Extenders Containing Dimethylformamide Associated or Not with Glycerol are Ineffective for Ovine Sperm Cryopreservation

Abstract

The study aimed at testing the effectiveness of dimethylformamide, alone or combined with glycerol, as cryoprotectant for freezing ram semen. Ejaculates from nine rams were cryopreserved in Tris-based extenders, containing 5% of glycerol, association of dimethylformamide with glycerol, in four proportions achieving 5% of cryoprotectors in the media and pure dimethylformamide (2, 3, 4 and 5%) in replacement to glycerol. The samples were diluted to 100 × 10(6) sptz/ml and stored in 0.25-ml straws in liquid nitrogen. After thawing (37 °C for 30 s), motility was preserved better by the extender containing 5% of glycerol (p < 0.05). The extenders containing pure dimethylformamide, or more than 2% in combination with glycerol, provided sperm motilities close to zero. Plasma and acrosomal membrane integrity were preserved better (p < 0.05) in the extender containing 5% glycerol. It can be concluded that dimethylformamide, alone or combined with glycerol, has no beneficial effects on ovine semen cryopreservation.

Kualitas Semen Segar Sapi Bali Umur Produktif dan Non-produktif serta Penentuan Konsentrasi Krioprotektan dalam Pengencer Tris Kuning Telur

“…Krioprotektan yang umum digunakan adalah gliserol. Berbagai jenis amida telah digunakan sebagai krioprotektan pada tahun 2000an, salah satunya dimethylformamide (DMF) pada ternak kuda (Osorio et al, 2008;Hoffman et al, 2011), pada ternak kambing (Moustacas et al, 2011;Ariantie et al, 2013). Krioprotektan DMF mempunyai bobot molekul yang lebih kecil daripada gliserol, sehingga daya penetrasinya lebih baik.…”

Treating boar sperm with cholesterol-loaded cyclodextrins or cyclodextrins prior to cryopreservation: effects on post-thaw in vitro sperm quality of sperm cryopreserved in different freezing extenders.

“…Sperm viability was similar among the other treatments, indicating that MA, DMA, MF and DMF do not produce immediate detrimental effects on sperm. Our findings were similar to the results found in dog and ram (Moustacas et al, 2011) where MF and DMF were tested, but differ from previous studies in boar where A at concentrations ≤ 5.9% (w:v) did not affect the percentage of motile sperm . It is likely that this disagreement is due to the differences in the protocol followed to treat and evaluate the sperm after diluting with the freezing extenders (dilution of the samples or not in cryoprotectant-free diluents before evaluating the sperm quality).…”

Dimetilacetamida Associada Ou Não Ao Glicerol Para Criopreservação De Sêmen Ovino

“…Outros crioprotetores da função química amida foram avaliados para conservação de espermatozoides ovinos, dentre eles a dimetilformamida (DMF), metilformamida (MF) e acetamida. A eficácia da DMF foi estudada por Moustacas et al (29) e foi observada grande variação (P<0,05) na motilidade progressiva entre os tratamentos com DMF, isolada ou em associação com glicerol e o grupo controle (GL5%). Esses autores concluíram que a DMF não apresentou benefícios sobre os espermatozoides submetidos a congelamento, mesmo na presença de glicerol na composição dos diluidores.…”

“…Esses autores concluíram que a DMF não apresentou benefícios sobre os espermatozoides submetidos a congelamento, mesmo na presença de glicerol na composição dos diluidores. Apesar de amidas distintas (DMA versus DMF), os achados do presente trabalho discordam dos relatados por Moustacas et al (29) , já que foram obtidos resultados satisfatórios para os grupos com amida associada ao glicerol, respeitando o limite máximo de 3% de DMA.…”