Extracellular matrix constituents affect superficial gastric epithelial cell adhesion

Abstract

The interfoveolar and upper gastric pit cells become necrotic and slough off after superficial luminal injury to the gastric mucosa. The subsequent rapid epithelial restitution of the wound is dependent on an intact basal lamina upon which viable mucous cells migrate. Several lines of evidence suggest that migrating mucous cells recognize specific moieties in the basal lamina which would then affect restitution and the ability of the gastric mucosa to be repaired. Therefore, this study examined the effect of three individual protein constituents of the extracellular matrix, laminin, fibronectin and type IV collagen as well as a synthetic basal lamina, Matrigel, on adherence of mucous cells isolated from guinea-pig stomach to these substrates in culture. After 3 h, approximately 40% of the cells adhered to Matrigel, 25% to both collagen IV and fibronectin, but only about 10% to laminin and 3% to uncoated plastic substrates. Disruption of protein synthesis by pre-incubation with cyclohexamide significantly reduced adherence to Matrigel and collagen IV but not laminin, fibronectin or plastic substrates. These results suggest that gastric mucous cells have multiple receptors for extracellular matrix proteins (ligands) which influence the adherence and probably the migration of these cells. Furthermore, some of these receptors are synthesized in response to moieties in the substrate itself.

Rat Gastric Gelatinase Induction During Endotoxemia

“…The restoration of the gastric mucosa after a superficial injury is dependent not only on cellular proliferation but also on cell migration. Gastric mucous cells migrate to areas of injury to replace gastric pit cells which have sloughed (43). The migration of these mucosal cells is dependent on the presence of an intact basal lamina (44).…”

Human Caco-2 Intestinal Epithelial Motility Is Associated with Tyrosine Kinase and Cytoskeletal Focal Adhesion Kinase Signals

“…In vivo, cells interact with various matrix proteins likely to initiate integrin-mediated signals. We [6,7,17,42] and others [43][44][45][46] have previously observed that different purified matrix proteins have different effects on the cell phenotype. Certainly, it must be acknowledged that observations in isolated cell lines in tissue culture plastic dishes are not necessarily predictive of in vivo biology.…”