Extended Spectrum Beta-Lactamase-Resistant Determinants among Carbapenem-Resistant Enterobacteriaceae from Beef Cattle in the North West Province, South Africa: A Critical Assessment of Their Possible Public Health Implications

Tshitshi, Lungisile; Manganyi, Madira Coutlyne; Montso, Peter Kotsoana; Mbewe, Moses; Ateba, Collins Njie

doi:10.3390/antibiotics9110820

Cited by 20 publications

(20 citation statements)

References 50 publications

(54 reference statements)

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“…The beta-lactamase enzyme (bla TEM ) was firstly identified in K. pneumoniae and Gram-negative bacteria (Tshitshi et al, 2020). Mutation of TEM genes could occur if a single amino acid or multiplier were substituted around the active site of its enzyme (Dantas and Ferreira, 2020) The mobile genetic elements (MGE) could transfer the resistant genes in beta-lactamase-producing enzymes bacteria (Eka and Pinatih, 2017).…”

Section: Advances In Animal and Veterinary Sciencesmentioning

confidence: 99%

Molecular Studies on Some Virulent and Multi-Drug Resistant Cattle Klebsiella Strains and their Heamatobiochemical Impacts

Ibrahim¹,

Helal²,

Kamoura³

et al. 2023

AAVS

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Multidrug resistant Klebsiella spp. (MDR) and the extended-spectrum 𝛽-lactamases (ESBL) producing strains are one of the dominantly pathogenic bacteria that involved in bovine respiratory infections. Its spreading problem is growing rapidly especially it could combine resistance with high-level of virulence traits that make it is more difficult to be treated. This study provides insight into the respiratory infections by Klebsiella spp. and its MDR profile in bovine cases, as well as the potential target genes that were involved in the links between virulence determinants and MDR. The bacterial examination of 200 nasal swabs and 30 lung tissue samples (taken from 30 emergency slaughtered cattle) indicated that Klebsiella spp. was recovered in 55/230 (24%) of all samples with a high proportion of K. pneumoniae (18.7%). In-vitro antibiogram exhibited a wide MDR phenotype. The virulence genes (fimA, aerobactin, rmpA and magA) were exhibited in 90%, 85%, 90% and 80% of the tested isolates, respectively. Moreover, ESBL-resistant markers (bla CTX-M , bla TEM and bla SHV ) were found in 100%, 85% and 80% of the same isolates, respectively. A significant positive correlation (R = 0.7) was reported between aerobactine and magA virulence genes and bla TEM and bla SHV ESBL resistant genes, respectively. In addition, the hemato-biochemical analysis of 100 blood samples (50 from naturally infected and 50 from apparently healthy cattle) revealed significant decreases in the haemobiogram parameters levels meanwhile, leucogram picture total proteins, globulins, urea, creatinine levels were significantly increased in klebsiella-infected animals. An alarming increase in MDR and ESBL klebsiella bovine infections necessitates a controlled use of antibiotics in cattle farms and warrants sustainable monitoring of antibiotic emergence events and further studies for their genetic phenotypic interrelation. Moreover, the haemato-biochemical alterations in the klebsiella infected cattle could be act as biomarkers for bacterial infections.

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Section: Advances In Animal and Veterinary Sciencesmentioning

confidence: 99%

Molecular Studies on Some Virulent and Multi-Drug Resistant Cattle Klebsiella Strains and their Heamatobiochemical Impacts

Ibrahim¹,

Helal²,

Kamoura³

et al. 2023

AAVS

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“…To date, antibiotic therapy is still regarded as an essential treatment of secondary infections [ 39 ], despite the rise in microbial resistance, hence the evolution of chronic diseases [ 40 , 41 ]. A number of studies have reported antimicrobial properties such as antibacterial, antifungal and antiviral properties of the genera Drimia in both in vitro and in vivo models.…”

Section: Biological Activities Of Drimia Sppmentioning

confidence: 99%

Bulbous Plants Drimia: “A Thin Line between Poisonous and Healing Compounds” with Biological Activities

et al. 2021

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Drimia (synonym Urginea) plants are bulbous plants belonging to the family Asparagaceae (formerly the family Hyacinthaceae) and are distinctive, powerful medicinal plants. Just some species are indigenous to South Africa and have been traditionally utilized for centuries to cure various diseases and/or ailments. They have been recognized among the most famous and used medicinal plants in South Africa. Traditionally, the plants are used for various illnesses such as dropsy, respiratory disease, bone and joint complications, skin disorders, epilepsy and cancer. A number of studies have reported biological properties such as antiviral, antibacterial, antioxidant and anti-inflammatory, immunomodulatory, and anticancer activities. Their bulbs are a popular treatment for colds, measles, pneumonia, coughs, fever and headaches. However, some plant species are regarded as one of the six most common poisonous plants in Southern Africa that are toxic to livestock and humans. Due to the therapeutic effects of the Drimia plant bulb, research has focused on the phytochemicals of Drimia species. The principal constituents isolated from this genus are cardiac glycosides. In addition, phenolic compounds, phytosterols and other phytochemical constituents were identified. This study constitutes a critical review of Drimia species’ bioactive compounds, toxicology, biological properties and phytochemistry, advocating it as an important source for effective therapeutic medicine. For this purpose, various scientific electronic databases such as ScienceDirect, Scopus, Google Scholar, PubMed and Web of Science were researched and reviewed to conduct this study. Despite well-studied biological investigations, there is limited research on the toxic properties and the toxic compounds of certain Drimia species. Searching from 2017 to 2021, Google Scholar search tools retrieved 462 publications; however, only 3 investigated the toxicity and safety aspects of Drimia. The aim was to identify the current scientific research gap on Drimia species, hence highlighting a thin line between poisonous and healing compounds, dotted across numerous publications, in this review paper.

