Extended-spectrum β-lactamase-producing Escherichia coli from extraintestinal infections in humans and from food-producing animals in Italy: a ‘One Health’ study

Giufrè, Maria; Mazzolini, Elena; Cerquetti, Marina; Brusaferro, Silvio; Accogli, Marisa; Agnoletti, Fabrizio; Agodi, Antonella; Alborali, Giovanni Loris; Arghittu, Milena; Auxilia, F.; Barchitta, Martina; Bosco, Natasha; Camporese, Alessandro; Carfora, Virginia; Collini, Lucia; D’Agaro, Pierlanfranco; Rosa, Rita De; Formenti, Nicoletta; Franco, Alessia; Koncan, Raffaella; Lanzafame, Paolo; Mazzariol, Annarita; Moschioni, Chiara; Pane, Stefania; Putignani, Lorenza; Thoma, Claudia

doi:10.1016/j.ijantimicag.2021.106433

Cited by 28 publications

(22 citation statements)

References 34 publications

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“…Molecular characterization of the ESBL isolates revealed the predominance of CTX-M type genes, mainly those of group 1, which is in accordance with preexisting literature about pigs [ 52 , 53 , 54 ]. Interestingly, various other genes conferring resistance to fluoroquinolones, aminoglycosides, sulfonamides, trimethoprim, macrolides and colistin were detected in the present study.…”

Section: Discussionsupporting

confidence: 88%

“…Recent studies in Greece and worldwide have described the AMR profile of ESBL-producing E. coli strains isolated from livestock, including pigs [ 9 , 45 , 54 ]. Currently, as a wide dissemination of multidrug resistant bacteria in diverse ecosystems is observed, the establishment of an integrated antimicrobial surveillance system under the One Health approach is crucial for the protection of public health.…”

Section: Discussionmentioning

confidence: 99%

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Cross-Sectional Survey of Antibiotic Resistance in Extended Spectrum β-Lactamase-Producing Enterobacteriaceae Isolated from Pigs in Greece

Tsekouras

Diezel

Kostoulas

et al. 2022

Animals

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This study aimed to estimate the prevalence of extended-spectrum β-lactamase-producing (ESBL) bacteria in swine. Thus, 214 fecal samples were collected from suckling and weaned piglets from 34 farms in Greece (out of an overall population of about 14,300 sows). A subset of 78 (36.5%) ESBL producers were identified as E. coli (69/78, 88.5%), K. pneumoniae spp. pneumoniae (3.8%), P. mirabilis (5.1%), E. cloacae complex (1.3%) and S. enterica spp. diarizonae (1.3%). Resistance to at least one class of non-β-lactam antibiotics was detected in 78 isolates. Among the E. coli strains, resistance was identified with regard to aminoglycosides (n = 31), fluoroquinolones (n = 49), tetracycline (n = 26) and trimethoprim/sulfamethoxazole (n = 46). Of the three K. pneumoniae spp. pneumoniae, two displayed resistances to aminoglycosides and all were resistant to fluoroquinolones, tetracyclines and trimethoprim/sulfamethoxazole. As for the four P. mirabilis isolates, three had a resistant phenotype for aminoglycosides and all were resistant to imipenem, fluoroquinolones, tetracyclines and trimethoprim/sulfamethoxazole. Molecular characterization of the isolates revealed the presence of CTX-M, SHV and TEM genes, as well as of genes conferring resistance to fluoroquinolones, aminoglycosides, sulfonamides, trimethoprim, macrolides and colistin. High levels of antimicrobial resistance (AMR) were demonstrated in Greek swine herds posing a concern for the efficacy of treatments at the farm level as well as for public health.

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Section: Discussionsupporting

confidence: 88%

Section: Discussionmentioning

confidence: 99%

Cross-Sectional Survey of Antibiotic Resistance in Extended Spectrum β-Lactamase-Producing Enterobacteriaceae Isolated from Pigs in Greece

Tsekouras

Diezel

Kostoulas

et al. 2022

Animals

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“…In Egypt, ESBL-producing E. coli was previously reported in chickens, chicken meat [17,18], and humans [3], which highlights the importance of continuous monitoring ESBLproducing E. coli in both animals and humans. Hence, this study was aimed to investigate the phenotypic and genotypic characteristics of ESBL-producing E. coli samples obtained from diseased chickens and compared with samples retrieved from human cases in Egypt in the period between 2019 and 2020.…”

