Extracellular matrix dermatopontin modulates prostate cell growth in vivo

Abstract

Dermatopontin is a tyrosine-rich acidic extracellular matrix protein of 22 kDa with possible functions in cell-matrix interactions and matrix assembly. We have previously isolated mRNAs expressed in hormone-refractory, but not in hormone-sensitive, mouse mammary cancer by comparing the mRNAs expressed in either tumor. A partial mRNA sequence isolated was later proven to be a part of mouse dermatopontin mRNA sequence. Transfectants of mouse dermatopontin cDNA into PC-3 human prostate cancer cells enhanced tumor growth when those were implanted subcutaneously in nude mice compared with the controls. Those transfectants showed a prominent stroma compared with the controls. Localization of the targets of a fusion protein of mouse dermatopontin and alkaline phosphatase was in the stroma of the PC-3 tumor tissues, but not in the tumor cells themselves. Additionally, we have established mouse dermatopontin transgenic mice under the control of the rat probasin promoter. The prostatic dorsal lobes of dermatopontin transgenic mouse showed prostate intraepithelial neoplasia at the age of 11 months, but the control littermates did not. Epithelium of other prostatic lobes was not markedly different from that of the controls. In conclusion, dermatopontin may be involved in the pathogenesis and growth of the prostate cancer.

Androgen receptor (AR) positive vs negative roles in prostate cancer cell deaths including apoptosis, anoikis, entosis, necrosis and autophagic cell death

“…The interactions between cells and the extra-cellular matrix (ECM) play very important roles in the cell growth and cell death 101–103 . Aside from attachment to ECM, epithelial cells and/or endothelial cells could be induced by another type of cell death 104 , named as anoikis 105 , that resulted from insufficient cell-matrix interactions.…”

Gene Expression of Mesothelioma in Vinylidene Chloride–exposed F344/N Rats Reveal Immune Dysfunction, Tissue Damage, and Inflammation Pathways

“…Synucleins are neural proteins primarily expressed in neural tissues and cancers (George 2002). In several human cancers, including colorectal, esophageal, and breast cancer (Amsterdam et al ), SNCG is associated with tumorigenicity, cell proliferation and invasion (Ahmad et al 2007), and DPT is an extracellular matrix protein that has a stimulatory affect on tumor growth in prostate cancer (Takeuchi et al 2006). Several embryonic genes ( Plac8 , Wnt4, Gata5, Plau ) were increased in mesotheliomas of VDC-exposed rats compared to Fred-PE mesothelial cells, and Plac8 was increased in VDC associated mesotheliomas compared to spontaneous tumors.…”

Identification of Genes Differentially Expressed in the Ganglia of GrowingHaliotis asinina

“…In mammals, dermatopontin is in involved in the modulation of cell proliferation (Okamoto et al 1999, Takeuchi et al 2006. Although the role of dermatopontin in the H. asinina ganglia is unknown, its overall upregulation in the FG cohort is consistent with a role in cell proliferation.…”