Exsheathment of Ostertagia ostertagi infective larvae following exposure to bovine rumen contents derived from low and high roughage diets

DeRosa, A.; Chirgwin, Sharon R.; Fletcher, J. L.; Williams, Justin; Klei, Thomas R.

doi:10.1016/j.vetpar.2004.12.019

Cited by 17 publications

(10 citation statements)

References 8 publications

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“…In particular, the results acquired by De Rosa et al (2005) showed differences in the rate of exsheathment of Ostertagia ostertagi larvae in calves fed on low or high roughage diets. Although the differences in experimental conditions (time of measurement, host and parasite species) make comparisons difficult, nonetheless the delay in the exsheathment process in the current study appeared much greater than that recorded by De Rosa et al (2005) and was particularly evident in the groups offered a high proportion of sainfoin. In calves, it has been proposed that the observed changes could be due to variations in ruminal pH related to the diet composition.…”

Section: Discussionmentioning

confidence: 94%

“…1985, 1986; Sommerville and Rogers, 1987; Petronijevic and Rogers, 1987). However, only a few recent studies have examined how the local conditions in the rumen, depending either on the host species (Hertzberg et al 2002) or on the host diet (De Rosa et al 2005) might modulate the exsheathment of larvae. In particular, the results acquired by De Rosa et al (2005) showed differences in the rate of exsheathment of Ostertagia ostertagi larvae in calves fed on low or high roughage diets.…”

Section: Discussionmentioning

confidence: 99%

“…However, only a few recent studies have examined how the local conditions in the rumen, depending either on the host species (Hertzberg et al 2002) or on the host diet (De Rosa et al 2005) might modulate the exsheathment of larvae. In particular, the results acquired by De Rosa et al (2005) showed differences in the rate of exsheathment of Ostertagia ostertagi larvae in calves fed on low or high roughage diets. Although the differences in experimental conditions (time of measurement, host and parasite species) make comparisons difficult, nonetheless the delay in the exsheathment process in the current study appeared much greater than that recorded by De Rosa et al (2005) and was particularly evident in the groups offered a high proportion of sainfoin.…”

Section: Discussionmentioning

confidence: 99%

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The kinetics of exsheathment of infective nematode larvae is disturbed in the presence of a tannin-rich plant extract (sainfoin) both in vitro and in vivo

et al. 2007

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The mode of action of bioactive plants on gastrointestinal nematodes remains obscure. Previous in vitro studies showed that exsheathment was significantly disturbed after contact with tannin-rich extracts. However, the role of important factors (extract concentration, parasite species) has not been assessed and no information is available on the occurrence in vivo. These questions represent the objectives of this study. The model incorporated the parasites Haemonchus contortus and Trichostrongylus colubriformis with sainfoin as the bioactive plant. A set of in vitro assays was performed, measuring the changes observed, after 3 h of contact with increasing concentrations of sainfoin, on the rate of artificial exsheathment. The results indicated that sainfoin extracts interfered with exsheathment in a dose-dependent manner and the process overall was similar for both nematodes. The restoration of control values observed after adding PEG to extracts confirms a major role for tannins. A second study was performed in vivo on rumen-cannulated sheep fed with different proportions of sainfoin in the diet to verify these in vitro results. The consumption of a higher proportion of sainfoin was indeed associated with significant delays in Haemonchus exsheathment. Overall, the results confirmed that interference with the early step of nematode infection might be one of the modes of action that contributes to the anthelmintic properties of tanniniferous plants.

