Extracellular fungal polyol lipids: A new class of potential high value lipids

Garay, Luis A.; Sitepu, Irnayuli R.; Čajka, Tomáš; Xu, Jian; Teh, Hui Ean; German, J. Bruce; Pan, Zhongli; Dungan, Stephanie R.; Block, David E.; Boundy‐Mills, Kyria

doi:10.1016/j.biotechadv.2018.01.003

Cited by 45 publications

(52 citation statements)

References 77 publications

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“…As shown in Fig. 4A, when the medium contained 120.0 g/L glucose, both the intercellular lipid content and the extracellular PEFA titer reached the highest amount (51.4% and 14.1 g/L, respectively) and cell dry weight was 31.0 g/L within 144 h. Notably, this extracellular PEFA titer (14.1 g/L) were higher than those of other reported Rhodotorula strains from glucose at flask level [12], such as R . aff.…”

Section: Resultsmentioning

confidence: 70%

“…Notably, 3-hydroxyhexadecanoic acid (3-OH-C 16:0 ) and 3-hydroxyoctadecanoic acid (3-OH-C 18:0 ) were also detected in the intracellular components. This suggested that the hydroxylation of fatty acids in the strain P4R5 reacted inside the cell, and this process was probably catalyzed an cytochrome P450 monooxygenase bound to the endoplasmic reticulum membrane according to the reported sophorolipid synthesis pathway and the genetic studies in the Rhodotorula strains [12, 27]. Based on the results above, the chain lengths of the 3-hydroxy fatty acids of the PEFA from the strain P4R5 were C14, C16 and C18.…”

Section: Resultsmentioning

confidence: 81%

“…According to the previous literatures and the structure analyzed above (Table 2 and Fig. 3) [12, 27], the PEFA biosynthetic pathway in R. paludigena P4R5 is probable that the formation of the fatty acid portion of PEFA undergoes a similar biosynthetic pathway to that of triacylglycerol, followed by hydroxylation and esterification with a polyol group of mannitol. For fatty acid synthesis, inulin and glucose share the same metabolic pathways, because the inulin hydrolysis products are mainly fructose and a small amount glucose [20].…”

Section: Resultsmentioning

confidence: 82%

“…2 confirmed that the yeast strain P4R5 belonged to the species R. paludigena . So far, the vast majority of the PEFA secreting yeast strains, mostly belonging to genus Rhodotorula , were isolated from plant surfaces [12]. Therefore, R. paludigena P4R5 isolated from deep sea was a new producer of extracellular PEFA and intracellular lipids.

Fig.…”

Section: Resultsmentioning

confidence: 99%

“…Among these, polyol esters of fatty acids (PEFA) are composed of an acetylated ( R )-3-hydroxy fatty acid esterified through the carboxyl end to a 5 or 6 carbon polyol, typically d -mannitol or d -arabitol, with varying degrees of acetylation [11]. Intriguingly, the PEFA secreting strains are mostly belong to the genus Rhodotorula [12]. Specifically, Rhodotorula babjevae [13], Rhodotorula taiwanensis [14], Rhodotorula aff.…”

Section: Introductionmentioning

confidence: 99%

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Efficient simultaneous production of extracellular polyol esters of fatty acids and intracellular lipids from inulin by a deep-sea yeast Rhodotorula paludigena P4R5

et al. 2019

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Background Polyol esters of fatty acids (PEFA) are a kind of promising biosurfactants and mainly secreted by Rhodotorula strains. In addition, some strains of Rhodotorula are reliable producers of microbial lipid. Therefore, it is feasible to establish a one step fermentation process for efficient simultaneous production of PEFA and microbial lipids by a suitable Rhodotorula strain. Results A newly isolated deep-sea yeast, Rhodotorula paludigena P4R5, was shown to simultaneously produce high level of intracellular lipid and extracellular PEFA. Under the optimized conditions, it could yield 48.5 g/L of PEFA and 16.9 g/L of intracellular lipid within 156 h from inulin during 10-L batch fermentation. The PEFA consisting of a mixture of mannitol esters of 3-hydroxy C 14 , C 16 and C 18 fatty acids with variable acetylation showed outstanding surface activity and emulsifying activity, while the fatty acids of the intracellular lipid were mainly C 16 and C 18 and could be high-quality feedstock for biodiesel production. Conclusion The deep-sea yeast strain R. paludigena P4R5 was an excellent candidate for efficient simultaneous of biosurfactants and biodiesel from inulin. Our results also suggested that the establishment of fermentation systems with multiple metabolites production was an effective approach to improve the profitability.

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Section: Resultsmentioning

confidence: 70%

Section: Resultsmentioning

confidence: 81%

Section: Resultsmentioning

confidence: 82%

Fig.…”

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Efficient simultaneous production of extracellular polyol esters of fatty acids and intracellular lipids from inulin by a deep-sea yeast Rhodotorula paludigena P4R5

et al. 2019

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Liamocin biosynthesis is induced by an autogenous host acid activation in Aureobasidium melanogenum

Zhang,

Wei,

Wang

et al. 2023

Biotechnology Journal

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It has been known that maximal liamocin production must be carried out at low environmental pH (around 3.0). In this study, it was found that the low pH was mainly caused by the secreted citric acid which is one precursor of acetyl‐CoA for liamocin biosynthesis. Determination of citric acid in the culture, deletion, complementation and overexpression of the CEXA gene encoding specific citrate exporter demonstrated that the low pH was indeed caused by the secreted citric acid. Deletion, complementation and overexpression of the ACL gene encoding ATP‐citric acid lyase and effects of different initial pHs and added citric acid showed that the low pH in the presence of citric acid were suitable for lysis of intracellular citric acid, liamocin production and expression of the PACC gene encoding the pH signaling transcription factor PacC. This meant that the PACC gene was an acid‐expression gene. Deletion, complementation and overexpression of the PACC gene indicated that expression of the key gene cluster GAL1‐EST1‐PKS1 for liamocin biosynthesis was driven by the pH signaling transcription factor PacC and there was weak nitrogen catabolite repression on liamocin biosynthesis at the low pH. That was why liamocin biosynthesis was induced at the low pH in the presence of citric acid. The mechanisms of the enhanced liamocin biosynthesis by the autogenous host acid activation, together with the pH signaling pathway, were proposed.This article is protected by copyright. All rights reserved

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