Extracellular Adenosine Triphosphate Stimulates Acrosomal Exocytosis in Bovine Spermatozoa via P2 Purinoceptor1

Luria, A. R.; Rubinstein, Sara; Lax, Yehudit; Breitbart, Haim

doi:10.1095/biolreprod66.2.429

Cited by 50 publications

(46 citation statements)

References 56 publications

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“…In fact, our earlier study in pancreatic duct cells has showed that NYD-SP27 acts as a physical PLC inhibitor [7]. In this study, Ca 2+ mobilization in sperm induced by either extracellular ATP or progesterone, whose receptors are known to be coupled to PLC [13,[21][22][23], was inhibited by both PLC inhibitor and retention of mNYD-SP27 in sperm by its antiserum. Taken together, these observations show that capacitation is made possible only if NYD-SP27, an intrinsic decapacitation factor, is removed from sperm.…”

Section: Discussionmentioning

confidence: 72%

“…ATP and progesterone have been considered physiological agonists of the PI-PLC pathway [13,21,22] and can induce the acrosome reaction in vitro through different pathways and mechanisms. Our study indeed shows that although NYD-SP27 was retained in sperm, ATP and progesterone show differential effects on the number of acrosome-reacted sperm (Figures 7 and 8).…”

Section: Discussionmentioning

confidence: 99%

“…ATP activates PLC through binding to the P 2y receptor on the sperm membrane, which is coupled to a G protein. This leads to increased intracellular Ca 2+ and activation of PKC, and finally, the acrosome reaction [13]. On the other hand, progesterone induces the acrosome reaction by binding to its receptor, leading to activation of PLC and PLA 2 simultaneously [22,23], and the progesterone-induced acrosome reaction can be prevented only when both PLC and PLA 2 inhibitors are present.…”

Section: Discussionmentioning

confidence: 99%

“…Extracellular ATP can stimulate the acrosome reaction in bovine spermatozoa through the P 2 purinoceptor, and its coupling to the PLC pathway is well known [13]. On the other hand, the anti-NYD-SP27 serum had less of an effect on the progesteroneinduced acrosome reaction (Figure 8), which is mediated by both the PLC and the phospholipase A 2 (PLA 2 ) signalling pathways.…”

Section: Inhibitory Effects Of Nyd-sp27 and Plc Inhibition On Capacitmentioning

confidence: 99%

“…To induce the acrosome reaction, progesterone (prepared in dimethylsulphoxide; Sigma) or extracellular ATP (prepared in normal saline) was added directly to the culture medium containing either the antiserum or the PLC inhibitor to a final concentration of 15 mmol L -1 [12] or 2.5 mmol L -1 [13], and incubated for an additional 15 min to induce the acrosome reaction. At different time points, spermatozoa suspensions were collected for CTC staining.…”

Section: Antibody and Plc Inhibitor Treatmentmentioning

confidence: 99%

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NYD-SP27, a novel intrinsic decapacitation factor in sperm

Bi¹,

Xu²,

Wong³

et al. 2009

Asian J Androl

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Abstract:Prior to fertilization sperm has to undergo an activation process known as capaciation, leading to the acrosome reaction. Till now, little is known about the mechanism for preventing premature capacitation in sperm although decapacitation factors from various sources have been thought to be involved. In this study, we report that NYD-SP27, an isoform of phospholipase C Zeta 1 (PLCZ1), is localized to the sperm acrosome in mouse and human spermatozoa by immunofluorescence using a specific antibody. Western blot and double staining analyses show NYD-SP27 becomes detached from sperm, as they undergo capacitation and acrosome reaction. The absence of HCO 3 -, a key factor in activating capacitation, from the capacitation-inducing medium prevents the loss of NYD-SP27 from sperm. The anti-NYD-SP27 antibody also prevents the loss of NYD-SP27 from sperm, reduced the number of capacitated sperm, inhibited the acrosome reaction induced by ATP and progesterone, and inhibited agonist-induced PLC-coupled Ca 2+ mobilization in sperm, which can be mimicked by the PLC inhibitor, U73122. These data strongly suggest that NYD-SP27 is a physiological inhibitor of PLC that acts as an intrinsic decapacitation factor in sperm to prevent premature capacitation and acrosome reaction.

