Extracellular Matrix From Decellularized Wharton’s Jelly Improves the Behavior of Cells From Degenerated Intervertebral Disc

Penolazzi, Letizia; Pozzobon, Michela; Bergamin, Letícia Scussel; D’Agostino, Stefania; Francescato, Riccardo; Bonaccorsi, Gloria; Bonis, Pasquale De; Cavallo, Michele Alessandro; Lambertini, Elisabetta; Piva, Roberta

doi:10.3389/fbioe.2020.00262

Cited by 33 publications

(31 citation statements)

References 54 publications

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“…The Wharton's jelly extracellular matrix is partly comprised of glycosaminoglycans and collagen, similar to cartilage [29][30][31]. This relationship makes Wharton's jelly cells an excellent source for cartilage tissue engineering [32][33][34][35]. Chondrocytes and human Wharton's jelly cells also express aggrecan, type II collagen, and hyaluronic acid [29].…”

Section: Discussionmentioning

confidence: 99%

Umbilical Cord-Derived Wharton’s Jelly for Regenerative Medicine Applications: A Systematic Review

et al. 2021

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Musculoskeletal ailments affect millions of people around the world and place a high burden on healthcare. Traditional treatment modalities are limited and do not address underlying pathologies. Mesenchymal stem cells (MSCs) have emerged as an exciting therapeutic alternative and Wharton’s jelly-derived mesenchymal stem cells (WJSCs) are some of these. This review reports the clinical and functional outcomes of the applications of WJSCs in orthopedic surgery. A systematic review was conducted utilizing the Preferred Reporting Items for Systematic Reviews and Meta-analyses (PRISMA) guidelines. The studies that used culture-expanded, mesenchymal stem or stromal cells, MSCs and/or connective tissues procured from Wharton’s jelly (WJ), from January 2010 to October 2021, were included. Conventional non-operative therapies and placebos were used as comparisons. Six studies that directly discussed WJSCs use in an animal model or the basic scientific testing using an injury model were identified. Five publications studied cartilage injury, three studied degenerative disc disease, one was related to osteoarthritis, and one was related to osteochondral defects. The results of these studies suggested the benefits of WJSCs in the management of these orthopedic pathologies. To adequately assess the safety and efficacy of WJSCs in orthopedic surgery, further randomized controlled clinical studies are necessary.

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Section: Discussionmentioning

confidence: 99%

Umbilical Cord-Derived Wharton’s Jelly for Regenerative Medicine Applications: A Systematic Review

et al. 2021

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“…88 Supplementation of culture media with insulin transferrin-selenium, proline, dexamethasone, and pyruvate to a multi-lamellated outer and inner AF engineered tissue was able to promote the accumulation of collagen type I in the outer region and aggrecan and collagen type II the in inner region, replicating the ECM distribution of the native tissue. 89 Considering the beneficial effects on ECM components in maintaining and/or directing cell phenotype, 90 and degradation rate. 91 Given the importance of mechanical loading in disc degeneration and regeneration, bioreactor systems have been utilized to provide more physiologically relevant conditions for the development of functional IVD substitutes.…”

Section: Optimization Of Cells and Cues For Ivd Tementioning

confidence: 99%

“…Considering the beneficial effects on ECM components in maintaining and/or directing cell phenotype, 90 future strategies could incorporate decellularized ECM either within the polymer solution or post‐electrospinning to improve biocompatibility, mechanical stability, and degradation rate 91 . Given the importance of mechanical loading in disc degeneration and regeneration, bioreactor systems have been utilized to provide more physiologically relevant conditions for the development of functional IVD substitutes 92 .…”

Section: Electrospinningmentioning

confidence: 99%

Electrospinning and 3D bioprinting for intervertebral disc tissue engineering

et al. 2020

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Intervertebral disc (IVD) degeneration is a major cause of low back pain and represents a massive socioeconomic burden. Current conservative and surgical treatments fail to restore native tissue architecture and functionality. Tissue engineering strategies, especially those based on 3D bioprinting and electrospinning, have emerged as possible alternatives by producing cell-seeded scaffolds that replicate the structure of the IVD extracellular matrix. In this review, we provide an overview of recent advancements and limitations of 3D bioprinting and electrospinning for the treatment of IVD degeneration, focusing on future areas of research that may contribute to their clinical translation.

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“…The WJ matrix, mainly composed of glycosaminoglycans (GAGs) and collagen fibers, acts to prevent compression of the vessels [ 24 , 25 , 26 , 27 , 28 ]. The hydrated and viscous state of WJ is attributed to the high percentage of high molecular weight hyaluronic acid content, making WJ matrix an attractive option for regenerative medicine and matrix-based therapy [ 20 , 29 ]. In this context, substantial empirical clinical reports emphasize the regenerative therapeutic potential of devitalized umbilical cord for skin repair defect associated with bone exposure in medically fragile patients (i.e., diabetes, ischemia and osteomyelitis).…”

Section: Introductionmentioning

confidence: 99%

Antibacterial and Immunomodulatory Properties of Acellular Wharton’s Jelly Matrix

et al. 2022

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Of all biologic matrices, decellularized tissues have emerged as a promising tool in the field of regenerative medicine. Few empirical clinical studies have shown that Wharton’s jelly (WJ) of the human umbilical cord promotes wound closure and reduces wound-related infections. In this scope, we herein investigated whether decellularized (DC)-WJ could be used as an engineered biomaterial. In comparison with devitalized (DV)-WJ, our results showed an inherent effect of DC-WJ on Gram positive (S. aureus and S. epidermidis) and Gram negative (E. coli and P. aeruginosa) growth and adhesion. Although DC-WJ activated the neutrophils and monocytes in a comparable magnitude to DV-WJ, macrophages modulated their phenotypes and polarization states from the resting M0 phenotype to the hybrid M1/M2 phenotype in the presence of DC-WJ. M1 phenotype was predominant in the presence of DV-WJ. Finally, the subcutaneous implantation of DC-WJ showed total resorption after three weeks of implantation without any sign of foreign body reaction. These significant data shed light on the potential regenerative application of DC-WJ in providing a suitable biomaterial for tissue regenerative medicine and an ideal strategy to prevent wound-associated infections.

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Extracellular Matrix From Decellularized Wharton’s Jelly Improves the Behavior of Cells From Degenerated Intervertebral Disc

Cited by 33 publications

References 54 publications

Umbilical Cord-Derived Wharton’s Jelly for Regenerative Medicine Applications: A Systematic Review

Umbilical Cord-Derived Wharton’s Jelly for Regenerative Medicine Applications: A Systematic Review

Electrospinning and 3D bioprinting for intervertebral disc tissue engineering

Antibacterial and Immunomodulatory Properties of Acellular Wharton’s Jelly Matrix

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