Extracellular matrix-based sticky sealants for scar-free corneal tissue reconstruction

Kim, Hyeonji; Jang, Je-Hwan; Han, Wonil; Hwang, Hyun-Jeong; Jang, Jinah; Kim, Joon‐Young; Cho, Dong‐Woo

doi:10.1016/j.biomaterials.2022.121941

Cited by 14 publications

(11 citation statements)

References 47 publications

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“…2C). 15,32 The preservation of collagen and GAG contents of cornea after decellularization process was proved by evaluating of HP content as a collagen indicator and GAG content which were not significantly different from contents in native cornea (Fig. 2D and E).…”

Section: Resultsmentioning

confidence: 88%

“…This irregular and disconnected basement membrane can prolong stromal haziness. 15 In addition, the epithelial layer in the Gel-PEG group was filled with epithelial cells with vacuolated cytoplasm (black arrows in Fig. 8A), whereas the untreated defects were congested with blood vessels (red arrow in Fig.…”

Section: Biomaterials Science Papermentioning

confidence: 94%

“…These observations are in line with our earlier in vitro findings, in which the bioactive components of corneal ECM promoted corneal stromal ECM genes (KERA and ALDH) in CSSCs, which are associated with a decline in myofibroblastic (Col1 A1, Col3 A1 and α-SMA) and vascular pericytes (α-SMA) markers. Upregulation of small molecules, including KERA in the stromal cornea ECM, can determine the size and uniformity of the collagen microarchitecture 15 and down-regulation of myofibroblast and angiogenesis markers are contributed to decrease in myofibroblast differentiation in CSSCs; thereby promote corneal transparency. 15…”

Section: Biomaterials Science Papermentioning

confidence: 99%

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ECM-based bioadhesive hydrogel for sutureless repair of deep anterior corneal defects

Borouman,

Sigaroodi,

Ahmadi Tafti

et al. 2024

Biomater. Sci.

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Section: Resultsmentioning

confidence: 88%

Section: Biomaterials Science Papermentioning

confidence: 94%

Section: Biomaterials Science Papermentioning

confidence: 99%

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ECM-based bioadhesive hydrogel for sutureless repair of deep anterior corneal defects

Borouman,

Sigaroodi,

Ahmadi Tafti

et al. 2024

Biomater. Sci.

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“…However, in failed healing with corneal scarring and vision impairment, accumu- lation of myofibroblasts and excessive secretion of ECM, including FN1, is seen in the damaged cornea. 25,26 Our present study found that HCPT could reduce cell viability of corneal fibroblasts in a dose-dependent manner. Additionally, 1 μg/mL HCPT suppressed fibroblast proliferation, and decreased the expression of TGF-β1-induced fibrotic gene expression (FN1, ACTA2) (Figure 2 and Figure 6A).…”

Section: Discussionmentioning

confidence: 99%

Hydroxycamptothecin and Substratum Stiffness Synergistically Regulate Fibrosis of Human Corneal Fibroblasts

Chen

Long

et al. 2023

ACS Biomater. Sci. Eng.

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Corneal fibrosis is a common outcome of inappropriate repair associated with trauma or ocular infection. Altered biomechanical properties with increased corneal stiffness is a feature of fibrosis that cause corneal opacities, resulting in severe visual impairment and even blindness. The present study aims to determine the effect of hydroxycamptothecin (HCPT) and matrix stiffness on transforming growth factor-β1 (TGF-β1)-induced fibrotic processes in human corneal fibroblasts (HTK cells). HTK cells were cultured on substrates with different stiffnesses (“soft”, ∼261 kPa; “stiff”, ∼2.5 × 103 kPa) and on tissue culture plastic (TCP, ∼106 kPa) and simultaneously treated with or without 1 μg/mL HCPT and 10 ng/mL TGF-β1. We found that HCPT induced decreased cell viability and antiproliferative effects on HTK cells. TGF-β1-induced expression of fibrosis-related genes (FN1, ACTA2) was reduced if the cells were simultaneously treated with HCPT. Substrate stiffness did not affect the expression of fibrosis-related genes. The TGF-β1 induced expression of FN1 on both soft and stiff substrates was reduced if cells were simultaneously treated with HCPT. However, this trend was not seen for ACTA2, i.e., the TGF-β1 induced expression of ACTA2 was not reduced by simultaneous treatment of HCPT in either soft or stiff substrate. Instead, HCPT treatment in the presence of TGF-β1 resulted in increased gene expression of keratocyte phenotype makers (LUM, KERA, AQP1, CHTS6) on both substrate stiffnesses. In addition, the protein expression of keratocyte phenotype makers LUM and ALDH3 was increased in HTK cells simultaneously treated with TGF-β1 and HCPT on stiff substrate as compared to control, i.e., without HCPT. In conclusion, we found that HCPT can reduce TGF-β1-induced fibrosis and promote the keratocyte phenotype in a substrate stiffness dependent manner. Thus, HCPT stimulation might be an approach to stimulate keratocytes in the appropriate healing stage to avoid or reverse fibrosis and achieve more optimal corneal wound healing.

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“…Moreover, decellularized membrane sheets combined with cytokines or new materials to achieve better tissue regeneration effects have been used in many tissue regeneration processes, such as dental tissue regeneration [ 7 ], bone regeneration [ 8 ], vascular reconstruction [ 9 ], nerve regeneration [ 10 ], and corneal regeneration [ 11 ]. Specifically, the common C-dECM sources for periodontal regeneration are the periodontal ligament stem cell sheet (PDLSC sheet) [ 12 ], the bone marrow mesenchymal stem cell sheet (BMSC sheet) [ 6 ], the human urogenic mesenchymal stem cell sheet (hUMSC sheet), and others such as dental folicle stem cells and L929 sheet-derived dECM can also be considered.…”

Section: Decm Derived From Different Sourcesmentioning

confidence: 99%

Advances Focusing on the Application of Decellularized Extracellular Matrix in Periodontal Regeneration

2023

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The decellularized extracellular matrix (dECM) is capable of promoting stem cell proliferation, migration, adhesion, and differentiation. It is a promising biomaterial for application and clinical translation in the field of periodontal tissue engineering as it most effectively preserves the complex array of ECM components as they are in native tissue, providing ideal cues for regeneration and repair of damaged periodontal tissue. dECMs of different origins have different advantages and characteristics in promoting the regeneration of periodontal tissue. dECM can be used directly or dissolved in liquid for better flowability. Multiple ways were developed to improve the mechanical strength of dECM, such as functionalized scaffolds with cells that harvest scaffold-supported dECM through decellularization or crosslinked soluble dECM that can form injectable hydrogels for periodontal tissue repair. dECM has found recent success in many periodontal regeneration and repair therapies. This review focuses on the repairing effect of dECM in periodontal tissue engineering, with variations in cell/tissue sources, and specifically discusses the future trend of periodontal regeneration and the future role of soluble dECM in entire periodontal tissue regeneration.

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Extracellular matrix-based sticky sealants for scar-free corneal tissue reconstruction

Cited by 14 publications

References 47 publications

ECM-based bioadhesive hydrogel for sutureless repair of deep anterior corneal defects

ECM-based bioadhesive hydrogel for sutureless repair of deep anterior corneal defects

Hydroxycamptothecin and Substratum Stiffness Synergistically Regulate Fibrosis of Human Corneal Fibroblasts

Advances Focusing on the Application of Decellularized Extracellular Matrix in Periodontal Regeneration

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