Extracellular enzyme activities and nutrient availability during artificial groundwater recharge

Kolehmainen, Reija E.; Korpela, Jaana P.; Münster, U.; Puhakka, Jaakko A.; Tuovinen, Olli H.

doi:10.1016/j.watres.2008.10.048

Cited by 23 publications

(16 citation statements)

References 26 publications

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“…Our finding corroborated the results of previous studies on the effect of AGR with lake water on bacterial enzyme activities (Miettinen et al 1996). Kolehmainen et al (2009) suggested that the increase in extracellular biofilm hydrolytic activity during groundwater recharge with lake water was likely to result from increased fluxes of high molecular weight DOC which needs fractionation before microbial assimilation. However, the relationship between attached bacterial biomass and activity is not necessarily linear because the biofilm can reach a critical thickness that limits substrate diffusion at depth into the biofilm (Battin and Sengschmitt 1999).…”

Section: Effect Of Increasing Doc Fluxes On Sediment Biofilm In the Fsupporting

confidence: 91%

“…Increased DOC supply during stream and lake water infiltration was also found to stimulate respiratory and extracellular microbial activities implicated in organic matter processing (Miettinen et al 1996;Findlay et al 2003;Kolehmainen et al 2009) and modified the composition of bacterial communities (Kolehmainen et al 2007). However, most studies examining the effect of AGR focused on freeliving bacteria (Miettinen et al 1996;Kolehmainen et al 2009).…”

mentioning

confidence: 99%

“…However, most studies examining the effect of AGR focused on freeliving bacteria (Miettinen et al 1996;Kolehmainen et al 2009). In oligotrophic subsurface environments, most bacteria grow attached to sediments thereby forming a biofilm made of cell aggregates embedded in a matrix of extracellular polymeric substances (EPS) (Costerton et al 1995;Griebler et al 2002).…”

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confidence: 99%

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Response of sediment biofilm to increased dissolved organic carbon supply in groundwater artificially recharged with stormwater

et al. 2010

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Purpose Best management practices encompass diverse artificial groundwater recharge (AGR) systems that heavily rely upon the capacity of the soil and vadose zone to retain large quantities of organic matter generated during stormwater runoff on urban catchments. However, the supply of stormwater-derived dissolved organic carbon (DOC) at the water-table region of aquifers can enhance the rate of biogeochemical processes by fueling heterotrophic microbial metabolism. This study examined changes in the abundance and activity of sediment biofilm in response to increased DOC supply at the water table of an urban aquifer intentionally recharged with stormwater. Changes in microbial abundance and activity under field conditions were compared with those measured in laboratory slow filtration columns supplied with an easily biodegradable source of DOC. Materials and methods Biomass and activities of attached bacteria were measured on aquifer sediment samples collected at multiple depths below the groundwater table. The same microbial variables were measured on aquifer sediments introduced in slow filtration columns supplied with three distinct concentrations of biodegradable dissolved organic carbon (0.5, 1.5, and 2.5 mg l −1 of C-acetate). Results and discussion Biofilm was significantly more abundant and active beneath AGR sites than at control sites not intentionally recharged with stormwater. Individual cells also exhibited higher specific dehydrogenase and hydrolytic activities, and the protein content of biofilm was significantly increased by AGR with stormwater. The stimulation of the microbial community was all the more pronounced when the flux of DOC reaching the groundwater table was high. However, the increase in microbial biomass and activity at AGR sites was 7.5 times lower than that measured in slow filtration columns in which aquifer sediments were supplied with an easily biodegradable source of DOC. Conclusions Our results indicated that the soil and vadose zone at AGR sites efficiently retained the more labile fraction of DOC, thereby enabling the infiltration of large quantities of stormwater without excessively stimulating the growth of microbial biofilm in groundwater. However, our results showed that groundwater bacterial activity was stimulated by enhanced DOC fluxes. As microorganisms experience severe organic carbon limitation in most pristine aquifers, we suggest that groundwater biofilms could be used as sensitive indicators for assessing the DOC fluxes reaching aquifers.

