Extended-spectrum beta-lactamases in ceftazidime-resistant &lt;i&gt;Escherichia coli &lt;/i&gt;and &lt;i&gt;Klebsiella pneumoniae &lt;/i&gt;isolates in Turkish hospitals

Hoşoğlu, Salih; Gündeş, Sibel; Kolayli, Fetiye; Karadenizli, Aynur; Demirdag, Kutbettin; Günaydın, Murat; Altındiş, Mustafa; Çaylan, Refik; Uçmak, Hasan

doi:10.4103/0255-0857.37336

Cited by 41 publications

(26 citation statements)

References 20 publications

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“…Other ESBL genes such as TEM, OXA, and CTX-M may be responsible for ESBL phenotype (21). The rate of blaSHV in this study was very similar to that reported in the Swedish hospital (6%-2001 to 2006) (22), was nearly similar to that in Seoul National University Hospital in Korea (8.7% -1995 to 1999) (23), and a little higher than that reported in Thailand (3.8% -2005) (24); it was lower than that reported in university hospital in Salamanca, Spain (15.6%-2001 to 2004) (25) and in Turkish hospitals( 28.6%-2007) (26).…”

Section: Discussionsupporting

confidence: 50%

Prevalence of AmpC and SHV β-Lactamases in Clinical Isolates of Escherichia coli From Tehran Hospitals

Dallal

Sabbaghi

Aghamirzaeie

et al. 2013

Jundishapur J Microbiol

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Background: Beta-lactam resistance in Gram-negative bacteria, especially Escherichia coli, is a main clinical problem. It is often caused by the coproduction of β-lactamases, particularly extended-spectrum β-lactamases (ESBLs) and AmpC enzymes. It may lead to a problem for diagnosis via recommended phenotypic tests by the Clinical and Laboratory Standards Institute (CLSI). Conversely, β-lactamase genes have several subfamilies; therefore, using designed primers with high ability could be valuable to detect all of them. Objectives: This investigation focused on evaluating the prevalence of extended-spectrum β-lactamases using disk diffusion method and confirmatory test (Combined Disk), as well as PCR for complete detection of these genes (SHV-and AmpC (CITM, FOX)-type β-lactamase genes). Materials and Methods: 500 clinical samples were collected from different hospitals of Tehran, Iran and 200 E. coli isolates were detected by standard biochemical tests such as IMVIC. These specimens were isolated from urine, stool, blood, and wound. Subsequently, the isolates were screened by disk diffusion method and combined disk assay for β-lactamase production. Resistant isolates were evaluated by PCR for molecular assessing. Results: In disk agar diffusion test, 128(64%) E. coli isolates resistant to ceftazidime (55.5%) and cefotaxime (64%) were selected and followed by combined disk (ceftazidime, cefotaxime, and clavulanic acid) assay. In combined disk assay, among 128 screened isolates, 115 (89.8%) and 13 (10.2%) isolates were detected as ESBLs and AmpC producers, respectively. PCR performed on all 128 selected isolates via phenotypic tests and the results showed that among 115 and 13 isolates, 7 (6.1%) and 13(100%) cases contained blaSHV and blaCITM, respectively. Fox gene was not detected in any sample. Conclusions: Our findings indicate that the prevalence of ESBLs in Tehran is rising. According to CLSI, isolates showing negative confirmatory test are potentially considered as producers of AmpC (the result of CITM PCR was 100% positive). On the other hand, co-production of ESBLs and AmpC may lead to ESBLs false negative. Thus, development of diagnosis methods for complete detection of β-lactamase enzymes is important for resistance control and the treatment with high achievement.

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Section: Discussionsupporting

confidence: 50%

Prevalence of AmpC and SHV β-Lactamases in Clinical Isolates of Escherichia coli From Tehran Hospitals

Dallal

Sabbaghi

Aghamirzaeie

et al. 2013

Jundishapur J Microbiol

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“…In many studies, a remarkable increase in the ESBL rate was reported from all regions of the world (Eisner et al, 2006;Gupta, 2007;Hosoglu et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Mutations in -lactamases detected in multidrug resistant gram negative bacteria isolated from community acquired urinary tract infections in Assiut, Egypt

Salah¹,

Azab²,

Halaby³

et al. 2016

Afr. J. Microbiol. Res.

