Active Tracking of Temporally Changing Gas-Phase Odor Mixture Using an Array of Cells Expressing Olfactory Receptors

Deng, Hongchao; Sukekawa, Yuji; Mitsuno, Hidefumi; Kanzaki, Ryohei; Nakamoto, Takamichi

doi:10.1021/acs.analchem.3c02675

Cited by 5 publications

(4 citation statements)

References 27 publications

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“…In this study, we showed discrete and continuous odor quantification towards gas-phase odorants. We fixed the cell radius to decrease the noise in image processing, thereby achieving higher quantification resolution than our previous results [21,26]. Single component odor and binary odor mixture were quantified and different target odor stimulation durations were tested to prove the feasibility of these methods.…”

Section: Discussionmentioning

confidence: 99%

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Discrete and continuous odor quantification in gas-phase odor biosensor

Deng,

Mitsuno,

Kanzaki

et al. 2024

Meas. Sci. Technol.

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Cells expressing heterologous olfactory receptors were commonly used as the sensing elements in odor biosensors owing to their high sensitivity and selectivity. However, The odor quantification performance of these biosensors especially in speed and accuracy aspects is not so favorable yet. To overcome this problem, we proposed discrete and continuous odor quantification. In this study, two olfactory receptors, Or13a and Or56a, and their agonists, 1-octen-3-ol and geosmin were used. The cell images were handled online to accelerate the processing speed. The odor intensity that we intended to quantify was the target odor, the odor intensity that we provided was the generated odor. The target and generated odors were repeatedly applied thus mitigating the sensor drift and aging influence. The target odor quantification outcome could be one of several discrete candidates (discrete quantification) or a continuous value computed based on the standard response curve (continuous quantification). We first obtained the typical results of the discrete and continuous quantification methods using single OR. After this, we executed the experiments in multiple target odor intensities and made a comparison between two methods. Discrete quantification was simpler while continuous quantification was faster and more accurate. In addition, both methods were tested under binary odor mixture conditions and their quantification results were satisfactory. The methods proposed here will benefit the on-site precise quantification of odor biosensors.

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Section: Discussionmentioning

confidence: 99%

“…This thin liquid film enabled shorter response time for our biosensor [25]. Then we utilized this gas-phase odor biosensor for actively tracking temporally changing odorant [26]. Although so many efforts have been made, the quantification speed was not fast enough and the quantification accuracy was relatively low.…”

Section: Introductionmentioning

confidence: 99%

Discrete and continuous odor quantification in gas-phase odor biosensor

Deng,

Mitsuno,

Kanzaki

et al. 2024

Meas. Sci. Technol.

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“…The cells expressing OR has a similar function to OSN. Meanwhile, it can be passaged for many generations with steady characteristics, and the OR types can be freely determined by the researchers [51,52,89,90]. Table 1 lists several examples of cells expressing OR-based odor biosensors.…”

Section: Cell's Expressing Olfactory-receptor-based Odor Biosensormentioning

confidence: 99%

“…(m) Extend lifetime of gas-phase odor biosensor using liquid thickness control and liquid exchange [51]. (n) Actively track temporally changing gas-phase odor mixture [52].…”

Section: Introductionmentioning

confidence: 99%

Biosensors for Odor Detection: A Review

Deng,

Nakamoto

2023

Biosensors

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Animals can easily detect hundreds of thousands of odors in the environment with high sensitivity and selectivity. With the progress of biological olfactory research, scientists have extracted multiple biomaterials and integrated them with different transducers thus generating numerous biosensors. Those biosensors inherit the sensing ability of living organisms and present excellent detection performance. In this paper, we mainly introduce odor biosensors based on substances from animal olfactory systems. Several instances of organ/tissue-based, cell-based, and protein-based biosensors are described and compared. Furthermore, we list some other biological materials such as peptide, nanovesicle, enzyme, and aptamer that are also utilized in odor biosensors. In addition, we illustrate the further developments of odor biosensors.

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Odor Biosensors Based on Cell Expressing Olfactory Receptor: Recent Advances

Deng,

Nakamoto

2024

Analysis & Sensing

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Inspired by the powerful biological olfaction, scientists extracted numerous materials such as olfactory sensory neuron, olfactory receptor (OR) protein, and odorant binding protein from animal olfactory systems, then combined them with transducers to form multiple odor biosensors. These biosensors, despite well inheriting the sensing ability of creatures, have several drawbacks, such as complex preparation process, unstable sensing material characteristics, and high cost. Unlike the biological materials listed above, cell expressing heterologous OR maintains a stable sensing performance after passaging for multiple generations, also its experimental operation is simple, and cost is low. Therefore, odor biosensors based on cell expressing OR have been well developed in recent years. In this review, we first listed several odor biosensors based on cell expressing OR, mainly focusing on fluorescent and electrophysiological measurement methods. Furthermore, we illustrated the techniques to improve the biosensor performance, e.g., wider detection range, longer lifetime, more OR types, and higher quantification efficiency. In addition, we explained the possible prospects such as big sensor array and predicting odor response.

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Active Tracking of Temporally Changing Gas-Phase Odor Mixture Using an Array of Cells Expressing Olfactory Receptors

Cited by 5 publications

References 27 publications

Discrete and continuous odor quantification in gas-phase odor biosensor

Discrete and continuous odor quantification in gas-phase odor biosensor

Biosensors for Odor Detection: A Review

Odor Biosensors Based on Cell Expressing Olfactory Receptor: Recent Advances

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