Expressions of a calcium-binding protein (spot35/calbind in-D28k) in mouse olfactory cells: Possible relationship to neuronal differentiation

Fujiwara, Mitsuru; Nakamura, Hideo; Kawasaki, M.; Nakano, Yuichi; Kuwano, Ryozo

doi:10.1007/bf01526190

Cited by 13 publications

(9 citation statements)

References 19 publications

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“…In our present study, we observed postnatal changes in dividing cells by using PCNA antiserum immunohistochemical staining [5,13]. The dividing cells varied markedly in number and arrangement during development.…”

Section: Discussionmentioning

confidence: 67%

Age-related changes in dividing cells of the olfactory epithelium of the maturing guinea pig

Nakamura

Fujiwara

Kawasaki

et al. 1998

European Archives of Oto-Rhino-Laryngology

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Changes in dividing cells of the olfactory epithelium from guinea pigs of different ages were examined by immunohistochemical staining using anti-proliferating cell nuclear antigen antibody. Numerous dividing cells were scattered diffusely in the basal layer of the olfactory epithelium at 1 and 2 months following birth and then gradually decreased with maturation until 4 months. Findings then remained constant between 4 and 24 months. Subsequently, cell numbers were found to decrease as animals became older. The number of olfactory receptor cells did not vary significantly between 1 and 30 months. Although no correlation could be found between the numbers of dividing cells and olfactory receptor cells, it is still possible that the longevity of the olfactory receptor cells changes to maintain the overall size of the neuronal population.

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Section: Discussionmentioning

confidence: 67%

Age-related changes in dividing cells of the olfactory epithelium of the maturing guinea pig

Nakamura

Fujiwara

Kawasaki

et al. 1998

European Archives of Oto-Rhino-Laryngology

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“…Transcriptomic analysis of the Prokr2+ Pioneer/TN cells revealed an enriched expression of the putative Grueneberg ganglia marker Pde2a (Zhi Huang, 2018), Pcsk1n, Tmem130, and Resp18. In addition to these, in the Migratory Prokr2+ cells, we identified the expression of several previously reported antigens for the putative terminal nerve/pioneer neurons, such as Calbindin1 (Fujiwara et al, 1997) (Fig. 9), Lhx2 (Palaniappan et al, 2019) (Fig.…”

Section: Discussionmentioning

confidence: 81%

Illuminating the Terminal Nerve: Uncovering the Link between GnRH-1 and Olfactory Development

Amato,

Taroc,

Forni

2023

Preprint

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During embryonic development, the olfactory placode (OP) generates migratory neurons, including olfactory pioneer neurons, cells of the terminal nerve (TN), Gonadotropin-releasing hormone-1 (GnRH-1) neurons, and other uncharacterized neurons. Pioneer neurons from the olfactory placode induce olfactory bulb morphogenesis.In mice, GnRH-1 neurons appear in the olfactory system around mid-gestation and migrate via the terminal nerve axons to different brain regions. The GnRH-1 neurons are crucial in controlling the hypothalamic-pituitary-gonadal (HPG) axis. Kallmann syndrome is characterized by impaired olfactory system development, defective olfactory bulbs, defective secretion of GnRH-1, and infertility. The precise mechanistic link between the olfactory system and GnRH-1 development remains unclear. Studies in humans and mice highlight the importance of the Prokineticin-2/Prokineticin-Receptor-2 (Prokr2) signaling pathway in olfactory bulb morphogenesis and GnRH-1 neuronal migration.Prokr2loss-of-function mutations can cause Kallmann syndrome, and hence the Prokr2 signaling pathway represents a unique model to decipher the olfactory/GnRH-1 connection. We discovered that Prokr2 is expressed in the TN neurons during the critical period of GnRH-1 neuron formation, migration, and induction of olfactory bulb morphogenesis. Single-cell RNA sequencing identified that the TN is formed by neurons that are distinct from the olfactory neurons. The TN neurons express multiple genes associated with KS. Our study suggests that the aberrant development of pioneer/TN neurons might cause the KS spectrum.Key Points1) Pioneer or terminal nerve neurons play a crucial role in initiating the development of the olfactory bulbs. We found that the Prokineticin Receptor-2 gene, associated with Kallmann syndrome, is expressed by the olfactory pioneer/terminal nerve neurons.2) We genetically traced, isolated, and conducted Single-cell RNA sequencing on terminal nerve neurons of rodents. This analysis revealed a significant enrichment of gene expression related to Kallmann syndrome.3) Our study indicates that the investigation of Pioneer/terminal nerve neurons should be a pivotal focal point for comprehending developmental defects affecting olfactory and GnRH-1 systems.

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“…In D. rerio , while S100 CBP was observed in crypt ORNs, CR widely occurred in ORNs (Castro et al., 2006; Germanà et al., 2007). Although scgn has not been reported in the ORNs of other vertebrate species, development‐stage dependent expression of CB, CR, calmodulin, and neurocalcin was observed in rodent ORNs (Bastianelli et al., 1995; Fujiwara, et al., 1997). Expression of an EF‐hand calcium sensor, visinin‐like protein (VILIP), was observed in the olfactory sensory cells of rat (Boekhoff et al., 1997).…”

Section: Discussionmentioning

confidence: 99%

Secretagogin in the brain and pituitary of the catfish, Clarias batrachus: Molecular characterization and regulation by insulin

Basu

Mitra

Singh

et al. 2022

J of Comparative Neurology

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Secretagogin (scgn), is a novel hexa EF-hand, phylogenetically conserved calciumbinding protein. It serves as Ca 2+ sensor and participates in Ca 2+ -signaling and neuroendocrine regulation in mammals. However, its relevance in the brain of nonmammalian vertebrates has largely remained unexplored. To address this issue, we studied the cDNA encoding scgn, scgn mRNA expression, and distribution of scgnequipped elements in the brain and pituitary of a teleost, Clarias batrachus (cb). The cbscgn cDNA consists of three transcripts (T) variants: T1 (2185 bp), T2 (2151 bp) and T3 (2060 bp). While 816 bp ORF in T1 and T2 encodes highly conserved six EF-hand 272 aa protein fully capable of Ca 2+ -binding, 726-bp ORF in T3 encodes 242 aa protein.The T1 showed >90% and >70% identity with scgn of catfishes, and other teleosts and mammals, respectively. The T1-mRNA was widely expressed in the brain and pituitary, while the expression of T3 was restricted to the telencephalon. Application of the antiscgn antiserum revealed a ∼32 kDa scgn-immunoreactive (scgn-i) band (known molecular weight of scgn) in the forebrain tissue, and immunohistochemically labeled neurons in the olfactory epithelium and bulb, telencephalon, preoptic area, hypothalamus, thalamus, and hindbrain. In the pituitary, scgn-i cells were seen in the pars distalis and intermedia. Insulin is reported to regulate scgn mRNA in the mammalian hippocampus, and feeding-related neuropeptides in the telencephalon of teleost. Intracranial injection of insulin significantly increased T1-mRNA expression and scgn-immunoreactivity

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Expressions of a calcium-binding protein (spot35/calbind in-D28k) in mouse olfactory cells: Possible relationship to neuronal differentiation

Cited by 13 publications

References 19 publications

Age-related changes in dividing cells of the olfactory epithelium of the maturing guinea pig

Age-related changes in dividing cells of the olfactory epithelium of the maturing guinea pig

Illuminating the Terminal Nerve: Uncovering the Link between GnRH-1 and Olfactory Development

Secretagogin in the brain and pituitary of the catfish, Clarias batrachus: Molecular characterization and regulation by insulin

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