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Section: Effect Of Increasing Doc Fluxes On Sediment Biofilm In the Fsupporting

confidence: 91%

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confidence: 99%

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confidence: 99%

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Response of sediment biofilm to increased dissolved organic carbon supply in groundwater artificially recharged with stormwater

et al. 2010

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“…In an oligotrophic, P-limited herbaceous wetlands of northern Belize, there was a strong correlation between macrophyte decomposition coefficient k-values and most of the enzymes (Rejmankova and Sirova 2007). To be brief, the extracellular enzymes functioned in a synergistic and cooperative way (Kolehmainen et al 2009), by which bioavailable phosphorus could be liberated. In our results, extracellular enzymes activities were also positively correlated with each other (Table 1).…”

Section: Discussionmentioning

confidence: 97%

Enhancement of sediment phosphorus release during a tunnel construction across an urban lake (Lake Donghu, China)

Wang

Xiao

et al. 2016

Environ Sci Pollut Res

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Tunnel construction in watershed area of urban lakes would accelerate eutrophication by inputting nutrients into them, while mechanisms underlying the internal phosphorus cycling as affected by construction events are scarcely studied. Focusing on two main pathways of phosphorus releasing from sediment (enzymatic mineralization and anaerobic desorption), spatial and temporal variations in phosphorus fractionation, and activities of extracellular enzymes (alkaline phosphatase, β-1,4-glucosidase, leucine aminopeptidase, dehydrogenase, lipase) in sediment were examined, together with relevant parameters in interstitial and surface waters in a Chinese urban lake (Lake Donghu) where a subaqueous tunnel was constructed across it from October 2013 to July 2014. Higher alkaline phosphatase activity (APA) indicated phosphorus deficiency for phytoplankton, as illustrated by a significantly negative relationship between APA and concentration of dissolved total phosphorus (DTP). Noticeably, in the construction area, APAs in both sediment and surface water were significantly lower than those in other relevant basins, suggesting a phosphorus supply from some sources in this area. In parallel, its sediment gave the significantly lower iron-bound phosphorus (Fe(OOH)∼P) content, coupled with significantly higher ratio of iron (II) to total iron content (Fe 2+ / TFe) and dehydrogenase activities (DHA). Contrastingly, difference in the activities of sediment hydrolases was not significant between the construction area and other basins studied. Thus, in the construction area, subsidy of bioavailable phosphorus from sediment to surface water was attributable to the anaerobic desorption of Fe(OOH)∼P rather than enzymatic mineralization. Finally, there existed a significantly positive relationship between chlorophyll a concentration in surface water and Fe(OOH)∼P content in sediment. In short, construction activities within lakes may interrupt cycling patterns of phosphorus across sediment-water interface by enhancing release of redox-sensitive phosphate, and thereby facilitating phytoplankton growth in water column.

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“…Microbial enzymatic activity is very sensitive to fl uctuation in external factors (Bandick and Dick, 1999) and is an inexpensive and easy technique to employ; for this reason enzymatic assays have been widely used to study the effect of environmental change in soil (e.g. Nannipieri et al, 1990;Acosta Martinez et al, 2003;Trasar-Cepeda et al, 2008;Kolehmainen et al, 2009). The potential for enzyme synthesis is determined by the abundance and composition of the microbial community present in soil, as a consequence changes in microbial communities associated with environmental factors should be refl ected in enzyme activity (Kandeler et al, 1996).…”

Section: Conventional Microbiological Methodsmentioning

confidence: 99%

Lowland Floodplain Responses to Extreme Flood Events: Long-Term Studies and Short-Term Microbial Community Response to Water Environment Impacts

Lillie

Soler

Smith

2012

Conservation and Management of Archaeological Sites

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Extracellular enzyme activities and nutrient availability during artificial groundwater recharge

Cited by 23 publications

References 26 publications

Response of sediment biofilm to increased dissolved organic carbon supply in groundwater artificially recharged with stormwater

Response of sediment biofilm to increased dissolved organic carbon supply in groundwater artificially recharged with stormwater

Enhancement of sediment phosphorus release during a tunnel construction across an urban lake (Lake Donghu, China)

Lowland Floodplain Responses to Extreme Flood Events: Long-Term Studies and Short-Term Microbial Community Response to Water Environment Impacts

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