Extracellular laccase production during hyphal interactions between Trichoderma sp. and Shiitake, Lentinula edodes

Savoie, Jean‐Michel; Mata, Gerardo; Billette, Christophe

doi:10.1007/s002530051218

Cited by 67 publications

(40 citation statements)

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“…Different [1] and if their mycelium has colonised enough space before contact [13]. It is commonly observed that a brownish pigment is produced in the contact zone between the two mycelia when L. edodes rejects the attack of Trichoderma spp.…”

mentioning

confidence: 99%

“…L. edodes reject the attack of Trichoderma spp. by an over-production of laccases induced by extracellular metabolites of Trichoderma [13] and the formation of a brown line at the contact between the mycelia. Laccases are also induced by water soluble lignin derivatives after a period of adaptation and favour the mycelial growth of L. edodes in the presence of these compounds [7] [15] and could partly act as both a slow releasing C source and a protecting component.…”

mentioning

confidence: 99%

“…This reaction is associated with an over-production of laccases induced by extracellular metabolites of Trichoderma spp. [13].…”

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confidence: 99%

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Reductions in the incidence of Trichoderma spp. using substrate supplementation with peat and an alternative spawn during cultivation of Lentinula edodes on pasteurised wheat straw

Mata¹,

Savoie²,

Delpech³

et al. 1998

Agronomie

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confidence: 99%

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confidence: 99%

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Reductions in the incidence of Trichoderma spp. using substrate supplementation with peat and an alternative spawn during cultivation of Lentinula edodes on pasteurised wheat straw

Mata¹,

Savoie²,

Delpech³

et al. 1998

Agronomie

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“…일반적으로 백색부후균은 셀룰로오스, 헤미셀룰로오스 및 리그닌을 모두 분해하기 때문에, 부후가 진행됨에 따라 세포벽 은 내강으로부터 얇아지는 박벽화 현상이 나타나고 결국 중간 층까지도 분해된다 (Wilcox 1968(Wilcox , 1973. 표고균도 백색부후균 으로 셀룰로오스를 분해하는 엔도글루카나제(endoglucanase), 엑소셀로바이오하이드롤아제(exocellobiohydrolase), 베타글루 코시다아제(β-glucosidase), 리그닌의 페놀을 산화시키는 락카 제(laccase) (Savoie et al, 1998) (Fig. 7) 배지 표면에서는 자실체가 발생하였지만, 검정색으 로 변한 표면에서는 자실체가 발생하지 않았다 (Fig.…”

Section: 결과 및 고찰unclassified

“…이 효소는 표고자실체의 주름살에서 멜라린 생성에도 관여한다 (Sato et al, 2009). 한편 이 갈변현상은 표고균사가 Trichoderma 같은 병원균이나 유전형질이 다른 표고 균주와 만 났을 때에도 일어난다 (Savoie et al, 1998) …”

Section: 결과 및 고찰unclassified

Morphological Characteristics of Decomposition and Browning of Oak Sawdust Medium for Ground Bed Cultivation of Lentinula edodes

구창덕¹,

이선정²,

이화용³

2013

The Korean Journal of Mycology

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: This study investigated the internal and external morphological characteristics of decomposition and browning of oak sawdust medium for ground bed cultivation of Lentinula edodes. Within fifty days after L. edodes inoculation, surface hyphae on the bed browned. In 110 days, the fungal hyphae occupied and decomposed wood fibers, vessels and parenchymatous cells from the inside as white profuse hyphal mass was amorphously dissolving the saw dust particles from the outer surface. Most of the white hyphal bed surface became cleanly brown, however, some colony surface became blackened and slimy with contaminating bacteria, hyphae and spores. The brown layer was ca. 0.34 mm thick with highly dense and white hyphal mass beneath, whereas the blackened layer was ca. 1.17 mm thick with shrunken hyphae and less decomposed sawdust particles beneath. The surface hardness of the brown surface was ca. 0.73 kgf/cm 2 , soft and resilient, while that of the blackened was ca. 0.91 kgf/cm 2 , hard and nonresilient. By 150 days Lentinula edodes mushrooms fruited only on the brown surface and not on the blackened medium.

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Towards Higher Laccase Activities Produced by Aquatic Ascomycetous Fungi Through Combination of Elicitors and an Alternative Substrate

Junghanns¹,

Parra‐Saldívar²,

Keshavarz³

et al. 2008

Engineering in Life Sciences

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Laccases are versatile biocatalysts with various potential biotechnological applications, e.g. the treatment of industrial waste waters, the detoxification of environmental pollutants, or the functionalization of renewable polymeric materials. Central composition experimental design and response surface methodology was applied to optimize the production of laccase by the aquatic ascomycetous fungi, Phoma sp. UHH 5‐1‐03 and Coniothyrium sp. Kl‐S5, in shake flasks. A complex plant‐based medium (tomato juice) and two elicitors (Remazol Brilliant Blue R [RBBR] and CuSO4) were tested in combination at three concentrations. The highest laccase activity of 6322 ± 403 U/L was achieved on day 9 for Phoma sp. Coniothyrium sp. exerted a maximum laccase activity of 3035 ± 111 U/L on day 4. Optimal conditions were 30 % tomato juice and 450 mg/L RBBR for both strains. A concentration of 250 μM CuSO4 led to highest laccase activities in cultures of Coniothyrium sp., and 50 μM CuSO4 was most effective for Phoma sp. A remarkable synergistic effect of tomato juice and RBBR on laccase production was observed for both strains. The upscaling potential of the optimal induction conditions was demonstrated in a lab‐scale fermenter which resulted in maximum activities of 11030 ± 177 U/L on day 6 for Phoma sp. and 11530 ± 161 U/L on day 9 for Coniothyrium sp. This study therefore presents a promising alternative for laccase production in ascomycetes based on a cheap complex substrate in combination with two elicitors.

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Extracellular laccase production during hyphal interactions between Trichoderma sp. and Shiitake, Lentinula edodes

Cited by 67 publications

References 12 publications

Reductions in the incidence of Trichoderma spp. using substrate supplementation with peat and an alternative spawn during cultivation of Lentinula edodes on pasteurised wheat straw

Reductions in the incidence of Trichoderma spp. using substrate supplementation with peat and an alternative spawn during cultivation of Lentinula edodes on pasteurised wheat straw

Morphological Characteristics of Decomposition and Browning of Oak Sawdust Medium for Ground Bed Cultivation of Lentinula edodes

Towards Higher Laccase Activities Produced by Aquatic Ascomycetous Fungi Through Combination of Elicitors and an Alternative Substrate

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