Extended-gate biosensors achieve fluid stability with no loss in charge sensitivity

Dak, Piyush; Nair, Pradeep R.; Go, Jonghyun; Alam, Muhammad A.

doi:10.1109/drc.2013.6633815

Cited by 7 publications

(6 citation statements)

References 3 publications

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“…This significantly simplifies device fabrication and reduces costs as readout chips are reusable, whilst the sensing chips can be designed as low-cost disposables. Furthermore, the sensor surface can be engineered independent of the transducer to achieve stable selective functionalization, retaining the charge sensitivity of conventional or nanomaterial-based ISFETs (Dak et al 2013;Tarasov et al 2015a). Potentiometric sensors have not been thoroughly explored as possible diagnostic tools for monitoring immunological responses to infections and whilst several proof-of-concept studies have investigated model antigen-antibody interactions using various types of FETs, the majority of this previous work was performed in buffer systems, and not actual blood samples (Mu et al 2015).…”

Section: Introductionmentioning

confidence: 99%

A potentiometric biosensor for rapid on-site disease diagnostics

Tarasov

Gray

Tsai

et al. 2016

Biosensors and Bioelectronics

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Quantitative point-of-care (POC) devices are the next generation for serological disease diagnosis. Whilst pathogen serology is typically performed by centralized laboratories using Enzyme-Linked ImmunoSorbent Assay (ELISA), faster on-site diagnosis would infer improved disease management and treatment decisions. Using the model pathogen Bovine Herpes Virus-1 (BHV-1) this study employs an extended-gate field-effect transistor (FET) for direct potentiometric serological diagnosis. BHV-1 is a major viral pathogen of Bovine Respiratory Disease (BRD), the leading cause of economic loss ($2 billion annually in the US only) to the cattle and dairy industry. To demonstrate the sensor capabilities as a diagnostic tool, BHV-1 viral protein gE was expressed and immobilized on the sensor surface to serve as a capture antigen for a BHV-1-specific antibody (anti-gE), produced in cattle in response to viral infection. The gE-coated immunosensor was shown to be highly sensitive and selective to anti-gE present in commercially available anti-BHV-1 antiserum and in real serum samples from cattle with results being in excellent agreement with Surface Plasmon Resonance (SPR) and ELISA. The FET sensor is significantly faster than ELISA (<10 min), a crucial factor for successful disease intervention. This sensor technology is versatile, amenable to multiplexing, easily integrated to POC devices, and has the potential to impact a wide range of human and animal diseases.

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Section: Introductionmentioning

confidence: 99%

A potentiometric biosensor for rapid on-site disease diagnostics

Tarasov

Gray

Tsai

et al. 2016

Biosensors and Bioelectronics

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“…Potentiometric biosensors, which detect the analyte charge directly, allow label-free detection and are easily miniaturized [ 36 , 37 , 38 , 39 , 40 ]. Since the target molecules conjugate with the probe molecules (usually immobilized on the sensor surface as shown in Figure 1 (c4), left) only in salt-based electrolyte solutions, screening by these ions fundamentally limits the sensitivity of charge-based (potentiometric) biosensors.…”

Section: Introductionmentioning

confidence: 99%

Droplet-based Biosensing for Lab-on-a-Chip, Open Microfluidics Platforms

et al. 2016

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Low cost, portable sensors can transform health care by bringing easily available diagnostic devices to low and middle income population, particularly in developing countries. Sample preparation, analyte handling and labeling are primary cost concerns for traditional lab-based diagnostic systems. Lab-on-a-chip (LoC) platforms based on droplet-based microfluidics promise to integrate and automate these complex and expensive laboratory procedures onto a single chip; the cost will be further reduced if label-free biosensors could be integrated onto the LoC platforms. Here, we review some recent developments of label-free, droplet-based biosensors, compatible with “open” digital microfluidic systems. These low-cost droplet-based biosensors overcome some of the fundamental limitations of the classical sensors, enabling timely diagnosis. We identify the key challenges that must be addressed to make these sensors commercially viable and summarize a number of promising research directions.

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“…ISFET-based integrated circuits have been developed for DNA sequencing [10] and for quantitative and digital Polymerase Chain Reaction (PCR) [11]. For instance, the Ion Torrent TM platform has been demonstrated down to the 110 nm CMOS technology node [12] and its further scalability has been investigated theoretically [13].…”

Section: Introductionmentioning

confidence: 99%

A TCAD-Based Methodology to Model the Site-Binding Charge at ISFET/Electrolyte Interfaces

Bandiziol

Palestri

Pittino

et al. 2015

IEEE Trans. Electron Devices

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We propose a new approach to describe in commercial TCAD the chemical reactions that occur at dielectric/electrolyte interface and make the ion sensitive FET (ISFET) sensitive to pH. The accuracy of the proposed method is successfully verified against the available experimental data.\ud We demonstrate the usefulness of the method by performing, for the first time in a commercial TCAD environment, a full 2-D analysis of ISFET operation, and a comparison between threshold voltage and drain current differential sensitivities in the linear and saturation regimes. The method paves the way to accurate\ud and efficient ISFET modeling with standard TCAD tools

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Extended-gate biosensors achieve fluid stability with no loss in charge sensitivity

Cited by 7 publications

References 3 publications

A potentiometric biosensor for rapid on-site disease diagnostics

A potentiometric biosensor for rapid on-site disease diagnostics

Droplet-based Biosensing for Lab-on-a-Chip, Open Microfluidics Platforms

A TCAD-Based Methodology to Model the Site-Binding Charge at ISFET/Electrolyte Interfaces

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