Extending lifetime of gas-phase odor biosensor using liquid thickness control and liquid exchange

Deng, Hongchao; Mitsuno, Hidefumi; Kanzaki, Ryohei; Nakamoto, Takamichi

doi:10.1016/j.bios.2021.113887

Cited by 10 publications

(10 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A thin liquid film covering the cells which maintained the cell viability also allowed the odorant to penetrate . The liquid exchange can remove the residual odorant, thus guaranteeing the long-term repeatable biosensor responses . The liquid thickness control and liquid exchange module in Figure consisted of an impedance measurement circuit, a microcontroller (Arduino Nano, Arduino), and two syringe pumps (LPDA2750125D, Lee Company) .…”

Section: Methodsmentioning

confidence: 99%

“…The experiments about varied ligand concentrations could be found in our previous research. 15,21 Both extending the stimulation duration and increasing the liquid-phase sample concentration can increase the intensity of the ligand applied to the cells. Therefore, the effects of these two operations are similar.…”

Section: ■ Conclusionmentioning

confidence: 99%

“…15 The liquid exchange can remove the residual odorant, thus guaranteeing the longterm repeatable biosensor responses. 21 The liquid thickness control and liquid exchange module in Figure 1 consisted of an impedance measurement circuit, a microcontroller (Arduino Nano, Arduino), and two syringe pumps (LPDA2750125D, Lee Company). 21 Two electrodes were submerged into the assay buffer solution, and the impedance between the two electrodes represented the liquid layer thickness.…”

mentioning

confidence: 99%

“…During the experiment, the liquid thickness was controlled at 125 μm, and the liquid exchange speed was 5.44 μL/min. 22 Limited by the buffer solution volume in the syringe pumps, 21 the maximum experiment duration was around 3850 s, which meant 19 odor stimulations in total.…”

mentioning

confidence: 99%

“…21 The liquid thickness control and liquid exchange module in Figure 1 consisted of an impedance measurement circuit, a microcontroller (Arduino Nano, Arduino), and two syringe pumps (LPDA2750125D, Lee Company). 21 Two electrodes were submerged into the assay buffer solution, and the impedance between the two electrodes represented the liquid layer thickness. By comparing the current impedance with the impedance set point, the microcontroller tuned one syringe pump to infuse or withdraw liquid, hence maintaining a stable liquid film.…”

mentioning

confidence: 99%

See 4 more Smart Citations

Active Tracking of Temporally Changing Gas-Phase Odor Mixture Using an Array of Cells Expressing Olfactory Receptors

et al. 2023

Self Cite

View full text Add to dashboard Cite

A cell expressing an olfactory receptor (OR) exhibits excellent odorant detection ability and thus is widely applied in odor biosensors. Most of those biosensors, however, could detect only liquid-phase nonchanging single-component odorants. In this paper, we raised up an odor biosensor for the active tracking of temporally changing gas-phase odor mixture by an array of cells expressing ORs. A thin stable liquid film covered the cell, thus allowing gas-phase odorants to penetrate. The online image processing generated individual cell brightness data which were used to compute the biosensor response. Based on the obtained responses, we adjusted the known odor components to be similar with the unknown odor. The function of our biosensor was validated by tracking the variable single-component odorant or the binary odor mixture. The influence from the sensor drift could be overcome by comparing the adjacent unknown and known odor responses. In the odor mixture quantification, adding the OR label to mixed cells and then quantifying separately (named as the pre-label method) was more efficient, while directly using the cell response pattern (named as the label-free method) was still capable even if the OR odor had cross-sensitivity.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: ■ Conclusionmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

See 3 more Smart Citations

Active Tracking of Temporally Changing Gas-Phase Odor Mixture Using an Array of Cells Expressing Olfactory Receptors

et al. 2023

Self Cite

View full text Add to dashboard Cite

show abstract

Effective Dissolution of a Gaseous Odorant in Liquid Using Gas Flow

Nakane,

Osaki,

Mimura

et al. 2024

Adv Materials Technologies

View full text Add to dashboard Cite

Olfactory receptors are known for their high specificity, sensitivity, and nanometer‐sized sensing element, making them ideal for in situ odorant sensor applications. However, one of the major limitations of these sensors is the requirement for an aqueous environment to maintain their function, while most odorants are poorly soluble in water. To address this issue, a microfluidic device consisting of a gas‐flow channel and a microwell is proposed that enables the effective dissolution of a gaseous odorant in an aqueous solution. Computational simulations are used to design the gas‐flow channel and the well, and the performance of the odorant dissolution is verified using the fabricated device. This study considers that convection in water induced by the gas flow enhances the dissolution of a gaseous odorant. The feasibility of the device is demonstrated for on‐site detection of a gaseous odorant. This approach facilitates the development of highly responsive and portable sensors utilizing olfactory receptors, offering a promising solution to the limitations of current odorant sensors.

show abstract

Odor Biosensors Based on Cell Expressing Olfactory Receptor: Recent Advances

Deng,

Nakamoto

2024

Analysis & Sensing

View full text Add to dashboard Cite

Inspired by the powerful biological olfaction, scientists extracted numerous materials such as olfactory sensory neuron, olfactory receptor (OR) protein, and odorant binding protein from animal olfactory systems, then combined them with transducers to form multiple odor biosensors. These biosensors, despite well inheriting the sensing ability of creatures, have several drawbacks, such as complex preparation process, unstable sensing material characteristics, and high cost. Unlike the biological materials listed above, cell expressing heterologous OR maintains a stable sensing performance after passaging for multiple generations, also its experimental operation is simple, and cost is low. Therefore, odor biosensors based on cell expressing OR have been well developed in recent years. In this review, we first listed several odor biosensors based on cell expressing OR, mainly focusing on fluorescent and electrophysiological measurement methods. Furthermore, we illustrated the techniques to improve the biosensor performance, e.g., wider detection range, longer lifetime, more OR types, and higher quantification efficiency. In addition, we explained the possible prospects such as big sensor array and predicting odor response.

show abstract

Extending lifetime of gas-phase odor biosensor using liquid thickness control and liquid exchange

Cited by 10 publications

References 28 publications

Active Tracking of Temporally Changing Gas-Phase Odor Mixture Using an Array of Cells Expressing Olfactory Receptors

Active Tracking of Temporally Changing Gas-Phase Odor Mixture Using an Array of Cells Expressing Olfactory Receptors

Effective Dissolution of a Gaseous Odorant in Liquid Using Gas Flow

Odor Biosensors Based on Cell Expressing Olfactory Receptor: Recent Advances

Contact Info

Product

Resources

About