Extracellular Matrix and Dermal Fibroblast Function in the Healing Wound

Tracy, Lauren E.; Minasian, Raquel A.; Caterson, Edward J.

doi:10.1089/wound.2014.0561

Cited by 677 publications

(619 citation statements)

References 188 publications

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“…Previous studies suggested a role for DPT in skin wound healing and ECM maturation (70,71). Strikingly, DPT deficiency protected the liver from increased collagen deposition in the CCl 4 -induced fibrosis model (Figure 4), similar as in cutaneous fibrosis (30,58).…”

Section: Discussionsupporting

confidence: 63%

Interspecies NASH disease activity whole-genome profiling identifies a fibrogenic role of PPARα-regulated dermatopontin

et al. 2017

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Section: Discussionsupporting

confidence: 63%

Interspecies NASH disease activity whole-genome profiling identifies a fibrogenic role of PPARα-regulated dermatopontin

et al. 2017

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“…Furthermore, at this stage, the fibroblasts also undergo apoptosis resulting in the granulation tissue being replaced by collagen. 17 In cases of a hyperglycemic or diabetic condition, an increase in inflammatory mediators and advanced glycation end products (AGEs) occurs. AGEs are formed during the pathogenic process of diabetes, binding to the receptor of AGEs (RAGE).…”

Section: Discussionmentioning

confidence: 99%

“…An increased number of fibroblasts can stimulate the synthesis of collagen which plays an important role in tissue regeneration. 17 This study aimed to analyze the increase in the amount of collagen in traumatic ulcer after the application of liquid smoke coconut shell in subjects suffering from diabetes.…”

Section: 2mentioning

confidence: 99%

Increase of collagen in diabetes-related traumatic ulcers after the application of liquid smoke coconut shell

Surboyo

Arundina

Rahayu

2017

Dent. J. (Maj. Ked. Gigi)

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“…19,20 Manipulation and augmentation of the ECM components in chronic wound healing therapies currently use biochemical products such as acellular dermal matrices, wound dressings, tissue scaffolds, and topical products that contain ECM proteins such as collagen, hyaluronan, or elastin. 21 Hyperpolarization of the cell membrane in these experiments could be emitting a force that is affecting the poorly understood endogenous bioelectrical cues of the cell that ultimately lead to igniting mechanosensors in the cells causing changes in the cytoskeleton, which guide the directional migration seen in these fibroblasts. 22,23 Could the addition of this bioelectric field (as a footbath/bath) to the current biochemical products used in chronic wound therapies offer more success in treating chronic wounds?…”

Section: Discussionmentioning

confidence: 99%

Bioelectric Field Enhancement: The Influence on Membrane Potential and Cell Migration In Vitro

Purnell

Skrinjar

2016

Advances in Wound Care

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Objective:The extracellular matrix consists of critical components that affect fibroblast polarization and migration. The existence of both intrinsic and extrinsic electrical signals that play essential roles in the development, physiology, regeneration, and pathology of cells was discovered over a century ago. In this study, we study how the Bioelectric Field Enhancement (BEFE) device and its generated electromagnetic field (EMF) by continuous direct current (DC) significantly affect the membrane potential and cell migration of fibroblasts in vitro. Approach: This is an experimental analysis of membrane potential and cell migration of murine fibroblasts when grown in treated media that has been reconstituted with an aqueous solution that has been exposed to an EMF, which is generated by this device versus fibroblasts grown in identically prepared control media that has not been exposed to the EMF. Results: The growth of fibroblasts in the treated media shows a strong percent change in polarization of the plasma membrane and significant increase in cell migration compared to control groups. Innovation: These experiments show the potential for an adjunct wound care therapy using a continuous DC EMF application through a medium of water. Conclusion: Growth media that was reconstituted with an aqueous solution that had been exposed to this DC derived EMF shows significant changes in cell polarity and cell migration of fibroblasts in vitro. The BEFE device has shown enhanced chronic wound healing in anecdotal reports from patients globally for decades when used as a footbath/bath and could lead to a novel EMF application in wound healing.Keywords: bioelectrodynamics, membrane potential, cell migration, chronic wounds INTRODUCTIONElectromagnetic fields (EMFs) activate multiple signaling pathways that are instrumental in cell polarization, migration, and wound healing. [1][2][3][4] EMFs work in conjunction with and also override other signaling cues of cell migration such as: chemoattractant gradients, injury stimulation, contact inhibition, wound void, mechanical forces, and population pressure. 1,[5][6][7] Injured and inflamed cells are contained in a wound, and transmembrane potential differences have been found to exist in both injured and proliferating cells. 8 differentiation, and these magnetic fields also affect the changing gradients of Vmem that are produced and sensed by both excitable and nonexcitable cells in living organisms. 6,8,10,11 Inadequate and unorganized cell migration is characteristic of chronic wounds and to heal, cells must migrate directionally and in an organized way for granulation tissues to begin to develop. 2 EMF therapies in both in vitro and in vivo wound care research have been explored for decades and are currently being conducted with vibrating probe techniques that apply a flow of positive charge directed toward the wound using direct current (DC) or monophasic/biphasic pulsed electrical currents. 5,12 Low frequency alternating current (AC) has not been used with much success sin...

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Extracellular Matrix and Dermal Fibroblast Function in the Healing Wound

Cited by 677 publications

References 188 publications

Interspecies NASH disease activity whole-genome profiling identifies a fibrogenic role of PPARα-regulated dermatopontin

Interspecies NASH disease activity whole-genome profiling identifies a fibrogenic role of PPARα-regulated dermatopontin

Increase of collagen in diabetes-related traumatic ulcers after the application of liquid smoke coconut shell

Bioelectric Field Enhancement: The Influence on Membrane Potential and Cell Migration In Vitro

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