Extracellular carbonic anhydrases of the stromatolite-forming cyanobacterium Microcoleus chthonoplastes

Kupriyanova, Elena V.; Villarejo, Arsenio; Markelova, A. G.; Герасименко, Л. М.; Zavarzin, G. A.; Samuelsson, Göran; Los, Dmitry A.; Пронина, Н. А.

doi:10.1099/mic.0.2006/003905-0

Cited by 64 publications

(27 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Similar results have been reported for bovine erythrocyte plasma membrane [20]. In contrast, the extra cellular CA of stromatolite forming cyanobacterium, Microcoleus cathonoplastes showed two peaks of activity at around pH 10.0 and 7.5 [1]. The optimum temperature for CA obtained from human erythrocyte plasma membrane was 35 o C and in other mammalian CA it was found to be .…”

Section: Resultssupporting

confidence: 84%

See 1 more Smart Citation

Characterization of carbonic anhydrase from diversified genus for biomimetic carbon-dioxide sequestration

et al. 2008

View full text Add to dashboard Cite

Diversifi ed group of bacteria were screened for carbonic anhydrase (CA) activity. Signifi cant CA activity was found in crude enzyme extracts of Enterobacter and Aeromonas isolates while minimal or negligible CA activity was observed in case of Shigella and Klebsiella spp. Optimization and characterization study of potent CA producing isolates revealed that the maximum enzyme activity of 3.86 EU/ml was observed in E. taylorae and the optimum pH range for enzyme stability was found to be 7.5-9.0 along with an optimum temperature range of 35-50 o C. The molecular mass of CA was 29-kDa indicating α-type with periplasmic and cytosolic location. Present investigation for the fi rst time reports CA in diversifi ed genus and optimized parameters for enhanced production of CA in Enterobacter sp. & Aeromonas sp. from fresh water bodies that inturn lay down grounds for exploitation of CA from E. taylorae as an effi cient catalyst for CO 2 sequestration within a bioreactor.

show abstract

Section: Resultssupporting

confidence: 84%

“…Carbon-dioxide (CO 2 ) is known to be responsible for an estimated 60% of the global warming from the green house gases generated by human activities [1]. Phenomenal rise in CO 2 concentration have led to disastrous consequences viz.…”

Section: Introductionmentioning

confidence: 99%

Characterization of carbonic anhydrase from diversified genus for biomimetic carbon-dioxide sequestration

et al. 2008

View full text Add to dashboard Cite

show abstract

“…Filamentous forms of Cyanobacteria are the primary structuring agents in fossil and modern-day stromatolites and microbialites (Allwood et al 2006;Kupriyanova et al 2007) found in shallow, often tropical, marine environments (Reid et al 2000;Sprachta et al 2001). They are also the main structuring elements in microbial mats common in extreme habitats, such as geothermal springs, hypersaline basins, hyperoligotrophic ponds, hot and cold deserts, and polar ice shelves (Hoffmann 1999;Ward and Castenholz 2000;Gorbushina 2007).…”

mentioning

confidence: 99%

Metagenomic profiling of Arctic microbial mat communities as nutrient scavenging and recycling systems