Section: Introductionmentioning

confidence: 97%

“…The potential zoonotic importance of E. coli either from animals or food derived from animals has been previously documented. In the last two decades, E. coli-producing ESBLs have been reported widely in animals as well as humans possessing a serious public-health threat [3,4].…”

Section: Introductionmentioning

confidence: 99%

Multidrug-Resistant and Genetic Characterization of Extended-Spectrum Beta-Lactamase-Producing E. coli Recovered from Chickens and Humans in Egypt

Badr¹,

Reda²,

Hagag³

et al. 2022

Animals

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Colonization of food chain animals such as chickens with extended-spectrum β-lactamases (ESBL) poses a major health threat to human. The current study aimed to determine the phenotypic and genotypic relationship between ESBL-producing E. coli from diseased human and chickens in Egypt. A total of 56 out of 120 chicken farms (46.7%) and 9 human samples (100%) were phenotypically and genotypically identified with at least one ESBL-phenotype/gene. Chicken isolates showed a high proportion of beta lactamase from CTX-M group 9 > TEM > PER families, followed by CTX-M group 1 > SHV > GES > OXA group10 > VEB > OXA group2 families, while human isolates only contained the CTX-M family. A high incidence of ESBL genes from the CTX-M family was recognized in both human and chicken isolates. Furthermore, nucleotide identity showed high similarity between chicken and human isolates. In conclusion, the current study traced phenotypes and genotypes of ESBL-producing E. coli from chickens and human samples in Egypt, reporting degrees of similarity that suggest potential zoonotic transmission. Our data highlighted the significant importance of chicken as a major food source not only in Egypt but all over the world in the spreading of ESBL-producing E. coli to human.

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“…Microorganisms are considered to be concerned with several life-threatening infections both in humans and animals [ 29 ]. The E. coli and S. aureus are often found in food, environment, and humans as well as animal intestines [ 30 ], and can cause serious skin infections, respiratory and excretory tract issues [ 31 ]. Different antibiotics were used to treat these harmful bacteria, however, their continued use or intake led to the formation of drug-resistant bacteria [ 32 ].…”

Section: Introductionmentioning

confidence: 99%

Optimization of Platinum Nanoparticles (PtNPs) Synthesis by Acid Phosphatase Mediated Eco-Benign Combined with Photocatalytic and Bioactivity Assessments

et al. 2022

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Noble metal nanoparticles (NMNPs) are viable alternative green sources compared to the chemical available methods in several approach like Food, medical, biotechnology, and textile industries. The biological synthesis of platinum nanoparticles (PtNPs), as a strong photocatalytic agent, has proved as more effective and safer method. In this study, PtNPs were synthesized at four different temperatures (25 °C, 50 °C, 70 °C, and 100 °C). PtNPs synthesized at 100 °C were smaller and exhibited spherical morphology with a high degree of dispersion. A series of physicochemical characterizations were applied to investigate the synthesis, particle size, crystalline nature, and surface morphology of PtNPs. The biosynthesized PtNPs were tested for the photodegradation of methylene blue (MB) under visible light irradiations. The results showed that PtNPs exhibited remarkable photocatalytic activity by degrading 98% of MB only in 40 min. The acid phosphatase mediated PtNPs showed strong bacterial inhibition efficiency against S. aureus and E. coli. Furthermore, it showed high antioxidant activity (88%) against 1,1-diphenyl-2-picryl-hydrazil (DPPH). In conclusion, this study provided an overview of the applications of PtNPs in food chemistry, biotechnology, and textile industries for the deterioration of the natural and synthetic dyes and its potential application in the suppression of pathogenic microbes of the biological systems. Thus, it could be used as a novel approach in the food microbiology, biomedical and environmental applications.

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Extended-spectrum β-lactamase-producing Escherichia coli from extraintestinal infections in humans and from food-producing animals in Italy: a ‘One Health’ study

Cited by 28 publications

References 34 publications

Cross-Sectional Survey of Antibiotic Resistance in Extended Spectrum β-Lactamase-Producing Enterobacteriaceae Isolated from Pigs in Greece

Cross-Sectional Survey of Antibiotic Resistance in Extended Spectrum β-Lactamase-Producing Enterobacteriaceae Isolated from Pigs in Greece

Multidrug-Resistant and Genetic Characterization of Extended-Spectrum Beta-Lactamase-Producing E. coli Recovered from Chickens and Humans in Egypt

Optimization of Platinum Nanoparticles (PtNPs) Synthesis by Acid Phosphatase Mediated Eco-Benign Combined with Photocatalytic and Bioactivity Assessments

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