Extracellular Expression in Aspergillus niger of an Antibody Fused to Leishmania sp. Antigens

Magaña-Ortíz, Denis; Fernández, Francisco; Loske, Achim M.; Gómez-Lim, Miguel Á.

doi:10.1007/s00284-017-1348-1

Cited by 16 publications

(14 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Filamentous fungi serve as a cell platform mainly for the production of large-scale industrial enzymes. However, due to certain features, such as very fast and strong growth and the ability to release large amounts of proteins directly to the substrate, this platform is used more widely for the production of recombinant proteins [17, 18]. It was reported that fungi such as Aspergillus niger were able to produce and secrete c.a.…”

Section: Comparison Among the Production Systems Of Recombinant Prmentioning

confidence: 99%

A Brief Reminder of Systems of Production and Chromatography-Based Recovery of Recombinant Protein Biopharmaceuticals

Owczarek

Gerszberg

Hnatuszko-Konka

2019

BioMed Research International

105

103

View full text Add to dashboard Cite

Recombinant proteins are produced for various applications in laboratory and industrial settings. Among them, therapeutic applications have evolved into a mature field in recent years, affecting the face of contemporary medical treatment. This, in turn, has stimulated an ever-greater need for innovative technologies for the description, expression, and purification of recombinant protein biopharmaceuticals. Therefore, many biopharmaceuticals are synthesized in heterologous systems to obtain satisfactory yields that cannot be provided by natural sources. As more than 35 years has passed since the first recombinant biopharmaceutical (human insulin) successfully completed clinical trials in humans, we provide a brief review of the available prokaryotic and eukaryotic expression systems, listing the advantages and disadvantages of their use. Some examples of therapeutic proteins expressed in heterologous hosts are also provided. Moreover, technologies for the universal extraction of protein molecules are mentioned here, as is the methodology of their purification.

show abstract

Section: Comparison Among the Production Systems Of Recombinant Prmentioning

confidence: 99%

A Brief Reminder of Systems of Production and Chromatography-Based Recovery of Recombinant Protein Biopharmaceuticals

Owczarek

Gerszberg

Hnatuszko-Konka

2019

BioMed Research International

105

103

View full text Add to dashboard Cite

show abstract

“…Recently, two fusion proteins each consisting of a highly antigenic protein from Leishmania sp. , an antibody specific for a receptor of dendritic cells, and a fragment of native glucoamylase A were generated in A. niger (Magana-Ortiz et al 2018). Both recombinant proteins may be used for immunization against Leishmaniasis and point to the potential of filamentous fungi as hosts for the generation of pharmaceutically relevant peptides.…”

Section: Production Of Heterologous Productsmentioning

confidence: 99%

Inducible promoters and functional genomic approaches for the genetic engineering of filamentous fungi

Kluge

Terfehr

Kück

2018

Appl Microbiol Biotechnol

View full text Add to dashboard Cite

In industry, filamentous fungi have a prominent position as producers of economically relevant primary or secondary metabolites. Particularly, the advent of genetic engineering of filamentous fungi has led to a growing number of molecular tools to adopt filamentous fungi for biotechnical applications. Here, we summarize recent developments in fungal biology, where fungal host systems were genetically manipulated for optimal industrial applications. Firstly, available inducible promoter systems depending on carbon sources are mentioned together with various adaptations of the Tet-Off and Tet-On systems for use in different industrial fungal host systems. Subsequently, we summarize representative examples, where diverse expression systems were used for the production of heterologous products, including proteins from mammalian systems. In addition, the progressing usage of genomics and functional genomics data for strain improvement strategies are addressed, for the identification of biosynthesis genes and their related metabolic pathways. Functional genomic data are further used to decipher genomic differences between wild-type and high-production strains, in order to optimize endogenous metabolic pathways that lead to the synthesis of pharmaceutically relevant end products. Lastly, we discuss how molecular data sets can be used to modify products for optimized applications.

show abstract

“…In addition, due to their saprophytic lifestyle, most filamentous fungi have already developed the ability to produce and secrete a vast amount of enzymes in order to break down and feed on organic matter [11]. Strains belonging to the genera Aspergillus, Trichoderma, and Neurospora are in fact widely used for production of recombinant proteins with industrial applications [12][13][14][15]. Several reviews have described the potentials of filamentous fungi in the production of pharmaceutical and other industrial proteins, as well as the genetic engineering approaches followed to maximize production levels [7,16,17].…”

Section: Introductionmentioning

confidence: 99%

Aspergillus: A Powerful Protein Production Platform

et al. 2020

View full text Add to dashboard Cite

Aspergilli have been widely used in the production of organic acids, enzymes, and secondary metabolites for almost a century. Today, several GRAS (generally recognized as safe) Aspergillus species hold a central role in the field of industrial biotechnology with multiple profitable applications. Since the 1990s, research has focused on the use of Aspergillus species in the development of cell factories for the production of recombinant proteins mainly due to their natively high secretion capacity. Advances in the Aspergillus-specific molecular toolkit and combination of several engineering strategies (e.g., protease-deficient strains and fusions to carrier proteins) resulted in strains able to generate high titers of recombinant fungal proteins. However, the production of non-fungal proteins appears to still be inefficient due to bottlenecks in fungal expression and secretion machinery. After a brief overview of the different heterologous expression systems currently available, this review focuses on the filamentous fungi belonging to the genus Aspergillus and their use in recombinant protein production. We describe key steps in protein synthesis and secretion that may limit production efficiency in Aspergillus systems and present genetic engineering approaches and bioprocessing strategies that have been adopted in order to improve recombinant protein titers and expand the potential of Aspergilli as competitive production platforms.

show abstract

Extracellular Expression in Aspergillus niger of an Antibody Fused to Leishmania sp. Antigens

Cited by 16 publications

References 26 publications

A Brief Reminder of Systems of Production and Chromatography-Based Recovery of Recombinant Protein Biopharmaceuticals

A Brief Reminder of Systems of Production and Chromatography-Based Recovery of Recombinant Protein Biopharmaceuticals

Inducible promoters and functional genomic approaches for the genetic engineering of filamentous fungi

Aspergillus: A Powerful Protein Production Platform

Contact Info

Product

Resources

About