Extração de ecdisterona em raízes de ginseng brasileiro

Abstract

Este estudo teve como objetivo otimizar a extração de ecdisterona em raízes de ginseng brasileiro. Primeiramente, para se avaliar a eficiência do solvente extrator, amostras de raízes dois acessos (BRA e JB-UFSM) de P. glomerata foram extraídas em Soxhlet com metanol e clorofórmio, separadamente, durante 4 horas. No segundo ensaio, com o intuito de se escolher o método extrator, a extração foi conduzida em Soxhlet e em ultrassom, utilizando metanol como solvente. Em P. tuberosa, as amostras foram extraídas com metanol, e a extração foi conduzida em Soxhlet e em banho ultrasônico. O conteúdo de ecdisterona foi determinado em Cromatógrafo Líquido de Alta Eficiência (CLAE). Em ambas as espécies, um maior conteúdo de ecdisterona foi detectado nas amostras extraídas com metanol e em Soxhlet. A metodologia proposta mostrou-se eficaz para a quantificação da ecdisterona a partir das raízes de P. glomerata e P. tuberosa, podendo ser aplicada no controle de qualidade de drogas vegetais e/ou fitoterápicos.

Quantification of β-ecdysone in differents parts of Pfaffia glomerata by HPLC

“…Crude extract were obtained from five methods of extraction: maceration (Neto et al, 2005) percolation (Farmacopéia Brasileira, 1988) turbolyse (De-Paris et al, 2000) reflux (Gosmann et al, 2003) and Soxhlet method (Flores et al, 2009) using three different mixture of solvents, ethanol:water (90:10 v/v), (80:20 v/v) and (50:50 v/v). For the preparation of extracts, used 10 g of P. glomerata triturated and the extract was filtered, the organic solvent was removed under "vacuum" and lyophilized.…”

Micropropagation and β-ecdysone content of the Brazilian ginsengs Pfaffia glomerata and Pfaffia tuberosa

“…Plants of both P. glomerata genotypes showed a high survival rate during the acclimatization phase (Table 2) and a good adaptation to the environmental conditions over the 2 yr of field culture. In a recent study, Flores et al (2009a) studied the impact of two cytokinins (benzylaminopurine and thidiazuron) on the micropropagation of P. glomerata and observed that the addition of these growth regulators was not effective for this species. Thus, the multiplication of Brazilian ginseng by means of nodal segments on MS medium without growth regulators (Nicoloso et al 2001) has proven to be a suitable method to obtain a large number of homogeneous plants with high phenotypical quality.…”

“…Stem segments (about 5 cm long) of P. glomerata and P. tuberosa isolated from fieldgrown plants were disinfected by: immersion in 70% (v/v) ethanol solution for 10 s; followed by 10 min immersion, with agitation, in 1.0% sodium hypochlorite (v/v) containing two drops of commercial detergent per 100 ml solution; and lastly, in 0.1% (w/v) HgCl 2 solutions for 5 min, and then rinsed five times in sterile, distilled water. Disinfected nodal segments (1 cm long microcuttings) were transferred into glass culture tubes (25-150 mm) containing 10 ml of the solidified medium of Murashige and Skoog (MS) (Murashige and Skoog, 1962), supplemented with 100 mg l −1 myo-inositol, 30 gl −1 sucrose, and 6 gl −1 agar (Merck), according to Nicoloso et al (2001) for P. glomerata and Flores et al (2006) for P. tuberosa. The pH was adjusted to 5.9, and the medium was autoclaved at 121°C and 1.15 kg cm −2 for 20 min.…”

“…Nodal segments (1 cm long) from in vitro grown plants of both genotypes (BRA and JB-UFSM) were micropropagated in hormone-free MS-based medium according to Nicoloso et al (2001) and Flores et al (2009a). Percentage of explants that produced shoots, number of shoots per explant, number of nodal segments per explant, shoot length, and percentage of rooting were assessed after 4 wk of culture.…”

“…Consequently, a tissue culture technique might play an important role in the propagation of Brazilian ginsengs. We have previously reported the production of plants using nodal segments of P. glomerata (Nicoloso et al 2001;Flores et al 2009a) and P. tuberosa (Flores et al 2006;Flores et al 2007). However, to our knowledge, there are no reports in the literature about β-ecdysone production from micropropagated plants of these species.…”