External Quality Assurance programs for processing methods provide evidence on impact of preanalytical variables

Verderio, Paolo; Ciniselli, Chiara Maura; Gaignaux, Amélie; Pastori, Marta; Saracino, Sabrina; Kofanova, Olga; Betsou, Fay

doi:10.1016/j.nbt.2022.08.006

Cited by 6 publications

(3 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Participation in a Proficiency Testing scheme would be beneficial, because these enable a laboratory to compare their processes with their peers, using the same starting material [ [15] , [16] , [17] ]. Consequently, a report advising a laboratory that their processes yield nucleic acids that are unusually degraded suggests there could be a nuclease contamination issue.…”

Section: Discussionmentioning

confidence: 99%

Implementing routine monitoring for nuclease contamination of equipment and consumables into the quality Management system of a laboratory

Henry,

Charalambous,

Betsou

et al. 2024

Heliyon

Self Cite

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 99%

Implementing routine monitoring for nuclease contamination of equipment and consumables into the quality Management system of a laboratory

Henry,

Charalambous,

Betsou

et al. 2024

Heliyon

Self Cite

View full text Add to dashboard Cite

“…These measures encompass the utilization of standard operating procedures (SOPs) and validated protocols, incorporating internal quality controls such as reference materials and control charts, active participation in PT programs, and obtaining certification or accreditation according to recognized standards such as ISO15189/IEC, ISO/IEC 17025 and ISO 9001 (6). Indeed, it has been demonstrated that laboratories engaged in PT programs exhibit robust internal quality control procedures and consistently achieve better z-scores, (7, 8).…”

Section: Proficiency Testing For Assessing Pre-analytical Sample Proc...mentioning

confidence: 99%

Multi-omics Quality Assessment in Personalized Medicine through EATRIS

Alonso-Andrés,

Baldazzi,

Chen

et al. 2023

Preprint

Self Cite

View full text Add to dashboard Cite

Molecular characterization of a biological sample, e.g., with omics approaches, is fundamental for the development and implementation of personalized and precision medicine approaches. In this context, quality assessment is one of the most critical aspects. Accurate performance and interpretation of omics techniques is based on consensus, harmonization, and standardization of protocols, procedures, data analysis and reference values and materials. EATRIS, the European Infrastructure for Translational Medicine (www.EATRIS.eu), brings together resources and services to support researchers in developing their biomedical discoveries into novel translational tools and interventions for better health outcomes. Here we describe activities of member facilities of EATRIS towards quality assessment of pre-clinical sample processing, clinical omics data generation, multi-omics data integration, and dissemination of the resources in a Multi-Omics Toolbox, the principal deliverable of the EATRIS Plus project for the consolidation of EATRIS towards translational Medicine.

show abstract

“…As such, DNA sample management standardization is useful to obtain DNA of quality, even if genomic DNA appears less sensitive than RNA to factors affecting biospecimen quality [17]. A recent study conducted in the frame of External Quality Assurance (EAQ) program reports that DNA yields are impacted by extraction method and elution buffer, and DNA integrity by extraction method and equipment [18]. Additionally, we performed a retrospective study to analyze the impact of preanalytical factors and patient characteristics (age, sex and BMI) on the quantity of DNA extracted.…”

Section: Tissue Typementioning

confidence: 99%

Addressing the quality challenge of a human biospecimen biobank through the creation of a quality management system

et al. 2022

View full text Add to dashboard Cite

Background The objective of the COMET (COllection of MEtabolic Tissues) biobank project is to create a high-quality collection of insulin-sensitive tissues (liver, muscle, adipose tissues, and epiploic artery) and blood sample derivatives (plasma, serum, DNA and RNA), collected from 270 grade 2–3 obese patients undergoing bariatric surgery. Relevant data on patient such as clinical/biological characteristics and sample handling are also collected. For this, our aim was to establish a Quality Management System (QMS) to meet the reliability and quality requirements necessary for its scientific exploitation. Materials and methods The COMET QMS includes: (1) Quality Assurance to standardize all stages of the biobanking process, (2) Quality Controls on samples from the first patients included in order to validate the sample management process and ensure reproducible quality; and 3) “in process” Quality Controls to ensure the reliability of the storage procedures and the stability of the samples over time. Results For serum and plasma, several corrective actions, such as temperature handling and centrifugation conditions, were made to the protocol and led to improvement of the volume and quality of samples. Regarding DNA, all samples evaluated achieved a satisfactory level of purity and integrity and most of them yielded the required DNA quantity. All frozen tissue samples had RNAs of good purity. RNA quality was confirmed by RIN, achieving values in most cases over 7 and efficient amplification of housekeeping genes by RT-qPCR, with no significant differences among samples from the same tissue type. In the “in process” Quality Controls, DNA, RNA, and histological integrity of tissues showed no differences among samples after different preservation times. Conclusion Quality Control results have made it possible to validate the entire biobank process and confirm the utility of implementing QMS to guarantee the quality of a biospecimen collection.

show abstract

External Quality Assurance programs for processing methods provide evidence on impact of preanalytical variables

Cited by 6 publications

References 26 publications

Implementing routine monitoring for nuclease contamination of equipment and consumables into the quality Management system of a laboratory

Implementing routine monitoring for nuclease contamination of equipment and consumables into the quality Management system of a laboratory

Multi-omics Quality Assessment in Personalized Medicine through EATRIS

Addressing the quality challenge of a human biospecimen biobank through the creation of a quality management system

Contact Info

Product

Resources

About