External Egg Membrane Characteristics of Landlocked Fall Chinook Salmon Eyed Eggs Treated with Hydrogen Peroxide or Formalin

Stephenson, Hans; Gabel, Mark; Barnes, Michael E.

doi:10.1577/a04-042.1

Cited by 3 publications

(2 citation statements)

References 19 publications

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“…However, if eggs were treated the day before hatching, significant mortality occurred. Stephenson et al (2005) observed in Chinook salmon Oncorhynchus tshawytscha that the internus layer of egg shells treated with hydrogen peroxide (700 mg/L daily) was significantly less differentiated than that in eggs treated with formalin or those left untreated. Iodine has also been reported to induce higher mortality and premature hatching if applied near hatching (Piper et al 1982).…”

Section: Discussionmentioning

confidence: 93%

Laboratory and Production Scale Disinfection of Salmonid Eggs with Hydrogen Peroxide

Wagner¹,

Oplinger²,

Bartley³

2012

N American J Aquac

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Disinfection tests were conducted on eggs from rainbow trout Oncorhynchus mykiss, cutthroat trout O. clarkii, and brown trout Salmo trutta to evaluate hydrogen peroxide (H 2 O 2 ) as an egg disinfectant. A daily drip of 500 mg/L hydrogen peroxide for 35 min on eyed brown trout eggs safely led to significantly reduced bacterial abundance relative to untreated controls, but abundance did not differ significantly from that in a formalin treatment (2,000 mg/L for 15 min). Using water-hardened cutthroat trout eggs, hydrogen peroxide concentrations of (1) 10 g/L for 2 min, (2) 10 g/L for 3 min, (3) 15 g/L for 2 min, and (4) 1,000 mg/L for 15 min were compared with two controls (untreated; 100 mg/L iodine). Bacteria were significantly more abundant in the untreated eggs, but abundance did not significantly differ among chemical treatments. In another test, rainbow trout eggs treated the day before hatch with 15 g/L H 2 O 2 for 2 min had significantly higher mortality than the controls. In production-scale tests, 10-15 g/L H 2 O 2 for 2 min was safe for rainbow and cutthroat trout eggs and significantly reduced bacterial abundance relative to that in untreated eggs. Complete disinfection was not achievable with either iodine or hydrogen peroxide. Daily drip treatment with either formalin or hydrogen peroxide significantly reduced bacterial growth and is recommended.

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Section: Discussionmentioning

confidence: 93%

Laboratory and Production Scale Disinfection of Salmonid Eggs with Hydrogen Peroxide

Wagner¹,

Oplinger²,

Bartley³

2012

N American J Aquac

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“…It is also important to note that disinfection with H 2 O 2 can cause changes in the egg morphology (Stephenson et al . ). These changes could interfere with hatching or normal embryonic development.…”

Section: Evaluation Criteria For a Successful Disinfectionmentioning

confidence: 97%

Disinfection of teleost eggs: a review

Swaef

Broeck

Dierckens

et al. 2015

Reviews in Aquaculture

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In aquaculture hatcheries, egg disinfection is commonly employed as a mortality mitigation and disease management tool. In addition, disinfection protocols are utilized in research facilities, as a means to create axenic and gnotobiotic larval models. For the latter, a complete sterilization of the eggs is warranted. Multiple research groups have tested various disinfectants in fish eggs adopting different protocols and a range of parameters to evaluate their efficacy and safety. However, there is a clear lack of a critical review listing these different studies and stressing the advantages and shortcomings of the protocols and adopted disinfectants. This review provides a commented survey of the findings/experiments on the most frequently used and upcoming disinfecting products, the protocols in which they are included and their purposes with emphasis on decreasing bacterial and/or fungal load, underscoring the benefits and the limitations of each product. Supplemented with a critical note, this review assists both aquaculturists and researchers in making an informed choice. Moreover, it will help stakeholders to identify the gaps in knowledge where future research on egg disinfection is needed.

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Use of Hydrogen Peroxide to Improve Golden Shiner Egg Hatchability

2009

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The efficacy of hydrogen peroxide (H2O2) in controlling fungal infections (saprolegniasis) in the eggs of golden shiner Notemigonus crysoleucas was assessed in two experiments. Golden shiner eggs were exposed in a 15‐min static bath (21°C) to 0, 50, 100, or 200 mg H2O2/L in the first experiment and to 0, 200, 400, and or 800 mg/L in the second experiment. All treatments were based on the amount of active ingredient (30% active ingredient concentration of H2O2) in a single treatment. Three replicates of each concentration were used in each experiment. The objective was to determine the H2O2 concentration that would result in optimum hatching success. The hatching rate significantly increased in each treatment level until 800 mg/L. The mean ± SE percent egg hatchability was 72.3 ± 8.55% and 68.2 ± 5.03% at 400 and 800 mg/L, respectively. Regression analysis revealed the peak treatment level to be between 400 and 800 mg/L.

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External Egg Membrane Characteristics of Landlocked Fall Chinook Salmon Eyed Eggs Treated with Hydrogen Peroxide or Formalin

Cited by 3 publications

References 19 publications

Laboratory and Production Scale Disinfection of Salmonid Eggs with Hydrogen Peroxide

Laboratory and Production Scale Disinfection of Salmonid Eggs with Hydrogen Peroxide

Disinfection of teleost eggs: a review

Use of Hydrogen Peroxide to Improve Golden Shiner Egg Hatchability

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