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“…The antimicrobial resistance profiles of the isolates were determined by the disc diffusion assay on Mueller Hinton agar following the CLSI (Clinical Laboratory Standards Institute) recommendations [50]. Twelve antibiotics (Mast Diagnostics, UK) belonging to seven groups were used: β-lactam/ β-lactamase inhibitor (Piperacillin-tazobactam, 100 μg /10 μg), Carbapenem (Meropenem, 10 μg), Monobactam (Aztreonam, 30 μg), Aminoglycoside (Gentamicin, 10 μg; Amikacin, 30 μg), Quinolone (Ciprofloxacin), Cephem (Cefepime, 30 μg; Cefotaxime, 30 μg; Ceftriaxone, 30 μg; Cefuroxime, 30 μg and Ceftazidime, 30 μg) and folate pathway inhibitor (Trimethoprim-Sulfamethoxazole, 1.25/ 23.75 μg).…”

Section: Antimicrobial Susceptibility Testingmentioning

confidence: 99%

“…Twelve antibiotics (Mast Diagnostics, UK) belonging to seven groups were used: β-lactam/ β-lactamase inhibitor (Piperacillin-tazobactam, 100 μg /10 μg), Carbapenem (Meropenem, 10 μg), Monobactam (Aztreonam, 30 μg), Aminoglycoside (Gentamicin, 10 μg; Amikacin, 30 μg), Quinolone (Ciprofloxacin), Cephem (Cefepime, 30 μg; Cefotaxime, 30 μg; Ceftriaxone, 30 μg; Cefuroxime, 30 μg and Ceftazidime, 30 μg) and folate pathway inhibitor (Trimethoprim-Sulfamethoxazole, 1.25/ 23.75 μg). These antimicrobial agents were selected based on resistance data from some previous studies conducted in the study area [15,50]. In addition, third-generation cephalosporins were included in the evaluation due to the fact that they have a wide activity against gram-negative microorganisms and are very useful for the treatment of serious bacterial infections in humans.…”

Section: Antimicrobial Susceptibility Testingmentioning

confidence: 99%

Evidence of potentially unrelated AmpC beta-lactamase producing Enterobacteriaceae from cattle, cattle products and hospital environments commonly harboring the blaACC resistance determinant

et al. 2021

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The occurrence and genetic relatedness of AmpC beta-lactamase producing Enterobacteriaceae isolated from clinical environments, groundwater, beef, human and cattle faeces were investigated. One hundred seventy-seven (177) samples were collected and cultured on MacConkey agar. A total of 203 non-repetitive isolates were characterised using genus/species-specific PCRs and the identified isolates were subjected to antibiotic susceptibility testing. The production of AmpC beta-lactamases was evaluated using cefoxitin disc, confirmed by the D96C detection test and their encoding genes detected by PCR. The D64C extended-spectrum beta-lactamases (ESBL) test was also performed to appraise ESBLs/AmpC co-production. The genetic fingerprints of AmpC beta-lactamase producers were determined by ERIC-PCR. A total of 116 isolates were identified as E. coli (n = 65), Shigella spp. (n = 36) and Klebsiella pneumoniae (n = 15). Ciprofloxacin resistance (44.4–55.4%) was the most frequent and resistance against the Cephem antibiotics ranged from 15–43.1% for E. coli, 25–36.1% for Shigella spp., and 20–40% for K. pneumoniae. On the other hand, these bacteria strains were most sensitive to Amikacin (0%), Meropenem (2.8%) and Piperacillin-Tazobactam (6.7%) respectively. Nineteen (16.4%) isolates comprising 16 E. coli and 3 Shigella spp. were confirmed as AmpC beta-lactamase producers. However, only E. coli isolates possessed the corresponding resistance determinants: blaACC (73.7%, n = 14), blaCIT (26%, n = 5), blaDHA (11%, n = 2) and blaFOX (16%, n = 3). Thirty-four (27.3%) Enterobacteriaceae strains were confirmed as ESBL producers and a large proportion (79.4%, n = 27) harboured the blaTEM gene, however, only two were ESBLs/AmpC co-producers. Genetic fingerprinting of the AmpC beta-lactamase-producing E. coli isolates revealed low similarity between isolates. In conclusion, the findings indicate the presence of AmpC beta-lactamase-producing Enterobacteriaceae from cattle, beef products and hospital environments that commonly harbour the associated resistance determinants especially the blaACC gene, nonetheless, there is limited possible cross-contamination between these environments.

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Extended Spectrum Beta-Lactamase-Resistant Determinants among Carbapenem-Resistant Enterobacteriaceae from Beef Cattle in the North West Province, South Africa: A Critical Assessment of Their Possible Public Health Implications

Cited by 20 publications

References 50 publications

Molecular Studies on Some Virulent and Multi-Drug Resistant Cattle Klebsiella Strains and their Heamatobiochemical Impacts

Molecular Studies on Some Virulent and Multi-Drug Resistant Cattle Klebsiella Strains and their Heamatobiochemical Impacts

Bulbous Plants Drimia: “A Thin Line between Poisonous and Healing Compounds” with Biological Activities

Evidence of potentially unrelated AmpC beta-lactamase producing Enterobacteriaceae from cattle, cattle products and hospital environments commonly harboring the blaACC resistance determinant

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