Extracellular calcium and CaSR drive osteoinduction in mesenchymal stromal cells

The proangiogenic potential of a novel calcium releasing biomaterial: Impact on cell recruitment

“…As we have recently shown that extracellular calcium signaling mediated by CaSR can also modulate the osteoinduction of mesenchymal stem cells in vitro [45], this approach, based on the release of calcium ions may serve to support both angiogenesis and osteogenesis in vivo. As to explore this dual potential role we are currently testing these matrices in an orthotopic bone defect model in vivo.…”

Microdamage induced calcium efflux from bone matrix activates intracellular calcium signaling in osteoblasts via L-type and T-type voltage-gated calcium channels

“…This result signifies strong involvement of VGCC on [Ca 2+ ] I modulation. Bepridil could not completely abolish baseline [Ca 2+ ] I response, and this suggests that other underlying mechanisms exist in MC3T3-E1, such as intracellular calcium stores or calcium sensing receptor (CaSR) which can also respond to [Ca 2+ ] E perturbations and release calcium ion into the cytosol [23,24]. However, the calcium oscillations were strongly dependent on VGCCs as they were close to completely abolished with Bepridil treatment.…”

Clinical and Molecular Perspectives of Reparative Dentin Formation

“…An activated CaSR elicits intracellular signaling pathways that ultimately lead to migration, proliferation and differentiation of cells 106 . Recently, it was shown that CaSR mediates osteogenic differentiation and mineralization of bone marrow mesenchymal stromal cells 107 . However, the presence of CaSR in bone-forming cells is controversial 108 , and osteoblasts derived from CaSR-null mice remained to possess osteogenic differentiation potential 109,110 .…”