Extracellular matrix metalloproteinase inducer (<scp>EMMPRIN</scp>) is a potential biomarker of angiogenesis in proliferative diabetic retinopathy

El‐Asrar, Ahmed M. Abu; Ahmad, Ajmal; Alam, Kaiser; Siddiquei, Mohammad Mairaj; Mohammad, Ghulam; Hertogh, Gert De; Mousa, Ahmed; Opdenakker, Ghislain

doi:10.1111/aos.13284

Cited by 34 publications

(39 citation statements)

References 38 publications

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“…Therefore, there are several anti-VEGF drugs under investigation for treating DR including macugen and avastin [32]. The previous studies reported that CD31 is an angiogenic marker protein that contributes to neovascularization in DR rats' retina [33]. In our study, the levels of VEGF in vitreous, retinas and serum from diabetic rats were apparently increased.…”

Section: Discussionsupporting

confidence: 51%

The protective effects of lycopus lucidus turcz in diabetic retinopathy and its possible mechanisms

Liu

Bhuvanagiri

2019

Artificial Cells, Nanomedicine, and Biotechnology

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The aim of the present study was to investigate the effect of Lycopus lucidus Turcz (LT) on diabetic retinopathy (DR) and its underlying mechanisms. SD rats and human retinal microvascular endothelial cells (HRECs) were applied for establishment DR model. HE and TUNEL staining were used to evaluate the pathological changes and apoptosis of retinal ganglion cells. Additionally, retinal vessels were detected by immunofluorescence staining with CD31 and VEGF. The function of BRB was observed using Evans blue. Moreover, the oxidative stress, inflammation and angiogenesis associated factors were measured respectively. The expression of p38-MAPK/NF-jB signalling proteins were detected by Western blot. The results demonstrated that pathological changes and retinal optic disc cells apoptosis in retinas of diabetic rats, both of which were reduced in the LT-treated group. And LT treatment attenuated the levels of oxidative stress, inflammation and angiogenesis factors. Importantly, the expression levels of p-p38, pERK , p-JNK and NF-jB were decreased. After treatment with TNF-a combined with LT, the levels of inflammatory factors were decreased but higher than the negative control. Taken together, the results suggested that LT treatment is of therapeutic benefit by ameliorating oxidative stress, inflammation and angiogenesis of DR via p38-MAPK/NF-jB signaling pathway.

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Section: Discussionsupporting

confidence: 51%

The protective effects of lycopus lucidus turcz in diabetic retinopathy and its possible mechanisms

Liu

Bhuvanagiri

2019

Artificial Cells, Nanomedicine, and Biotechnology

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“…Neovascularization, a complex process for new blood vessels formation in which endothelial cells migration, adhesion and tubular formation play a very important role [29] , is a pathological hallmark feature for PDR [30] . Edu assay, Transwell cell migration assay and tube formation assay are mature methods to simulate in vitro neovascularization [31][32][33] .…”

Section: Discussionmentioning

confidence: 99%

Annexin A2 Promotes Development of Retinal Neovascularization through PI3K/ AKT Pathway

Zhao

Zhao³

2020

Preprint

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Background RNV is a pathological characteristic of PDR and ANXA2 play an important role in the process of RNV while the mechanism remains unclear. We explore the role and molecular basis of ANXA2 in the formation of RNV and seek for new potential targets for the prevention and treatment of PDR. Methods Lentivirus containing plasmids which can interfere ANXA2 and overexpress ANXA2 were packaged and infected HRECs, dividing HRECs into 4 groups. Moreover, 1ul SC79 solution was added in shA2 group and 1ul LY294002 solution was added into lentiA2 group. Western Blot was used to detect expression of ANXA2 and changes in phosphorylation degree of major proteins in PI3K/AKT signaling pathway in each group of HRECs. HRECs of all groups were used to perform EDu cell proliferation assay, Transwell cell migration assay and Matrix tube formation assay. C57BL/6J mice were randomly divided into 3 groups. Mice of OIR group were injected intraperitoneally with 0.05ml PBS every day from 7th to 10th day while mice of LY294002 treatment group were injected with 0.05ml LY294002 solution and no treatment in the control group. Lectin GS-IB4 fluorescence staining was used to observe RNV in mice in all groups. The expression of ANXA2 in mouse retinas was detected by Westen-blot. Results The proliferation, immigration and angiogenesis ability of HRECs is lower in shA2 group than shNC and SC79 treatment group while higher in lentiA2 group than lenti-EGFP and LY294002 treatment group. ANXA2 expression is significantly higher in retina of mice in OIR group and LY294002 treatment group. RNV is significantly less severe in LY294002 treatment group than that in OIR group. Conclusions ANXA2 can promote development of RNV through PI3K/ AKT Pathway.

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“…Several studies have confirmed an angiogenic function of MMP-14. It mediated corneal angiogenesis through VEGFR1 cleavage and was upregulated in proliferative diabetic retinopathy [132,133]. Another report showed that MMP-14 promotes invasiveness and angiogenesis through VEGF and PTTG [134].…”

Section: Pro-angiogenic Mirnas In Crcmentioning

confidence: 99%

Angioregulatory microRNAs in Colorectal Cancer

Soheilifar

Grusch

Neghab

et al. 2019

Cancers

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Colorectal cancer (CRC) is one of the leading causes of cancer mortality. Angiogenesis is a rate-determining step in CRC development and metastasis. The balance of angiogenic and antiangiogenic factors is crucial in this process. Angiogenesis-related genes can be regulated post-transcriptionally by microRNAs (miRNAs) and some miRNAs have been shown to shuttle between tumor cells and the tumor microenvironment (TME). MiRNAs have context-dependent actions and can promote or suppress angiogenesis dependent on the type of cancer. On the one hand, miRNAs downregulate anti-angiogenic targets and lead to angiogenesis induction. Tumor suppressor miRNAs, on the other hand, enhance anti-angiogenic response by targeting pro-angiogenic factors. Understanding the interaction between these miRNAs and their target mRNAs will help to unravel molecular mechanisms involved in CRC progression. The aim of this article is to review the current literature on angioregulatory miRNAs in CRC.

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Extracellular matrix metalloproteinase inducer (EMMPRIN) is a potential biomarker of angiogenesis in proliferative diabetic retinopathy

Abstract: These results suggest that EMMPRIN/MMPs/VEGF pathway is involved in PDR angiogenesis.

Cited by 34 publications

References 38 publications

The protective effects of lycopus lucidus turcz in diabetic retinopathy and its possible mechanisms

The protective effects of lycopus lucidus turcz in diabetic retinopathy and its possible mechanisms

Annexin A2 Promotes Development of Retinal Neovascularization through PI3K/ AKT Pathway

Angioregulatory microRNAs in Colorectal Cancer

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