Response of sediment biofilm to increased dissolved organic carbon supply in groundwater artificially recharged with stormwater

Abstract

Purpose Best management practices encompass diverse artificial groundwater recharge (AGR) systems that heavily rely upon the capacity of the soil and vadose zone to retain large quantities of organic matter generated during stormwater runoff on urban catchments. However, the supply of stormwater-derived dissolved organic carbon (DOC) at the water-table region of aquifers can enhance the rate of biogeochemical processes by fueling heterotrophic microbial metabolism. This study examined changes in the abundance and activity of sediment biofilm in response to increased DOC supply at the water table of an urban aquifer intentionally recharged with stormwater. Changes in microbial abundance and activity under field conditions were compared with those measured in laboratory slow filtration columns supplied with an easily biodegradable source of DOC. Materials and methods Biomass and activities of attached bacteria were measured on aquifer sediment samples collected at multiple depths below the groundwater table. The same microbial variables were measured on aquifer sediments introduced in slow filtration columns supplied with three distinct concentrations of biodegradable dissolved organic carbon (0.5, 1.5, and 2.5 mg l −1 of C-acetate). Results and discussion Biofilm was significantly more abundant and active beneath AGR sites than at control sites not intentionally recharged with stormwater. Individual cells also exhibited higher specific dehydrogenase and hydrolytic activities, and the protein content of biofilm was significantly increased by AGR with stormwater. The stimulation of the microbial community was all the more pronounced when the flux of DOC reaching the groundwater table was high. However, the increase in microbial biomass and activity at AGR sites was 7.5 times lower than that measured in slow filtration columns in which aquifer sediments were supplied with an easily biodegradable source of DOC. Conclusions Our results indicated that the soil and vadose zone at AGR sites efficiently retained the more labile fraction of DOC, thereby enabling the infiltration of large quantities of stormwater without excessively stimulating the growth of microbial biofilm in groundwater. However, our results showed that groundwater bacterial activity was stimulated by enhanced DOC fluxes. As microorganisms experience severe organic carbon limitation in most pristine aquifers, we suggest that groundwater biofilms could be used as sensitive indicators for assessing the DOC fluxes reaching aquifers.

Dissolved Organic Carbon Influences Microbial Community Composition and Diversity in Managed Aquifer Recharge Systems

“…The more oligotrophic vadose zone, which can range from a few meters to several hundreds of meters, represents a different regime where recalcitrant organic compounds become more prominent (27,49). Parallels can be drawn to analogous studies that characterize microbial abundance, community structure, metabolic status, and functional microbial groups and their response to toxic chemicals in the shallow infiltration zone of sediments (1,9,20,26,58). However, prior to the current study, little was known regarding seasonal or spatial variations in microbial community structure or physiochemical parameters shaping the microbial community specifically in MAR systems.…”

A modelling approach to quantify the influence of fine sediment deposition on biogeochemical processes occurring in the hyporheic zone

“…The selected value (3 r 10 x5 h x1 , Table 2) was in accordance with previous works (Van Cappellen and Wang, 2006;Wijsman et al, 2002;Canavan et al, 2006;Delmotte, 2007), supporting the validity of our parameterization. For the degradation of DOC, we used a k DOC of 3 h x1 which was higher than data commonly encountered in natural systems (Peyrard et al, 2011), because the source of DOC used in our system (acetate) was very easily consumed and metabolized by microorganisms in comparison with natural and more refractory DOC (Servais et al, 1989;Foulquier et al, 2011).…”

Microbial Purification in Well-Water Using UV-Vis LEDs and Monitoring Using Laser-Induced Fluorescence

“…Heterotrophic bacteria are not indicators of pathogenic conditions, but can cause infections in skin, lung and gastroenteritis (Bartram et al, 2003;Chopra & Houston, 1999, Helmer et al, 1997. Heterotrophic bacteria are present in all water types (Foulquier et al, 2011;Griebler & Lueders, 2009) and drinking water treatment does not remove or inactivate all heterotrophic organisms. Some are resistant because they may be in a spore or vegetative form with an impervious membrane, or may be protected from treatment as part of an aggregate (Geldreich, 1996).…”