Extended monitoring of resistance to Bacillus thuringiensis Cry1Ab maize in Diatraea saccharalis (Lepidoptera

Huang, Fangneng; Ghimire, Mukti N.; Leonard, B. R.; Daves, C. A.; Levy, Ronnie; Baldwin, Jack

doi:10.4161/gmcr.20539

Cited by 47 publications

(44 citation statements)

References 29 publications

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“…The Cry1Ab-SS strain is susceptible to purified Cry1Aa, Cry1Ab, and Cry1Ac (Huang et al, 2012b), as well as to Bt maize plants expressing Cry1Ab, Cry1A.105, Cry2Ab2, and/or Cry1F (Wangila et al, 2012). Six Cry1Ab-maize resistant strains of D. saccharalis were developed from six out of the eight single-pair families that were identified to possess major resistance alleles to Cry1Ab maize in 2009 (Huang et al, 2012a). These eight single-pair families were selected using an F 2 screen from 191 feral individuals sampled from maize field in southeast Louisiana in 2009 in the same location as the Cry1Ab-SS strain was collected.…”

Section: Sources Of Cry1ab-susceptible and -Resistant Strains Of D Smentioning

confidence: 99%

“…The sugarcane borer, Diatraea saccharalis (F.), is a major maize borer pest in many areas of the mid-southern region of the United States and South America (PRNewswire, 2009;Huang et al, 2012a). Studies have shown that D. saccharalis is inherently less susceptible to Bt proteins than other major maize borer species such as European corn borer, Ostrinia nubilalis (Hübner), and southwestern corn borer, Diatraea grandiosella Dyar .…”

Section: Introductionmentioning

confidence: 99%

“…Both Wu et al (2007) and Ghimire et al (2011) also reported that most commercial Cry1Ab maize hybrids did not express a ''high dose'' of Bt proteins for D. saccharalis. To ensure the long-term success of Bt maize for managing stalk borers in the mid-southern region, since 2004, a cost-effective Bt resistance monitoring program has been implemented in the region (Huang et al, 2012a). By using an F 2 /F 1 screen, the monitoring program can detect rare resistance alleles to Cry1Ab maize in field populations of D. saccharalis.…”

Section: Introductionmentioning

confidence: 99%

“…By using an F 2 /F 1 screen, the monitoring program can detect rare resistance alleles to Cry1Ab maize in field populations of D. saccharalis. In 2009, a total of 110 F 2 families of D. saccharalis derived from 191 feral individuals sampled from maize fields in southeast Louisiana were examined for resistance to Cry1Ab maize using an F 2 screen (Huang et al, 2012a). Eight out of the 191 individuals were identified to possess major resistance alleles to Cry1Ab maize plants.…”

Section: Introductionmentioning

confidence: 99%

“…Eight out of the 191 individuals were identified to possess major resistance alleles to Cry1Ab maize plants. Larvae of these families were able to survive on whole plants of commercial Cry1Ab maize hybrids in the greenhouse (Huang et al, 2012a). Laboratory strains were established for six of the eight families.…”

Section: Introductionmentioning

confidence: 99%

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Susceptibility of Cry1Ab maize-resistant and -susceptible strains of sugarcane borer (Lepidoptera: Crambidae) to four individual Cry proteins

Zhang

Huang

Leonard

et al. 2013

Journal of Invertebrate Pathology

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a b s t r a c tSugarcane borer, Diatraea saccharalis (F.), is a major target of Bt maize in South America and many areas of the US mid-south region. Six laboratory strains of D. saccharalis were established from six single-pair F 2 families possessing major resistance alleles to Cry1Ab maize hybrids. Susceptibility of the six strains was evaluated on diet treated with each of four purified trypsin-activated Bt proteins, Cry1Ab, Cry1Aa, Cry1Ac and Cry1F. Bt susceptibility of the six strains was compared with that of known Cry1Ab-susceptible and -resistant strains of D. saccharalis. At least two of the six strains demonstrated a similar level (>526-fold) of resistance to Cry1Ab as shown in the known Cry1Ab-resistant strain, while resistance levels were relatively lower for other strains (116-to 129-fold). All the six strains were highly cross-resistant to Cry1Aa (71-to 292-fold) and Cry1Ac (30-to 248-fold), but only with a low level to Cry1F (<7-fold). Larval growth of all six strains was also inhibited on Bt-treated diet, but, except for Cry1F, the growth inhibition of the six strains was considerably less than that of the Cry1Ab-susceptible larvae. The results provide clear evidence that the observed resistance to Cry1Ab maize in the six strains is a result of resistance to the Cry1Ab protein in the plants. The low level of cross-resistance between Cry1A and Cry1F suggests that pyramiding these two types of Bt proteins into a plant could be a good strategy for managing D. saccharalis.

