Extensive Phytochemical Assessment of Dendrobium fimbriatum Hook (Orchidaceae)

Abstract

Dendrobium fimbriatum Hook is an important medicinal and ornamental orchid that may lead to important drugs and cosmetics. In this study, 2 analytical strategies were combined to describe the detailed composition of a hydroethanolic extract of the whole plant. The CARAMEL approach, based on centrifugal partition chromatography and nuclear magnetic resonance data interpretation, allowed the rapid dereplication of 21 major constituents, mainly including polyols, nucleosides, zwitterionic compounds, phenolic compounds, and organic acids. Further semipreparative high-performance liquid chromatography and spectroscopic analyses led to the isolation of 6 additional compounds; 4 phenanthrenes (plicatol B, hircinol, plicatol A, and plicatol C), 1 bibenzyl (3′,4-dihydroxy-3,5′-dimethoxybibenzyl), and 1 furostanol saponin (protodioscin), as well as a new phenanthrene derivative, plicatol D. A total of 28 metabolites were structurally elucidated, among which 23 are described for this species for the first time.

Rapid Chemical Profiling of Filipendula ulmaria Using CPC Fractionation, 2-D Mapping of 13C NMR Data, and High-Resolution LC–MS

“…One can also note that the mass quantities obtained by CPC for each fraction are sufficiently high to perform biological tests in parallel. Over the last years, this procedure has demonstrated its robustness through applications on natural extracts of various origins including terrestrial plants, microalgae and macroalgae, or cultured cell extracts of a plant or microbial species [24][25][26][27][28][29][30]. This identification process was completed by high-resolution LC-MS analysis of the crude extract.…”

Chemical profiling of botanical extracts obtained in NADES systems using centrifugal partition chromatography combined with 13C NMR dereplication—Hypericum perforatum as a case study

“…In the last years the robustness of the procedure has been reinforced through many concrete cases of phytochemical investigations conducted on natural extracts of various origins including terrestrial plants and micro-and macroalgae or cultured cell extracts of plant or microbial species. [16][17][18][19][20][21][22] Here, the chemical profile of a NADES extract obtained from the plant species Hypericum perforatum was investigated. H. perforatum L. (St. John's wort) is a medicinal plant widely used in herbal teas and dietary supplements, mainly to treat depression, anxiety, and insomnia.…”

“…This approach enables to rapidly obtain a detailed chemical profile without loss of biomass, which means that the starting extract mass can be recovered at the end of the identification process in the form of a well‐characterized fraction series. In the last years the robustness of the procedure has been reinforced through many concrete cases of phytochemical investigations conducted on natural extracts of various origins including terrestrial plants and micro‐ and macroalgae or cultured cell extracts of plant or microbial species 16–22 …”