Multi-omics Quality Assessment in Personalized Medicine through EATRIS

Abstract

Molecular characterization of a biological sample, e.g., with omics approaches, is fundamental for the development and implementation of personalized and precision medicine approaches. In this context, quality assessment is one of the most critical aspects. Accurate performance and interpretation of omics techniques is based on consensus, harmonization, and standardization of protocols, procedures, data analysis and reference values and materials. EATRIS, the European Infrastructure for Translational Medicine (www.EATRIS.eu), brings together resources and services to support researchers in developing their biomedical discoveries into novel translational tools and interventions for better health outcomes. Here we describe activities of member facilities of EATRIS towards quality assessment of pre-clinical sample processing, clinical omics data generation, multi-omics data integration, and dissemination of the resources in a Multi-Omics Toolbox, the principal deliverable of the EATRIS Plus project for the consolidation of EATRIS towards translational Medicine.

Elevated fecal calprotectin is associated with gut microbial dysbiosis, altered serum markers and clinical outcomes in older individuals

“…Serum metabolites were relatively quantified using targeted metabolomics of 460 metabolites (135 detected) based on validated 60 liquid chromatography–mass spectrometry (LC–MS) methods. Briefly, metabolites were extracted from 100 µl serum with 400 µl of extraction solution (ACN:MeOH:MQ) centrifuged.…”

Elevated fecal calprotectin is associated with gut microbial dysbiosis, altered serum markers and clinical outcomes in older individuals

“…Serum metabolites were relatively quanti ed using targeted metabolomics of 460 metabolites (135 detected) based on validated 59 Vanquish UHPLC (Thermo Fischer Scienti c) coupled with Q-Exactive Orbitrap mass spectrometer (Thermo Fischer Scienti c). Separation was performed at ow rate of 0.100 ml/minutes with SeQuant ZIC-pHILIC column (Merck) having gradient within 24 min (from 80% B to 20% B), using acetonitrile as mobile phase B and 20mM ammonium hydrogen carbonate, pH 9.4, as mobile phase A.…”