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Section: Discussionmentioning

confidence: 94%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

The kinetics of exsheathment of infective nematode larvae is disturbed in the presence of a tannin-rich plant extract (sainfoin) both in vitro and in vivo

et al. 2007

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“…The rumen environment and the chemical nature of the consumed material can be altered as forages and their biological compounds are digested (Rogosic et al, 2003;Patra and Saxena, 2009), which can potentially affect ingested larvae (Howell et al, 1999;DeRosa et al, 2005;Brunet et al, 2007). Therefore, creating an in vitro rumen environment where quantity of forage material representing true in vivo conditions can be included, and solution from the digested material can be used to rapidly evaluate larvae motility is important.…”

Section: Introductionmentioning

confidence: 99%

A modified in vitro larvae migration inhibition assay using rumen fluid to evaluate Haemonchus contortus viability

Whitney¹,

Lee²,

Klein

et al. 2011

Veterinary Parasitology

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“…In vitro and in vivo studies have indicated that the bicarbonate/carbonic acid balance (Petronijevic, Rogers andSomerville, 1985, Sciacca et al 2002) and the pH (Hertzberg et al 2002, Davey andRogers, 1982) of the luminal content were major factors to stimulate the exsheathment. Recent in vivo results have indicated that the diet composition could also modulate the process by affecting these factors (DeRosa et al 2005).…”

mentioning

confidence: 99%

Effects of four tanniferous plant extracts on thein vitroexsheathment of third-stage larvae of parasitic nematodes

Bahuaud¹,

Montellano

Chauveau³

et al. 2006

Parasitology

104

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The anthelmintic properties of tanniferous plants and of their secondary metabolites represent one possible alternative to chemotherapy that is currently being explored as a means of achieving sustainable control of gastrointestinal nematodes in ruminants. Previous in vivo and in vitro results suggest that tanniferous plants can have direct anti-parasitic effect against different stages of nematodes. However, the mode of action of the bioactive plant compounds remains obscure. The objectives of the current study were (1) to examine the hypothesis that extracts of tanniferous plants might interfere with the exsheathment of third-stage infective larvae (L3); (2) to assess the role of tannins in the process by examining the consequence of adding an inhibitor of tannins (polyethylene glycol: PEG) to extracts. The effects of 4 tanniferous plant extracts on exsheathment have been examined on L3 of Haemonchus contortus and Trichostrongylus colubriformis. Artificial exsheathment was induced in vitro by adding hypochloride solution to larval suspension. The evolution of exsheathment with time was measured by repeated observations at 10-min interval for 60 min. The selected plants were: genista (Sarothamnus scoparius), heather (Erica erigena), pine tree (Pinus sylvestris), and chestnut tree (Castanea sativa), with tannin contents ranging from 1.5 to 24.7% of DM. Extracts of a non-tanniferous plant (rye grass, tannin content: 0.3% of DM) were included in the assay as negative controls. The extracts were tested at the concentration of 600 microg/ml and the effects were compared to the rate of exsheathment of control larvae in PBS. No statistical differences in the pattern of exsheathment was observed after addition of rye grass or genista extracts for both nematode species and with heather extracts for T. colubriformis. In contrast, pine tree extracts on larvae of both species and heather extracts with H. contortus induced a significant delay in exsheathment. Last, contact with chest nut extracts led to a total inhibition of the process for both nematodes. These results suggest that extracts of tanniferous plants might affect a key process in the very early stages of larval invasion of the host. In most cases, the addition of PEG led to a total or partial restoration towards control values. This suggests that tannins are largely involved in the inhibitory process. However, other secondary metabolites may also interfere with the process that would help to explain some of the differences in response observed between the two nematode species.

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Exsheathment of Ostertagia ostertagi infective larvae following exposure to bovine rumen contents derived from low and high roughage diets

Cited by 17 publications

References 8 publications

The kinetics of exsheathment of infective nematode larvae is disturbed in the presence of a tannin-rich plant extract (sainfoin) both in vitro and in vivo

The kinetics of exsheathment of infective nematode larvae is disturbed in the presence of a tannin-rich plant extract (sainfoin) both in vitro and in vivo

A modified in vitro larvae migration inhibition assay using rumen fluid to evaluate Haemonchus contortus viability

Effects of four tanniferous plant extracts on thein vitroexsheathment of third-stage larvae of parasitic nematodes

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