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“…Extended spectrum β-lactamases are a large, rapidly evolving group of plasmid mediated enzymes capable of hydrolyzing and inactivating penicillins, cephalosporins and monobactams and are inhibited by β-lactamase inhibitors such as clavulanate, sulbactam and tazobactam 6,7,8 . Since their description in the mid-1980s, ESBLs spread rapidly to Europe, US and Asia and are now found all over the world 9 .…”

Section: Introductionmentioning

confidence: 99%

Frequency and Antimicrobial Sensitivity Pattern of Extended Spectrum β-Lactamases Producing Escherichia coli and Klebsiella pneumoniae Isolated from urine at a Tertiary Care Hospital

Islam

Jhora²,

Yusuf

et al. 2012

J Shaheed Suhrawardy Med Coll

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Background: Infections due to extended spectrum β-lactamases (ESBL) producing Escherichia coli and Klebsiella pneumoniae have become an important clinical problem. These organisms are important regarding the infection control by the physicians. Objective: The present study was undertaken to determine the prevalence of ESBLs along with their antimicrobial sensitivity pattern in Escherichia coli and Klebsiella pneumoniae. Methodology: This cross sectional study was conducted in the Department of Microbiology at Sir Salimullah Medical College, Dhaka. Urine samples were collected from patients who were clinically suspected to have UTI. After incubation, plates were checked for presence of suspected pathogens. Organisms were identified to species level by conventional methods. All isolated E. coli and K. pneumoniae were included in the study. The susceptibility to antibiotics was determined by Kirby Bauer method on Muller Hinton agar. Isolates were screened for ESBL production by using disk diffusion of cefotaxime, ceftazidime, ceftriaxone and cefpodoxime placed on inoculated plates containing Muller Hinton agar according to the CLSI recommendations. Phenotypic confirmatory test for ESBL producers was done by combined disc diffusion for all the isolates that were screened positive for the ESBL production following CLSI guidelines. Combined disk diffusion method was also done in this study. Result: A total of 220 non repeated urine samples were cultured of which 132(60%) cases had shown the bacterial growth. Among the 132 samples Escherichia coli had found in 103(78.0%) cases and Klebsiella spp. was found in 14(10.6%) cases. Out of 103 E coli 23(22.3%) cases was found as ESBL strain. On the other hand within 14 Klebsiella species, the ESBL strain was found in 5(35.7%) cases. Both E coli and Klebsiella species were 100% sensitive to imipenem. However, cephamycin was sensitive in 93.7% and 100% in E coli and Klebsiella species respectively. Conclusion: Results indicate that routine ESBL detection should be made imperative and empirical use of third generation cephalosporins must be discouraged.

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Extended-spectrum beta-lactamases in ceftazidime-resistant <i>Escherichia coli </i>and <i>Klebsiella pneumoniae </i>isolates in Turkish hospitals

Cited by 41 publications

References 20 publications

Prevalence of AmpC and SHV β-Lactamases in Clinical Isolates of Escherichia coli From Tehran Hospitals

Prevalence of AmpC and SHV β-Lactamases in Clinical Isolates of Escherichia coli From Tehran Hospitals

Mutations in -lactamases detected in multidrug resistant gram negative bacteria isolated from community acquired urinary tract infections in Assiut, Egypt

Frequency and Antimicrobial Sensitivity Pattern of Extended Spectrum β-Lactamases Producing Escherichia coli and Klebsiella pneumoniae Isolated from urine at a Tertiary Care Hospital

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Extended-spectrum beta-lactamases in ceftazidime-resistant <i>Escherichia coli </i>and <i>Klebsiella pneumoniae </i>isolates in Turkish hospitals

Cited by 41 publications

References 20 publications

Prevalence of AmpC and SHV &#946;-Lactamases in Clinical Isolates of Escherichia coli From Tehran Hospitals

Prevalence of AmpC and SHV &#946;-Lactamases in Clinical Isolates of Escherichia coli From Tehran Hospitals

Mutations in -lactamases detected in multidrug resistant gram negative bacteria isolated from community acquired urinary tract infections in Assiut, Egypt

Frequency and Antimicrobial Sensitivity Pattern of Extended Spectrum &beta;-Lactamases Producing Escherichia coli and Klebsiella pneumoniae Isolated from urine at a Tertiary Care Hospital

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Prevalence of AmpC and SHV β-Lactamases in Clinical Isolates of Escherichia coli From Tehran Hospitals

Prevalence of AmpC and SHV β-Lactamases in Clinical Isolates of Escherichia coli From Tehran Hospitals

Frequency and Antimicrobial Sensitivity Pattern of Extended Spectrum β-Lactamases Producing Escherichia coli and Klebsiella pneumoniae Isolated from urine at a Tertiary Care Hospital