Extracellular Ca2+ influx is crucial for the early embryonic development of the sea urchin Echinometra lucunter

Leite, Jocelmo Cássio de Araújo; Marques‐Santos, Luis Fernando

doi:10.1002/jez.b.21450

Cited by 2 publications

(2 citation statements)

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“…Domino and Garbers (1989), for example, reported that the acrosome reaction induced by the fucose‐sulfate glycoconjugate component of sea urchin egg jelly coat lead to phospholipid turnover in the entire spermatozoa membrane in a calcium‐dependent manner. Previously, data from our group showed that extracellular calcium is essential at the moment gamete contact and is required for successful fertilization in the sea urchin E. lucunter (de Araujo Leite and Marques‐Santos, 2012). We report here that pre‐incubation of spermatozoa in calcium‐free medium (Ca 2+ ‐free/ASW) did not decrease fertilizing capacity of spermatozoa.…”

Section: Discussionmentioning

confidence: 99%

Involvement of ABCB1 and ABCC1 transporters in sea urchin Echinometra lucunter fertilization

Silva-Neta¹,

Torrezan²,

Leite³

et al. 2012

Molecular Reproduction Devel

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Fertilization is an ordered sequence of cellular interactions that promotes gamete fusion to form a new individual. Since the pioneering work of Oskar Hertwig conducted on sea urchins, echinoderms have contributed to the understanding of cellular and molecular aspects of the fertilization processes. Studies on sea urchin spermatozoa reported the involvement of a plasma membrane protein that belongs to the ABC proteins superfamily in the acrosome reaction. ABC transporters are expressed in membranes of eukaryotic and prokaryotic cells, and are associated with the transport of several compounds or ions across biomembranes. We aimed to investigate ABCB1 and ABCC1 transporter activity in sea urchin spermatozoa and their involvement in fertilization. Our results indicate that Echinometra lucunter spermatozoa exhibit a low intracellular calcein accumulation (18.5% stained cells); however, the ABC blockers reversin205, verapamil, and MK571 increased dye accumulation (93.0-96.6% stained cells). We also demonstrated that pharmacologically blocking ABCB1 and ABCC1 decreased spermatozoa fertilizing capacity (70% inhibition), and this phenotype was independent of extracellular calcium. These data suggest that functional spermatozoa ABCB1 and ABCC1 transporters are crucial for a successful fertilization. Additional studies must be performed to investigate the involvement of membrane lipid homeostasis in the fertilization process.

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Section: Discussionmentioning

confidence: 99%

Involvement of ABCB1 and ABCC1 transporters in sea urchin Echinometra lucunter fertilization

Silva-Neta¹,

Torrezan²,

Leite³

et al. 2012

Molecular Reproduction Devel

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show abstract

“…The pattern of the Ca 2+ changes in the oocyte fertilized by sperm is different in each species. However, the studies of the intracellular Ca 2+ change in many species have shown that it signals re-initiation of meiosis or embryonic development [28][29][30][31][32][33][34][35][36][37][38]. Artificial induction of Ca 2+ increase in the oocyte induced parthenogenetic activation of the oocytes without sperm [39,40].…”

Section: Plural Ca 2+ Changes Proceed In An Oocyte During Fertilizationmentioning

confidence: 98%

Intracellular calcium signaling in the fertilized eggs of Annelida

Nakano¹,

Deguchi²,

Kyozuka³

2014

Biochemical and Biophysical Research Communications

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Extracellular Ca2+ influx is crucial for the early embryonic development of the sea urchin Echinometra lucunter

Cited by 2 publications

References 47 publications

Involvement of ABCB1 and ABCC1 transporters in sea urchin Echinometra lucunter fertilization

Involvement of ABCB1 and ABCC1 transporters in sea urchin Echinometra lucunter fertilization

Intracellular calcium signaling in the fertilized eggs of Annelida

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