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Section: Sources Of Cry1ab-susceptible and -Resistant Strains Of D Smentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Susceptibility of Cry1Ab maize-resistant and -susceptible strains of sugarcane borer (Lepidoptera: Crambidae) to four individual Cry proteins

Zhang

Huang

Leonard

et al. 2013

Journal of Invertebrate Pathology

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Occurrence and larval movement of Diatraea saccharalis (Lepidoptera: Crambidae) in seed mixes of non-Bt and Bt pyramid corn

et al. 2013

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Varied frequencies of resistance alleles to Cry1Ab and Cry1Ac among Brazilian populations of the sugarcane borer, Diatraea saccharalis (F.)

Oliveira

Sakuno

Miraldo

et al. 2022

Pest Management Science

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BACKGROUND: Brazil is the largest grower of the world's 26 million ha of sugarcane, Saccharum officinarum. Pest damage mainly by the sugarcane borer, Diatraea saccharalis (F.), is a great challenge to the sugarcane industry. To control D. saccharalis, Brazil launched the world's first commercial use of Bt sugarcane in 2017. As part of the resistance management programs for Bt sugarcane planting, 535 F 2 isoline families of D. saccharalis collected from three major sugarcane planting states (Goiás, Minas Gerais and São Paulo) in Brazil during 2019-2020 were screened for resistance to two Bt sugarcane varieties: CTC20BT expressing Cry1Ab and CTC9001BT expressing Cry1Ac. Here we report the results of the first study related to Bt resistance in a sugarcane cropping system. RESULTS: Larval survivorships of these families in an F 2 screen on CTC20BT were highly correlated with their survival on CTC9001BT, whereas the Cry1Ac tissues exhibited greater insecticidal activities than Cry1Ab. Resistance allele frequencies (RAFs) for populations from Goiás and Minas Gerais were relatively low at 0.0034 for Cry1Ab and 0.0045 to Cry1Ac. By contrast, RAFs for São Paulo populations were considerably greater (0.0393 to Cry1Ab, 0.0245 to Cry1Ac).CONCLUSIONS: RAFs to Cry1Ab and Cry1Ac varied among Brazilian D. saccharalis populations. Prior selection resulting from an intensive use of single-gene Bt maize under low compliance of refuge planting could be a main factor contributing to the high RAF in São Paulo. The results suggest that mitigation measures including sufficient non-Bt maize refuge planting, effective resistance monitoring, and use of pyramided Bt sugarcane traits should be implemented promptly to prevent further increase in the RAF to ensure the sustainable use of Bt sugarcane in Brazil.

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Extended monitoring of resistance to Bacillus thuringiensis Cry1Ab maize in Diatraea saccharalis (Lepidoptera

Cited by 47 publications

References 29 publications

Susceptibility of Cry1Ab maize-resistant and -susceptible strains of sugarcane borer (Lepidoptera: Crambidae) to four individual Cry proteins

Susceptibility of Cry1Ab maize-resistant and -susceptible strains of sugarcane borer (Lepidoptera: Crambidae) to four individual Cry proteins

Occurrence and larval movement of Diatraea saccharalis (Lepidoptera: Crambidae) in seed mixes of non-Bt and Bt pyramid corn

Varied frequencies of resistance alleles to Cry1Ab and Cry1Ac among Brazilian populations of the sugarcane borer, Diatraea saccharalis (F.)

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