Extending the scope of chiral separation of basic compounds by cyclodextrin-mediated capillary zone electrophoresis

Chiral recognition of verapamil by cyclodextrins studied with capillary electrophoresis, NMR spectroscopy, and electrospray ionization mass spectrometry

“…24 The enantioseparation of (±)-VP with native CDs was impossible in most of buffer systems used. 20,30 However, Bechet et al 21 achieved a partial enantioseparation of (±)-VP in a phosphoric acid-triethanolamine buffer at pH 3.0 in the presence of native ␤-CD. Our preliminary experiment confirmed the advantage of this buffer compared to sodium dihydrogenphosphate for the enantioseparation of (±)-VP (Fig.…”

“…[20][21][22][23][24][25][26][27][28][29][30][31][32][33][34][35][36][37] However, the migration order of the enantiomers is addressed only in few of them. Ohara et al 23 determined the migration order with TM-␤-CD to be R-(+)-before S-(−)-VP.…”

Chiral separations in capillary electrophoresis

“…The possibility to tune the resolution of chiral analytes via manipulation of the electroosmosis was also utilized in practically oriented studies. A gradient of the electric potential applied radially across the capillary wall [172], tetraalkylammonium ions added to the background electrolyte [173,174], and coating of the capillary wall with linear polyacrylamide [175] were used for the purpose.…”

Enantioseparation of β-blockers with two chiral centers by capillary electrophoresis using sulfated β-cyclodextrins

“…The analysis of nadolol led to three peaks in a 1-2-1 quantitative ratio, which was in favor of a probable separation of the cis-and trans-conformation with a comigration of two isomers of the two forms. Quang and Khaledi [26] obtained a separation in two peaks of the isomers of each pre-cited b-blocker using low concentrations of HP-b-CD in a pH 2.5 buffer with a reversed EOF. Micellar electrokinetic capillary chromatography systems have been developed at basic pH involving the association of an anionic surfactant to HP-b-CD or SBEb-CD: Aumatell and Wells [27] presented two peaks for the enantioseparation of nadolol using 1.45 mM SBE-b-CD and 20 mM sodium dodecyl sulfate [27].…”