Extended Blood Group Molecular Typing and Next-Generation Sequencing

Liu, Zhugong; Liu, Meihong; Mercado, Teresita; Illoh, Orieji; Davey, R. B.

doi:10.1016/j.tmrv.2014.08.003

Cited by 39 publications

(44 citation statements)

References 113 publications

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“…16,17 Next-generation sequencing (NGS) has the potential to redress these limitations and is being implemented for HLA analysis in tissue typing reference laboratories. 18 Similarly, NGS is rapidly moving toward routine practice for patient and donor blood group typing, 19,20 yet a large worldwide survey of genomic blood group variation is lacking.…”

Section: Introductionmentioning

confidence: 99%

Erythrogene: a database for in-depth analysis of the extensive variation in 36 blood group systems in the 1000 Genomes Project

et al. 2016

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Section: Introductionmentioning

confidence: 99%

Erythrogene: a database for in-depth analysis of the extensive variation in 36 blood group systems in the 1000 Genomes Project

et al. 2016

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“…1 Blood transfusion requires a mandatory cross-match test to examine the compatibility between donor and recipient blood groups. The pioneering efforts of Karl Landsteiner on human blood groups, leads to a safe blood transfusiontoday 2,3 .The ABO blood group system was the first system described and remains the most significant in transfusion medicine. A mismatch of ABO may be fatal, whereas a mismatch of other blood groups initially mostly is harmless.…”

Section: Introductionmentioning

confidence: 99%

Frequency pattern of blood group systems among two healthy groups at albaha province, saudi arabia; a cross sectional, laboratory based study

Abukhelaif¹,

Alghamdi²,

Alghamdi³

et al. 2017

Int J Recent Sci Res

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ARTICLE INFO ABSTRACTObjective: This study was aimed to determine and document the frequency pattern of ABO and Rh blood groups among two healthy population at Albaha, Saudi Arabia. Method:The design used for this study was a retrospective, cross sectional, laboratory based study for the distribution of ABO blood group and Rh factor conducted at both Albaha Central Blood Bank and Faculty of Medicine, Albaha University, Saudi Arabia. The study population constitute 2356 subjects enrolled into the studyduring the years 2015 and 2016. While the second group were medical students studying at Faculty of medicine Albaha University, who were potential blood donors(270 participant) Data Collection: Data regarding frequency and distribution of ABO and Rh groups of the screened blood donors at the Central Blood Bank were retrieved from registration books from Jan 2015 up to December 2016 using prepared Checklists . A total of 2155 blood donors were screened within the study period Results: A total of 2425 study population were included over the study period from January 2015 to December 2016. Out of these (88.87%) were Blood Bank donors and (11.13 %) were medical students. Amongst ABO blood group system, the most common group was O (47.67%) followed by group A (33.57%), B (13.69%) and AB (5.07%). The overall frequency of RhD positive and RhD negative blood group among our population was found to be 90.88 % and 9.11% respectively, with equal rates in the two groups Conclusion: Frequency of "Rh-positive blood group" is A, O, AB and B, whereas the frequency of the most common Rh-negative blood group are B, AB,O, and A respectively. The determination of the frequency of blood groups in the region would not only help in blood transfusion services, but also reduce the risk of erythroblastosis foetalis in the neonates.

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“…Hybridization signals are then analyzed by measuring fluorescence intensities (usually), and a RBC phenotype is predicted based on the genotype result. 76–78 Predicting the phenotype from a genotype is relatively straightforward for many RBC antigens encoded by SNPs. However, there still remain some blood group systems where genotyping remains challenging because the genes encode enzymes involved in the modification of carbohydrate chains (e.g.…”

Section: Use Of Rbc Genotyping For Aiding In Transfusion Management Omentioning

confidence: 99%

“…81 In addition, other customizable high throughput genotyping platforms such as TaqMan® Open Array® (Applied Biosystems, Grand Island, New York) have also be used. 76 …”

Section: Use Of Rbc Genotyping For Aiding In Transfusion Management Omentioning

confidence: 99%

Genotyping Applications for Transplantation and Transfusion Management: The Emory Experience

Fasano¹,

Sullivan²,

Bray³

et al. 2017

Archives of Pathology &Amp; Laboratory Medicine

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Current genotyping methodologies for transplantation and transfusion management employ multiplex systems that allow for the simultaneous detection of multiple human leukocyte antigens (HLA), human platelet antigens (HPA) and red blood cell (RBC) antigens. The development of high resolution molecular HLA typing has led to improved outcomes of unrelated hematopoietic stem cell transplants by better identifying suitable donors typed at the allele level for HLA-A, B, C, DRB1 and DQB1 antigens. In solid organ transplantation, the combination of high resolution HLA typing along with solid-phase antibody identification and the calculated PRA have shown to be of specific benefit to highly sensitized patients, and have resulted in significant reductions of incompatible crossmatches at the time of organ allocation. This database-driven combined HLA antigen/antibody testing has promoted the routine implementation of the virtual crossmatch, in which an electronic crossmatch is performed, and perhaps even obviates the need for a physical crossmatch. Additionally, DNA-based testing for RBC antigens provides as an alternative typing method that mitigates many of the limitations of hemagglutination-based phenotyping. Although there are many applications of RBC genotyping in various transfusion settings, it has arguably been most useful in the management of transfusion-dependent patients with sickle cell disease (SCD) and thalassemia to minimize alloimmunization. The availability of high-throughput RBC genotyping for both patients and large populations of donors, along with coordinated informatics systems to link patients’ antigen needs with available antigen-negative and/or rare blood-typed donors, offer promise toward improving the efficiency, reliability, and extent of RBC matching for this population.

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Extended Blood Group Molecular Typing and Next-Generation Sequencing

Cited by 39 publications

References 113 publications

Erythrogene: a database for in-depth analysis of the extensive variation in 36 blood group systems in the 1000 Genomes Project

Erythrogene: a database for in-depth analysis of the extensive variation in 36 blood group systems in the 1000 Genomes Project

Frequency pattern of blood group systems among two healthy groups at albaha province, saudi arabia; a cross sectional, laboratory based study

Genotyping Applications for Transplantation and Transfusion Management: The Emory Experience

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