Extensive Cryptic Diversity in the Terrestrial Diatom Pinnularia borealis (Bacillariophyceae)

Pinseel, Eveline; Kulichová, Jana; Scharfen, Vojtěch; Urbánková, Pavla; Vijver, Bart Van de; Vyverman, Wim

doi:10.1016/j.protis.2018.10.001

Cited by 35 publications

(31 citation statements)

References 65 publications

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“…In addition, the detection of diatom diversity by light microscopy is limited as ecological differences are not necessarily mirrored by differences in morphological traits. Especially regarding within-species/genus variations, whereas several genetic approaches addressing diatom diversity on lower taxonomic levels have uncovered cryptic and hidden diversity in similar morphotypes [17][18][19]. With the advent of the metabarcoding approach combined with advancing high-throughput next-generation sequencing techniques, diatom compositions have been retrieved with various genetic markers and from different environments [20][21][22][23][24].…”

Section: Introductionmentioning

confidence: 99%

Lake-depth related pattern of genetic and morphological diatom diversity in boreal Lake Bolshoe Toko, Eastern Siberia

et al. 2020

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Large, old and heterogenous lake systems are valuable sources of biodiversity. The analysis of current spatial variability within such lakes increases our understanding of the origin and establishment of biodiversity. The environmental sensitivity and the high taxonomic richness of diatoms make them ideal organisms to investigate intra-lake variability. We investigated modern intra-lake diatom diversity in the large and old sub-arctic Lake Bolshoe Toko in Siberia. Our study uses diatom-specific metabarcoding, applying a short rbcL marker combined with next-generation sequencing and morphological identification to analyse the diatom diversity in modern sediment samples of 17 intra-lake sites. We analysed abundance-based compositional taxonomic diversity and generic phylogenetic diversity to investigate the relationship of diatom diversity changes with water depth. The two approaches show differences in taxonomic identification and alpha diversity, revealing a generally higher diversity with the genetic approach. With respect to beta diversity and ordination analyses, both approaches result in similar patterns. Water depth or related lake environmental conditions are significant factors influencing intra-lake diatom patterns, showing many significant negative correlations between alpha and beta diversity and water depth. Further, one near-shore and two lagoon lake sites characterized by low (0-10m) and medium (10-30m) water depth are unusual with unique taxonomic compositions. At deeper (>30m) water sites we identified strongest phylogenetic clustering in Aulacoseira, but generally much less in Staurosira, which supports that water depth is a strong environmental filter on the Aulacoseira communities. Our study demonstrates the utility of combining analyses of genetic and morphological as well as phylogenetic diversity to decipher compositional and generic phylogenetic patterns, which are relevant in understanding intra-lake heterogeneity as a source of biodiversity in the sub-arctic glacial Lake Bolshoe Toko.

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Section: Introductionmentioning

confidence: 99%

Lake-depth related pattern of genetic and morphological diatom diversity in boreal Lake Bolshoe Toko, Eastern Siberia

et al. 2020

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“…In general, we have to assume that similar assembly rules known from plants act on unicellular microbes such as diatoms (Heino & Soininen, ). Because it is known that morphologically similar diatoms can bear hidden genetic diversity (Pinseel et al, ), adding phylogenetic to taxonomic diversity will enable us to discern assembly rules in a more robust way (Van den Wyngaert, Most, Freimann, Ibelings, & Spaak, ).…”

Section: Introductionmentioning

confidence: 99%

Phylogenetic diversity and environment form assembly rules for Arctic diatom genera—A study on recent and ancient sedimentary DNA

