Extensive macrosynteny between Medicago truncatula and Lens culinaris ssp. culinaris

Abstract

The first predominantly gene-based genetic linkage map of lentil (Lens culinaris ssp. culinaris) was constructed using an F5 population developed from a cross between the cultivars Digger (ILL5722) and Northfield (ILL5588) using 79 intron-targeted amplified polymorphic (ITAP) and 18 genomic simple sequence repeat (SSR) markers. Linkage analysis revealed seven linkage groups (LGs) comprised of 5-25 markers that varied in length from 80.2 to 274.6 cM. The genome map spanned a total length of 928.4 cM. Clear evidence of a simple and direct macrosyntenic relationship between lentil and Medicago truncatula was observed. Sixty-six out of the 71 gene-based markers, which were previously assigned to M. truncatula genetic and physical maps, were found in regions syntenic between the Lens c. ssp. culinaris and M. truncatula genomes. However, there was evidence of moderate chromosomal rearrangements which may account for the difference in chromosome numbers between these two legume species. Eighteen common SSR markers were used to connect the current map with the most comprehensive and recent map that exists for lentil, providing the syntenic context of four important domestication traits. The composite map presented, anchored with orthologous markers mapped in M. truncatula, provides a strong foundation for the future use of genomic and genetic information in lentil genetic analysis and breeding.

Legume Crops Phylogeny and Genetic Diversity for Science and Breeding

“…This was a successful strategy because of the synteny between lentil and the model legume Medicago truncatula (Phan et al, 2007b). With the publication of three transcriptomes of lentil Verma et al, 2013;Kaur et al 2011), this is rapidly changing.…”

Genetic mapping and annotation of genomic microsatellites isolated from globe artichoke

“…As previously performed in the Solanaceae (Wu et al 2006(Wu et al , 2009, Fabaceae (Phan et al 2007;Hougaard et al 2008;Ellwood et al 2008) and Asteraceae (Chapman et al 2007) families, a COS marker approach may represent an effective mean for generating molecular markers. Our comparative analysis amongst the Asteraceae species showed similarity values up to 100% between sequences from globe artichoke and those from the yellow starthistle (Centaurea solstitialis) or safflower (Carthamus tinctorius); accordingly, an exploration of the Asteraceae dbEST seems very promising for new microsatellite markers mining as well as for synteny studies.…”

Plant Responses to Elevated Temperatures: A Field Study on Phenological Sensitivity and Fitness Responses to Simulated Climate Warming

“…Hamwieh et al (2005) added 39 SSR and 50 AFLP markers to the map constructed by Eujayl et al (1998a) to produce a comprehensive Lens map comprising 283 genetic markers covering 715 cM. Subsequently, the first lentil map that contained 18 SSR and 79 cross genera ITAP gene-based markers was constructed using a F 5 RIL population developed from a cross between ILL5722 and ILL5588 (Phan et al, 2007 LGs covering a distance of 3847 cM has been constructed (Gupta et al, 2012b). Recently, population specific linkage maps are developed by Perez de la Vega et al (2011) and Andeden et al (2013).…”

“…The lentil genome has shown different degrees of synteny with other legume crops (Weeden et al, 1992;Simon and Muehlbauer, 1997;Phan et al, 2007;Choudhary et al, 2009). Development of PCRbased markers has improved transferability of genetic information among species through comparative genomics and has facilitated the establishment of phylogenetic relationship in plants species.…”