Extended storage of platelets in SSP⁺ platelet additive solution

Abstract

The results suggest that SSP+ could be used for platelet storage for up to 9 days. However, the preparation of platelets in the additive requires some optimization. In vivo studies are required to confirm these in vitro results.

Quality Assessment of Established and Emerging Blood Components for Transfusion

“…As is the case with HSR, the ESC response of platelets declines during storage [117, 120, 121]. Lactate dehydrogenase (LDH) release into the supernatant can be measured as an indicator of platelet lysis and loss of viability [122, 123], often accompanied by a reduction in platelet count and swelling, measured by an increase in mean platelet volume (MPV).…”

“…Therefore, measurement of glucose consumption, lactate production, and changes in pH during platelet storage are widely used as indicators of active metabolism, as are measurements of pO 2 , pCO 2 , and HCO 3 [123, 125–128]. Platelet surface markers are also indicative of platelet activation, including CD62P (P-selectin) and exposure of phosphatidylserine (PS) measured by annexin V binding, and are typically measured by flow cytometry [129, 130].…”

“…Platelet surface markers are also indicative of platelet activation, including CD62P (P-selectin) and exposure of phosphatidylserine (PS) measured by annexin V binding, and are typically measured by flow cytometry [129, 130]. As platelets become activated, the contents of their granules, such as RANTES, CD40L, soluble CD62P, and platelet factor 4 (PF4), are released and accumulate in PCs during storage [122, 123, 129–131]. …”

“…CD62P (also known as P-selectin) is a well-characterized marker of platelet activation, which increases when platelets are stored at room temperature [122, 123, 173]. Given that cryopreserved platelets are known to be activated, it would be expected that CD62P expression would be high on cryopreserved platelets.…”

Impact of sample volume and handling time during analysis on the in vitro quality measurements of platelet concentrates held in syringes

“…Although the metabolic markers glucose and lactate did not change during the time course, the partial pressure of oxygen (pO 2 ) and carbon dioxide (pCO 2 ) had the most significant changes. A decrease in pO 2 and an increase in pCO 2 are indicative of an increased metabolic rate (Hornsey et al. , 2006) most likely triggered by the sample hold time; however, in this study, it was a reflection of a handling artifact and not the conditions in the platelet storage container.…”