Quantification of β-ecdysone in differents parts of Pfaffia glomerata by HPLC

Abstract

Pfaffi a glomerata (Spreng.) Pedersen, Amaranthaceae, is widely distributed in Brazil. Roots are considered as the world's greatest supplier and β-ecdysone is the most important compound extracted from roots of Pfaffi a glomerata. So, the aim this study was analyze the presence of β-ecdysone in the infl orescences and stems and compared with the content from roots of Pfaffi a glomerata and determine the best extractive method of β-ecdysone this plant. The crude extracts were obtained by Soxhlet method, refl ux, maceration, percolation and turbolyse. Compound extracts were quantifi ed by High Performance Liquid Chromatography (HPLC). The analysis were carried out a Phenomenex Column C18, 5 µm, 250x4,6mm, maintened at 30 o C, gradient system using as mobile phase a mixture of methanol and water, fl ow rate 1,0 mL and detection at 245 nm. Results showed Soxhlet method with ethanol:water (90:10 v/v) presented the higher concentration of β-ecdysone in P. glomerata and infl orescences showed higher amount of this active substance (3,06%), compared with stems (2,37%) and roots (1,63%), showing that the infl orescences and plant stems may also be used as a rich source of β-ecdysone.

Phytochemical Analysis of Pfaffia glomerata Inflorescences by LC-ESI-MS/MS

“…The extract obtained from the inflorescences was prepared using the Soxhlet method, with 10 g of plant material and a solution of ethanol:water (9:1 v/v) as established by Serra et al . [13]. The extract was filtered, and the organic solvent was removed under vacuum at 40 °C, using a rotary evaporator, and lyophilized.…”

“…The β-ecdysone content in P. glomerata has been analyzed almost exclusively in roots, which contain large amounts of this compound [5,7,12]. However, recent studies have confirmed the presence of significant amounts β-ecdysone in all of the major parts of P. glomerata , including stems and inflorescences [3,13]. Many pharmacological effects are attributed to β-ecdysone, such as antioxidant activity, and wound-healing and skin-regenerating properties, in addition to its use in cosmetic preparations [6,14,15,16].…”

Isolation and prebiotic activity of inulin-type fructan extracted from Pfaffia glomerata (Spreng) Pedersen roots

“…This plant is popularly known as "Brazilian ginseng," and it is used commercially as a substitute for Asian ginseng, Panax ginseng. P. glomerata roots are used in folk medicine as a tonic, aphrodisiac, anti-diabetic, and antiulcer gastric, with a number of studies demonstrating its effectiveness [1][2][3][4][5][6].…”

“…To date, the main components isolated from P. glomerata roots are glomeric acid (triterpenoid), pfameric acid (nortriterpenoid), rubrosterone, and β-ecdysone [3,7]. Among the active ingredients contained in this plant, the steroid β-ecdysone is the most important compound extracted from the roots [8].…”

β-ecdysone content and antioxidant capacity in different organs of Brazilian ginseng

“…β-ecdysone (20-hydroxyecdysone) is a relevant marker compound in the extractive solution from roots of P. glomerata (ZIMMER et al, 2006). Previous studies have confirmed the presence of β-ecdysone in all of the major organs of P. glomerata, such as roots (FREITAS et al, 2004;LEAL et al, 2010), stems, and inflorescences (SERRA et al, 2012). β-ecdysone is a secondary metabolite with biotechnological, pharmacological, medical, and agrochemical applicability (FELIPE et al, 2019).…”