Extended-Spectrum Beta-Lactamase Detection with Different Panels for Automated Susceptibility Testing and with a Chromogenic Medium

Färber, Jacqueline; Moder, K.-A.; Layer, Franziska; Tammer, Ina; König, Wolfgang; König, B.

doi:10.1128/jcm.02198-08

Cited by 9 publications

(10 citation statements)

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“…Presently, most of the available diagnostic approaches for the detection of extended‐spectrum beta‐lactamase (ESBL) and carbapenemase producing bacteria are culture‐based, with unacceptable time‐to‐results for effective therapeutic management. (Carrer et al, ; Farber et al, ; Linscott and Brown, ; Reglier‐Poupet et al, ) Such diagnostic approaches include ChromID ESBL, Etest ESBL, and Vitek (bioMerieux). Additionally, there are commercially available solutions for the molecular detection of beta‐lactam resistance in Gram‐negative bacteria, like Check‐MDR (Checkpoints), Evigene (AdvanDx), Hyplex SuperBug ID (Amplex); however, these are all based on complex optical readout systems and are not suitable for POC detection as they also require demanding sample preparation and pre‐analytics.…”

Section: Introductionmentioning

confidence: 99%

Carbon screen‐printed electrodes on ceramic substrates for label‐free molecular detection of antibiotic resistance

Obaje

Cummins

Schulze

et al. 2016

J of Interdiscip Nanomed

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The growing threat posed by antimicrobial resistance on the healthcare and economic well‐being of mankind is pushing the need to develop novel and improved diagnostic platforms for its rapid detection at point of care, facilitating better patient management strategies during antibiotic therapy. In this paper, we present the manufacturing and characterisation of a low‐cost carbon screen‐printed electrochemical sensor on a ceramic substrate. Using label‐free electrochemical impedance spectroscopy, the sensor is demonstrated for the detection of blaNDM, which is one of the main antimicrobial resistance factors in carbapenem‐resistant Enterobacteriaceae. The electrochemical performance of the newly fabricated sensor was initially investigated in relation to the function of its underlying composite materials, evaluating the choice of carbon and dielectric pastes by characterising properties like surface roughness, wetting and susceptibility of unspecific DNA binding. Subsequently, the sensor was used in an electrochemical impedance spectroscopy assay for the sensitive and specific detection of synthetic blaNDM targets achieving a detection limit of 200 nM. The sensor properties and performance demonstrated in this study proved the suitability of the new electrode materials and manufacturing for further point‐of‐care test development as an inexpensive and effective alternative to gold electrodes sensor.

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Section: Introductionmentioning

confidence: 99%

Carbon screen‐printed electrodes on ceramic substrates for label‐free molecular detection of antibiotic resistance

Obaje

Cummins

Schulze

et al. 2016

J of Interdiscip Nanomed

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“…A high rate of detection of ESBL by the E-test could be due to the inclusion of false-positive results. Färber et al [42] reported 6% false-positive results for ESBL E-test in comparison with ESBL-positive strains confirmed by the genotypic method. Notably, the diagnostic accuracy ESBL E-test is only 94% when compared with that of the molecular identification method of ESBL production [43].…”

Section: Discussionmentioning

confidence: 99%

Molecular characterization of extended-spectrum β-lactamase-producing Escherichia coli isolated from postpartum uterine infection in dairy cattle in India

Agrawal

Singh

et al. 2021

Vet World

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Background and Aim: Selection and dissemination of plasmid-encoded extended-spectrum β-lactamase (ESBL) among Enterobacteriaceae confers resistance to beta-lactam antibiotics. The purpose of this study was to determine the prevalence and molecular characteristics of ESBL-producing organisms isolated from dairy cattle with a uterine infection. Materials and Methods: Bacterial isolates (n=62) were characterized by biochemical test for genus and species determination. Antimicrobial susceptibility tests were performed by Kirby–Bauer disk diffusion method using panel of antibiotics for initial screening of ESBL organism. Phenotypic confirmation of ESBL-suspected strains was done by combination disk method and double-disk method. Multiplex polymerase chain reaction (PCR) was carried out for phylogrouping of Escherichia coli isolates as well as for genotyping ESBL genes. Enterobacterial repetitive intergenic consensus-PCR method was used for genotypic characterization of isolates. Results: Antibiotic susceptibility profile of E. coli (n=40) isolates showed high rates of resistance for ampicillin (95.0%), cefpodoxime (97.5%), cefotaxime (87.5%), and ceftriaxone (70%). However, low rates of resistance were observed for cefoxitin (25%), amoxicillin/clavulanic acid (20%), ceftazidime (17.5%), gentamicin (10%), and ertapenem (7.5%). A total of 39/40 E. coli isolates were confirmed as ESBL with Epsilometer test as well as the genotypic method and 28 (70%) of them were multidrug-resistant. Genotype blaCTX-M was observed as a predominant beta-lactamase type with the preponderance of CTX-M Group 1. The following combinations were observed: blaTEM + blaCTX-M in 15 (36.2%) isolates, blaTEM / blaSHV in 8 (5.2%) isolates, and blaCTX-M / blaSHV in 6 (5.2%) isolates. The phylogenetic grouping of E. coli strains revealed the highest prevalence for B1 (22.0%) followed by A (20%). Conclusion: This report shows a high frequency of ESBL E. coli from cattle with postpartum uterine infections. These isolates showed reduced susceptibility to common antibiotics used for the treatment of uterine infections greater affecting the therapeutic outcome.

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“…The BD Phoenix ESBL test utilized the growth response to cefotaxime, ceftazidime, cefpodoxime, and ceftriaxone, with or without clavulanic acid, to detect the production of ESBL. The results were analyzed using the BDXpert system (Nippon Becton Dickinson) (14). Antibiotic susceptibilities were determined according to CLSI M100-S25.…”

Section: Methodsmentioning

confidence: 99%

Analysis of Clinical Isolates of Extended-Spectrum β-Lactamase-Producing Bacteria with Primer and Probe Sets Developed to Detect blaCTX-M, blaTEM, and blaSHV Using a Fully Automated Gene Detection System

et al. 2019

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Extended-Spectrum Beta-Lactamase Detection with Different Panels for Automated Susceptibility Testing and with a Chromogenic Medium

Cited by 9 publications

References 0 publications

Carbon screen‐printed electrodes on ceramic substrates for label‐free molecular detection of antibiotic resistance

Carbon screen‐printed electrodes on ceramic substrates for label‐free molecular detection of antibiotic resistance

Molecular characterization of extended-spectrum β-lactamase-producing Escherichia coli isolated from postpartum uterine infection in dairy cattle in India

Analysis of Clinical Isolates of Extended-Spectrum β-Lactamase-Producing Bacteria with Primer and Probe Sets Developed to Detect <i>bla</i><sub>CTX-M</sub>, <i>bla</i><sub>TEM</sub>, and <i>bla</i><sub>SHV</sub> Using a Fully Automated Gene Detection System

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