Extracellular Matrix in Early Stages of Direct Somatic Embryogenesis in Leaves of Drosera spathulata

Bobák, M.; Šamaj, Jozef; Hlinková, E.; Hlavacka, Andrej; Ovečka, Miroslav

doi:10.1023/b:biop.0000022245.64929.8b

Cited by 26 publications

(17 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Several authors have shown that a reticularlike network mediates intercellular contacts among cells, and that a dense reticular network filling large intercellular "caves" forms during somatic embryogenesis (Oveèka and Bobak, 1999;Popielarska et al, 2006). The formation of the extracellular matrix surface network can be associated with a stress response in explants imposed by specific in vitro conditions (Bobák et al, 2004). We suppose that addition of C nanoparticles to cultivation medium influences intercellular interaction which, in turn, modifies differentiation of tissues and organs in calli cultures.…”

Section: Discussionmentioning

confidence: 99%

Reaction of Flax (Linum Usitatissimum L.) Calli Culture to Supplement of Medium by Carbon Nanoparticles

Kokina¹,

Sļedevskis²,

Gerbreders³

et al. 2012

Proceedings of the Latvian Academy of Sciences. Section B. Natural, Exact, and Applied Sciences

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 99%

Reaction of Flax (Linum Usitatissimum L.) Calli Culture to Supplement of Medium by Carbon Nanoparticles

Kokina¹,

Sļedevskis²,

Gerbreders³

et al. 2012

Proceedings of the Latvian Academy of Sciences. Section B. Natural, Exact, and Applied Sciences

View full text Add to dashboard Cite

“…This structure, which is referred to as extracellular matrix (ECM) or extracellular matrix surface network (ECMSN), has been found during the early stages of somatic embryogenesis and organogenesis e.g., in coconut [8] and maize [9]. The exact role of this structure is still not known, but its regulatory and coordinating functions during the early stages of morphogenesis have been suggested [8,[10][11][12][13].…”

Section: Introductionmentioning

confidence: 99%

Extracellular matrix surface network is associated with non-morphogenic calli of Helianthus tuberosus cv. Albik produced from various explants

Pilarska

Popielarska-Konieczna

Ślesak

et al. 2014

Acta Soc Bot Pol

View full text Add to dashboard Cite

Helianthus tuberosus is economically important species. To improve characters of this energetic plant via genetic modification, production of callus tissue and plant regeneration are the first steps. A new, potentially energetic cultivar Albik was used in this study to test callus induction and regeneration. Callus was produced on leaves, petioles, apical meristems and stems from field-harvested plants but was totally non-morphogenic. Its induction started in the cortex and vascular bundles as confirmed by histological analysis. The surface of heterogeneous callus was partially covered with a membranous extracellular matrix surface network visible in scanning and transmission electron microscopies. The results clearly indicate that: (i) the morphogenic capacity of callus in topinambur is genotype dependent, (ii) cv. Albik of H. tuberosus proved recalcitrant in in vitro regeneration, and (iii) extracellular matrix surface network is not a morphogenic marker in this cultivar.

show abstract

“…1 Šamaj et al, 2,3 reported, that the ECMSN plays an important morphoregulatory role during somatic embryogenesis and organogenesis, implying an active role in plant morphogenesis. According to Bobák et al, 4 the chemical composition and structural arrangement of the ECMSN on the cell surface indicate that it may play a fundamental role in cell-to-cell recognition and interaction, cell division and differentiation, and also in generation and maintenance of some traits in plant cell populations. There is not a large body of data confirming the occurrence of ECM during in vitro organogenesis.…”

mentioning

confidence: 99%

Are extracellular matrix surface network components involved in signalling and protective function?

Popielarska-Konieczna

Kozieradzka-Kiszkurno²,

Swierczynska³

et al. 2008

Plant Signaling & Behavior

View full text Add to dashboard Cite

Endosperm is an interesting model for in vitro experiments, because of its unique origin, development and ploidy level. Here we used Actinidia deliciosa endosperm-derived callus to investigate morphology, histology and chemistry of extracellular matrix (ECM) structures in morphogenically stable tissue from long-term culture. SEM and TEM analysis showed that ECM is a heterogenous layer which consists of amorphous, dark-staining material, osmiophilic granules and reticulated fibres outside the outer callus cell wall. This structure may serve as a structural marker of morphogenic competence in endosperm-derived callus, because of its presence on the surface of callus forming morphogenic domains and its disappearance during organ growth. Based on immunolabelling, histochemistry, solvent and enzyme treatments, we suggest that pectins and lipids are components of the ECM layer. These results might indicate protective, water retention and/or cell communication functions for this ECM layer.In several plants cultured in vitro, SEM analysis revealed that induction of morphogenesis is linked to the appearance of a fibrillar network referred to as the extracellular matrix surface network (ECMSN). 1 Šamaj et al., 2,3 reported, that the ECMSN plays an important morphoregulatory role during somatic embryogenesis and organogenesis, implying an active role in plant morphogenesis. According to Bobák et al., 4 the chemical composition and structural arrangement of the ECMSN on the cell surface indicate that it may play a fundamental role in cell-to-cell recognition and interaction, cell division and differentiation, and also in generation and maintenance of some traits in plant cell populations. There is not a large body of data confirming the occurrence of ECM during in vitro organogenesis. 5,6 Most data concern the formation, structure and chemical composition and function of ECM in somatic and androgenic embryogenesis (reviewed in refs. 6-10). In previous histological and SEM analysis of morphogenic endosperm-derived callus of A. deliciosa, we described the presence of a membranous layer covering the callus surface, which we termed extracellular matrix (ECM). 11 The present experiments employed SEM, TEM and histochemical analysis to study the structure and composition of material coating the callus surface.SEM revealed the presence of heterogeneous material covering the callus surface. A smooth membranous layer coated some parts of the callus surface (Fig. 1A), while other regions were covered by fibrillar structures and granules of mucilage-like secretion forming a network at site of cell-cell adhesion. Similar structures were observed during in vitro induction of somatic embryogenesis, androgenesis and organogenesis in different species (reviewed in refs. 7-13).TEM showed a layer composed of amorphous material with fibrillar and spherical components covering morphogenic cell clusters. The cell wall of non-embryogenic and senescent cells was also covered with ECM, but it differed in appearance: the layer was granular, with amo...

show abstract

Extracellular Matrix in Early Stages of Direct Somatic Embryogenesis in Leaves of Drosera spathulata

Cited by 26 publications

References 24 publications

Reaction of Flax (Linum Usitatissimum L.) Calli Culture to Supplement of Medium by Carbon Nanoparticles

Reaction of Flax (Linum Usitatissimum L.) Calli Culture to Supplement of Medium by Carbon Nanoparticles

Extracellular matrix surface network is associated with non-morphogenic calli of Helianthus tuberosus cv. Albik produced from various explants

Are extracellular matrix surface network components involved in signalling and protective function?

Contact Info

Product

Resources

About