Varin

Lovejoy²,

Jungblut

et al. 2010

Limnology & Oceanography

View full text Add to dashboard Cite

By way of metagenomics and high-throughput pyrosequencing, we addressed the hypothesis that cyanobacterial mats in polar aquatic ecosystems maintain a nutrient-rich microenvironment via decomposition and scavenging processes. Analysis of more than 592,554 genomic deoxyribonucleic acid (DNA) reads (total of 11.5 million base pairs) showed that the ribosomal and protein-coding genes of two High Arctic ice-shelf mat communities were dominated by Proteobacteria, not Cyanobacteria, which implies a broad range of bacterial decomposition and nutrient recycling processes in addition to phototrophy. Principal component analysis of genes for light-, nitrogen-, and phosphorus-related processes provided evidence of partitioning of mat function among taxonomically different constituents of the mat consortia. Viruses were also present (notably Alpha-, Beta-, Gammaproteobacteria phages and cyanophages), which likely contribute to cellular lysis and recycling, as well as other Bacteria, Archaea, and microbial eukaryotes. Nitrogen-related genes were dominated by ammoniumassimilation systems, implying that the microbial mats are sites of intense mineralization, but not N-oxidation, since nitrification genes were absent. Nutrient scavenging systems were detected, including genes for transport proteins and enzymes for converting larger molecules into more readily assimilated inorganic forms (allantoin degradation, cyanate hydrolysis, exophosphatases, phosphonatases). Metagenomic profiling results underscore the rich diversity of microbial life even in extreme polar habitats, and the capability of mat consortia to retain and recycle nutrients in the benthic microenvironment.

show abstract

“…However, the cyanobacteria promote this activity only pericellularly. The internal pH of these organisms is neutral -their CA promotes external alkalinisation by OH − excretion, whereas fixing CO 2 from HCO − 3 generates pericellular CaCO 3 precipitation (Kupriyanova et al 2007). In other words, cyanobacteria promote the intense capture of inorganic carbon in the form of CO 2 or HCO − 3 not only by photosynthetic activity during blooms (similar to other microorganisms), but also by the extra cellular activity of CA, which alkalinises their environment.…”

Section: Alkaline Lakes and The Biogeochemical Processes Involvedmentioning

confidence: 99%

“…For this reason, these organisms are particularly efficient in raising the pH of the water. According to Kupriyanova et al (2007), the intensity of cyanobacterial CA activity is strictly controlled by pH, with a maximum at pH 9.8, which is the ideal pH for these organisms. Thus, at pH > 9.8 the CA probably becomes increasingly less effective at raising alkalinity.…”

Section: Alkaline Lakes and The Biogeochemical Processes Involvedmentioning

confidence: 99%

Biogeochemical processes and the diversity of Nhecolândia lakes, Brazil

Almeida

Calijuri

Falco

et al. 2011

An. Acad. Bras. Ciênc.

Self Cite

View full text Add to dashboard Cite

The Pantanal of Nhecolândia, the world's largest and most diversified field of tropical lakes, comprises approximately 10,000 lakes, which cover an area of 24,000 km 2 and vary greatly in salinity, pH, alkalinity, colour, physiography and biological activity. The hyposaline lakes have variable pHs, low alkalinity, macrophytes and low phytoplankton densities. The saline lakes have pHs above 9 or 10, high alkalinity, a high density of phytoplankton and sand beaches. The cause of the diversity of these lakes has been an open question, which we have addressed in our research. Here we propose a hybrid process, both geochemical and biological, as the main cause, including (1) a climate with an important water deficit and poverty in Ca 2+ in both superficial and phreatic waters; and (2) an elevation of pH during cyanobacteria blooms. These two aspects destabilise the general tendency of Earth's surface waters towards a neutral pH. This imbalance results in an increase in the pH and dissolution of previously precipitated amorphous silica and quartzose sand. During extreme droughts, amorphous silica precipitates in the inter-granular spaces of the lake bottom sediment, increasing the isolation of the lake from the phreatic level. This paper discusses this biogeochemical problem in the light of physicochemical, chemical, altimetric and phytoplankton data.

show abstract

Extracellular carbonic anhydrases of the stromatolite-forming cyanobacterium Microcoleus chthonoplastes

Cited by 64 publications

References 29 publications

Characterization of carbonic anhydrase from diversified genus for biomimetic carbon-dioxide sequestration

Characterization of carbonic anhydrase from diversified genus for biomimetic carbon-dioxide sequestration

Metagenomic profiling of Arctic microbial mat communities as nutrient scavenging and recycling systems

Biogeochemical processes and the diversity of Nhecolândia lakes, Brazil

Contact Info

Product

Resources

About