator Cuff Repair Using an Autologous Fibrin Scaffold and Bone Marrow Aspirate Derived From the Proximal Humerus

Voß

McCarthy

Bellas

et al. 2021

Arthroscopy, Sports Medicine, and Rehabilitation

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Purpose To clinically evaluate patients who underwent a biologic augmentation technique in revision arthroscopic rotator cuff repair using an autologous fibrin scaffold and concentrated stem cells isolated from bone marrow aspirate (BMA) obtained from the proximal humerus. Methods This is a retrospective review of prospectively collected data from patients who underwent biologic augmentation of revision arthroscopic rotator cuff repair using an autologous fibrin scaffold and BMA obtained from the proximal humerus between 2014 and 2015. Minimum follow-up was 12 months. Outcome measures were collected preoperatively and postoperatively including range of motion as well as American Shoulder and Elbow Surgeons Shoulder Form, Simple Shoulder Test, single assessment numeric evaluation, and visual analog score. In addition, BMA samples of each patient were assessed for the number of nucleated cells and colony-forming units. Regression analysis was performed to investigate whether the number of nucleated cells and colony-forming units had an influence on outcome and failure. Results Ten patients who underwent biologic augmentation of revision arthroscopic rotator cuff repair using an autologous fibrin scaffold and concentrated BMA obtained from the proximal humerus between 2014 and 2015 were included. The mean follow-up time was 30.7 (range: 12-49) months. Four patients were revised at final follow-up. Postoperative clinical scores improved significantly: American Shoulder and Elbow Surgeons (28.1 ± 5.4 to 60.9 ± 9.0; P < .01), single assessment numeric evaluation (6.6 ± 2.3 to 65.1 ± 10.9; P < .01), visual analog scale (7.2 ± 0.9 to 3.1 ± 0.9; P < .01), and Simple Shoulder Test (1.6 ± 0.5 to 10.3 ± 5.7; P < .01). Postoperative range of motion increased significantly with regard to flexion (97.0 ± 13.6 to 151.0 ± 12.2; P < .01) and abduction (88.0 ± 14.0 to 134.0 ± 15.1; P = .038) but not with external rotation (38.0 ± 5.7 to 50.5 ± 6.5; P = .16). Less pain was correlated to an increased number of nucleated cells ( P = .026); however, there was no correlation between failure rate and number of nucleated cells ( P = .430). Conclusions Patients who underwent biologic augmentation of revision arthroscopic rotator cuff repair using an autologous fibrin scaffold and concentrated BMA demonstrated a significant improvement in shoulder function along with reduction of pain. However, the overall revision rate for this procedure was 40%. Level of Evidence Level IV, therapeutic case series.

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“…Extracellular matrices have been recently developed as a scaffold patch to support both cell attachment and matrix formation, aiding in tendon healing after rotator cuff repair [116]. These patches have been theorized to help augment repairs either by acting as a load-sharing device that reduces strain across the repair site or by acting as a scaffold to support cell attachment, matrix synthesis, and new tissue formation [117]. Data on the efficacy of this augment is limited but promising.…”

Section: Scaffolding Devicesmentioning

confidence: 99%

Review of Ortho-Biologics in Rotator Cuff Repair

Konopitski¹,

Malige²

2022

Shoulder Surgery for RC Pathology, Arthropathy and Tumors

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Rotator cuff repair is one of the most commonly performed surgeries in orthopedics, yet rates of postoperative failure and retear remain relatively high. Poor biology and limited healing potential at the cuff insertion are frequently cited as potential confounders to otherwise technically successful surgeries. Over the past several years, ortho-biologics have been developed in an attempt to augment rotator cuff repairs. The following review will briefly cover normal biomechanics and histology of the rotator cuff and how this is altered in cuff tears, provide an in-depth summary of the available literature on various ortho-biologic agents, outline the limitations of each agent and give an idea on the future of ortho-biologics in rotator cuff.

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Extracellular Matrix of Current Biological Scaffolds Promotes the Differentiation Potential of Mesenchymal Stem Cells

Cited by 19 publications

References 59 publications

Generation of Insulin‐Producing Cells From Human‐Induced Pluripotent Stem Cells Using a Stepwise Differentiation Protocol Optimized With Platelet‐Rich Plasma

Generation of Insulin‐Producing Cells From Human‐Induced Pluripotent Stem Cells Using a Stepwise Differentiation Protocol Optimized With Platelet‐Rich Plasma

Significant Improvement in Shoulder Function and Pain in Patients Following Biologic Augmentation of Revision Arthroscopic Rotator Cuff Repair Using an Autologous Fibrin Scaffold and Bone Marrow Aspirate Derived From the Proximal Humerus

Review of Ortho-Biologics in Rotator Cuff Repair

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