Stoof‐Leichsenring

Pestryakova

Epp

et al. 2020

Journal of Biogeography

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Aim: This study investigates taxonomic and phylogenetic diversity in diatom genera to evaluate assembly rules for eukaryotic microbes across the Siberian tree line. We first analysed how phylogenetic distance relates to taxonomic richness and turnover.Second, we used relatedness indices to evaluate if environmental filtering or competition influences the assemblies in space and through time. Third, we used distancebased ordination to test which environmental variables shape diatom turnover.Location: Yakutia and Taymyria, Russia: we sampled 78 surface sediments and a sediment core, extending to 7,000 years before present, to capture the forest-tundra transition in space and time respectively.Taxon: Arctic freshwater diatoms. Methods:We applied metabarcoding to retrieve diatom diversity from surface and core sedimentary DNA. The taxonomic assignment binned sequence types (lineages) into genera and created taxonomic (abundance of lineages within different genera) and phylogenetic datasets (phylogenetic distances of lineages within different genera).Results: Contrary to our expectations, we find a unimodal relationship between phylogenetic distance and richness in diatom genera. We discern a positive relationship between phylogenetic distance and taxonomic turnover in spatially and temporally distributed diatom genera. Furthermore, we reveal positive relatedness indices in diatom genera across the spatial environmental gradient and predominantly in time slices at a single location, with very few exceptions assuming effects of competition. Distancebased ordination of taxonomic and phylogenetic turnover indicates that lake environment variables, like HCO 3 − and water depth, largely explain diatom turnover. Main conclusion:Phylogenetic and abiotic assembly rules are important in understanding the regional assembly of diatom genera across lakes in the Siberian tree line ecotone. Using a space-time approach we are able to exclude the influence of geography and elucidate that lake environmental variables primarily shape the assemblies. We conclude that some diatom genera have greater capabilities to adapt toThis is an open access article under the terms of the Creat ive Commo ns Attri bution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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“…, Škaloud and Rindi , Pinseel et al. ). Recent development of molecular tools has advanced our ability to discriminate among cryptic taxa, improving overall diversity estimates.…”

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confidence: 98%

Comparing Morphological and Molecular Estimates of Species Diversity in the Freshwater Genus Synura (Stramenopiles): A Model for Understanding Diversity of Eukaryotic Microorganisms

Škaloud

Škaloudová

Jadrná

et al. 2020

Journal of Phycology

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We performed a comparison of molecular and morphological diversity in a freshwater colonial genus Synura (Chrysophyceae, Stramenopiles), using the island of Newfoundland (Canada) as a case study. We examined the morphological species diversity in collections from 79 localities, and compared these findings to diversity based on molecular characters for 150 strains isolated from the same sites. Of 27 species or species‐level lineages identified, only one third was recorded by both molecular and morphological techniques, showing both approaches are complementary in estimating species diversity within this genus. Eight taxa, each representing young evolutionary lineages, were recovered only by sequencing of isolated colonies, whereas ten species were recovered only microscopically. Our complex investigation, involving both morphological and molecular examinations, indicates that our knowledge of Synura diversity is still poor, limited only to a few well‐studied areas. We revealed considerable cryptic diversity within the core S. petersenii and S. leptorrhabda lineages. We further resolved the phylogenetic position of two previously described taxa, S. kristiansenii and S. petersenii f. praefracta, propose species‐level status for S. petersenii f. praefracta, and describe three new species, S. vinlandica, S. fluviatilis, and S. cornuta. Our findings add to the growing body of literature detailing distribution patterns observed in the genus, ranging from cosmopolitan species, to highly restricted taxa, to species such as S. hibernica found along coastal regions on multiple continents. Finally, our study illustrates the usefulness of combining detailed morphological information with gene sequence data to examine species diversity within chrysophyte algae.

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Extensive Cryptic Diversity in the Terrestrial Diatom Pinnularia borealis (Bacillariophyceae)

Cited by 35 publications

References 65 publications

Lake-depth related pattern of genetic and morphological diatom diversity in boreal Lake Bolshoe Toko, Eastern Siberia

Lake-depth related pattern of genetic and morphological diatom diversity in boreal Lake Bolshoe Toko, Eastern Siberia

Phylogenetic diversity and environment form assembly rules for Arctic diatom genera—A study on recent and ancient sedimentary DNA

Comparing Morphological and Molecular Estimates of Species Diversity in the Freshwater Genus Synura (Stramenopiles): A Model for Understanding Diversity of Eukaryotic Microorganisms

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