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Section: Discussionmentioning

confidence: 72%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Inhibitory Effects Of Nyd-sp27 and Plc Inhibition On Capacitmentioning

confidence: 99%

Section: Antibody and Plc Inhibitor Treatmentmentioning

confidence: 99%

See 3 more Smart Citations

NYD-SP27, a novel intrinsic decapacitation factor in sperm

Bi¹,

Xu²,

Wong³

et al. 2009

Asian J Androl

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Involvement of a P2X7 Receptor in the Acrosome Reaction Induced by ATP in Rat Spermatozoa

Torres-Fuentes

Ríos

Moreno

2015

Journal Cellular Physiology

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The acrosome reaction (AR) is the exocytosis of the acrosomal vesicle in response to different physiological and non-physiological stimuli. Particularly in mammals, the AR is needed for sperm to fuse with the oocyte plasma membrane, and it occurs only in capacitated sperm. Previous evidence in the literature indicates that extracellular ATP induces the AR in capacitated human and bovine spermatozoa, but its receptor has not yet been identified. The aim of this work was to define a putative ATP receptor in rat spermatozoa using pharmacological and biochemical approaches. We found that ATP induced the AR only in capacitated rat spermatozoa, which was inhibited in the presence of two general inhibitors of ATP receptors (P2 receptors), Suramin, and oxidized ATP (oATP), and one inhibitor of P2X receptor (pyridoxalphosphate-6-azophenyl-2',4'-disulfonic acid [PPADS]). In addition, the AR induced by ATP in capacitated rat spermatozoa was inhibited by brilliant blue-G (BB-G) and 17-β-oestradiol, two blockers of P2X7 receptors. Moreover, the ATP analog 2'(3')-O-(4-benzoylbenzoyl) ATP (BzATP) was almost 500 times more potent than ATP to induce the AR, which agrees with the pharmacology of a P2X7 receptor. Here, we show the presence of P2X7 receptor by Western blot and its localization in the tail and acrosome by indirect immunofluorescence. Finally, we quantify the presence of ATP in the rat oviduct during the estrous cycle. We found that the ATP concentration within the lumen of the oviduct is similar to those required to induce acrosome reaction, which agree with its role during in vivo fertilization. Therefore, our results strongly suggest that ATP induces the AR in capacitated rat spermatozoa through a P2X7 receptor, which may be functional during in vivo fertilization.

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Prostasomes: Their Characterisation: Implications for Human Reproduction

Ronquist

2015

Advances in Experimental Medicine and Biology

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The prostate is a principal accessory genital gland that is vital for normal fertility. Epithelial cells lining the prostate acini release in a defined fashion (exocytosis) organellar nanosized structures named prostasomes. They are involved in the protection of sperm cells against immune response in the female reproductive tract by modulating the complement system and by inhibiting monocyte and neutrophil phagocytosis and lymphocyte proliferation. The immunomodulatory function most probably involves small non-coding RNAs present in prostasomes. Prostasomes have also been proposed to regulate the timing of sperm cell capacitation and induction of the acrosome reaction, since they are rich in various transferable bioactive molecules (e.g. receptors and enzymes) that promote the fertilising ability of sperm cells. Antigenicity of sperm cells has been well documented and implicated in involuntary immunological infertility of human couples, and antisperm antibodies (ASA) occur in several body fluids. The propensity of sperm cells to carry attached prostasomes suggests that they are a new category of sperm antigens. Circulating human ASA recognise prostasomes, and among 12 identified prostasomal antigens, prolactin- inducible protein (95 %) and clusterin (85 %) were immunodominant at the expense of the other 10 that were sporadically occurring.

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Extracellular Adenosine Triphosphate Stimulates Acrosomal Exocytosis in Bovine Spermatozoa via P2 Purinoceptor1

Cited by 50 publications

References 56 publications

NYD-SP27, a novel intrinsic decapacitation factor in sperm

NYD-SP27, a novel intrinsic decapacitation factor in sperm

Involvement of a P2X7 Receptor in the Acrosome Reaction Induced by ATP in Rat Spermatozoa

Prostasomes: Their Characterisation: Implications for Human Reproduction

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