EXTRAÇÃO DE DNA GENÔMICO DE Passiflora spp. PARA ANÁLISES PCR-RAPD

Molinari, Hugo Bruno Correa; Crochemore, Maria Lúcia

doi:10.1590/s0100-29452001000200051

Cited by 13 publications

(12 citation statements)

References 7 publications

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“…Leaves of two plants from each accession were collected to extract the genomic DNA by the CTAB method according to Molinari & Crochemore (2001). Fresh plant tissue was macerated with a glass bar.…”

Section: Methodsmentioning

confidence: 99%

Genetic variability of hull-less barley accessions based on molecular and quantitative data

Sayd

Amábile

Faleiro

et al. 2015

Pesq. agropec. bras.

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-The objective of this work was to characterize and quantify the genetic, molecular, and agronomic variability of hull-less barley genotypes, for the selection of parents and identification of genotypes adapted to the irrigated production system in the Brazilian Cerrado. Eighteen hull-less barley accessions were evaluated, and three covered barley accessions served as reference. The characterization was based on 157 RAPD molecular markers and ten agronomic traits. Genetic distance matrices were obtained based on molecular markers and quantitative traits. Graphic grouping and dispersion analyses were performed. Genetic, molecular, and agronomic variability was high among genotypes. Ethiopian accessions were genetically more similar, and the Brazilian ones were genetically more distant. For agronomic traits, two more consistent groupings were obtained, one with the most two-rowed materials, and the other with six-rowed materials. The more diverging materials were the two-rowed CI 13453, CN Cerrado 5, CN Cerrado 1, and CN Cerrado 2. The PI 356466, CN Cerrado 1, PI 370799, and CI 13453 genotypes show agronomic traits of interest and, as genetically different genotypes, they are indicated for crossing, in breeding programs.Index terms: Hordeum vulgare, genetic diversity, molecular markers, quantitative traits. Variabilidade genética de acessos de cevada-nua com base em dados moleculares e quantitativosResumo -O objetivo deste trabalho foi caracterizar e quantificar a variabilidade genética, molecular e agronômica de genótipos de cevada-nua, para a seleção de genitores e a identificação de genótipos adaptados ao sistema de produção irrigada no bioma Cerrado. Dezoito acessos de cevada-nua foram avaliados e três acessos de grãos de cevada com casca serviram como referência. A caracterização baseou-se em 157 marcadores moleculares RAPD e dez características agronômicas. As matrizes de distância genética foram obtidas com base em marcadores moleculares e características quantitativas. Realizaram-se análises de agrupamento e dispersão gráfica dos acessos. A variabilidade genética, molecular e agronômica foi alta entre os acessos. Os acessos etíopes foram geneticamente mais similares, e os acessos brasileiros, geneticamente mais distantes. Quanto às características agronômicas, obtiveram-se dois agrupamentos mais consistentes, um com mais materiais dísticos e outro com os hexásticos. Os genótipos mais divergentes foram os dísticos CI 13453, CN Cerrado 5, CN Cerrado 1 e CN Cerrado 2. Os genótipos PI 356466, CN Cerrado 1, PI 370799 e CI 13453 apresentam características agronômicas de interesse e, como são distintos geneticamente, são indicados para cruzamentos em programas de melhoramento genético.Termos para indexação: Hordeum vulgare, diversidade genética, marcador molecular, caracteres quantitativos.

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Section: Methodsmentioning

confidence: 99%

Genetic variability of hull-less barley accessions based on molecular and quantitative data

Sayd

Amábile

Faleiro

et al. 2015

Pesq. agropec. bras.

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“…The leaves were oven-dried (37ºC) for 12 h and then macerated until a fine dust. DNA extraction was performed as described by Molinari and Crochemore (2001). DNA concentration was estimated in a fluorimeter as described by the manufacturer (Hoefer Scientific Instruments).…”

Section: Dna Extractionmentioning

confidence: 99%

Genetic diversity in passion fruit (Passiflora spp.) evaluated by RAPD markers

Crochemore

Molinari

Vieira

2003

Braz. arch. biol. technol.

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“…Moreover, the method is less laborious and safer, since 2-mercaptoethanol was eliminated from this protocol. 2-Mercaptoethanol is volatile and toxic and requires additional care when handled (Molinari and Crochemore, 2001;Sambrook and Russel, 2001). …”

Section: Resultsmentioning

confidence: 99%

“…Most of all, the impurities can interfere in the action of restriction enzymes, Taq polymerase in PCR reactions or electrophoretic separation of DNA fragments (Molinari and Crochemore, 2001). An efficient protocol for DNA isolation provides good conditions for PCR amplification allowing reproducibility and clear bands.…”

Section: Introductionmentioning

confidence: 99%

Genomic DNA isolation of Acrocomia aculeata (Arecaceae) from leaf and stipe tissue samples for PCR analysis

Lanes¹,

Nick²,

Kuki³

et al. 2013

Genet. Mol. Res.

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ABSTRACT. Macaw palm, Acrocomia aculeata is an oleaginous species of the Arecaceae family; it has been identified as one of the most promising plants for sustainable production of renewable energy, especially biodiesel. We developed an efficient protocol of genomic DNA extraction for A. aculeata using leaf and stipe tissues, based on the cationic hexadecyltrimethylammonium bromide method, and we evaluated the quantity, purity, and integrity of the resultant DNA. We also determined whether these procedures interfere with PCR amplification using SSR molecular markers. The lowest concentration of DNA was obtained from stipe tissues (135 ng/µL), while fresh leaf tissues provided the highest concentration of DNA (650 ng/µL). Good quality DNA was obtained from fresh leaf, lyophilized leaf, and stipe tissues (relative purity, 1.79-1.89 nm). Differences in quantity and quality of DNA extracted from different tissues did not interfere with general patterns of PCR amplification based on SSR markers.

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EXTRAÇÃO DE DNA GENÔMICO DE Passiflora spp. PARA ANÁLISES PCR-RAPD

Cited by 13 publications

References 7 publications

Genetic variability of hull-less barley accessions based on molecular and quantitative data

Genetic variability of hull-less barley accessions based on molecular and quantitative data

Genetic diversity in passion fruit (Passiflora spp.) evaluated by RAPD markers

Genomic DNA isolation of Acrocomia aculeata (Arecaceae) from leaf and stipe tissue samples for PCR analysis

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