Age-related changes in dividing cells of the olfactory epithelium of the maturing guinea pig

Nakamura, Hideo; Fujiwara, Michio; Kawasaki, Maiko; Nonomura, Naobumi; Takahashi, Sugata

doi:10.1007/s004050050061

Cited by 20 publications

(20 citation statements)

References 13 publications

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“…Mitotic activity of OE cells during postnatal development has been demonstrated in other species including guinea pigs (Nakamura et al, 1998;Higuchi et al, 2005), mice (Ohta and Ichimura, 2000), and rats Farbman, 1997, 1998) with a similar finding that the rate of cell proliferation decreases postnatally. In the rat OE (Weiler and Farbman, 1998), 5-bromo-2 0 -deoxyuridine (BrdU)-labeling index for the basal cell population was shown to decreased from a high of 30% at postnatal day 1 to a low of 5% at postnatal day 181.…”

Section: Postnatal Morphology Of Olfactory Mucosa In Rabbitsmentioning

confidence: 52%

Morphofunctional Adaptations of the Olfactory Mucosa in Postnatally Developing Rabbits

Kavoi

Makanya

Plendl

et al. 2012

The Anatomical Record

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Rabbits are born blind and deaf and receive unusually limited maternal care. Consequently, their suckling young heavily rely on the olfactory cue for nipple attachment. However, the postnatal morphofunctional adaptations of olfactory mucosa (OM) are not fully elucidated. To clarify on the extent and the pattern of refinement of the OM following birth in the rabbit, morphologic and morphometric analysis of the mucosa were done at neonatal (0-1 days), suckling (2 weeks), weanling (4 weeks), and adult (6-8 months) stages of postnatal development. In all the age groups, the basic components of the OM were present. However, proliferative activity of cells of the mucosal epithelium decreased with increasing age as revealed by Ki-67 immunostaining. Diameters of axon bundles, packing densities of olfactory cells, and cilia numbers per olfactory cell knob increased progressively with age being 5.5, 2.1, and 2.6 times, respectively, in the adult as compared with the neonate. Volume fraction values for the bundles increased by 5.3% from birth to suckling age and by 7.4% from weaning to adulthood and the bundle cores were infiltrated with blood capillaries in all ages except in the adult where such vessels were lacking. The pattern of cilia projection from olfactory cell knobs also showed age-related variations, that is, arose as a tuft from the tips of the knobs in neonates and sucklings and in a radial pattern from the knob bases in weanlings and adults. These morphological changes may be attributed to the high olfactory functional demand associated with postnatal development in the rabbit.

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Section: Postnatal Morphology Of Olfactory Mucosa In Rabbitsmentioning

confidence: 52%

Morphofunctional Adaptations of the Olfactory Mucosa in Postnatally Developing Rabbits

Kavoi

Makanya

Plendl

et al. 2012

The Anatomical Record

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“…The ultrastructural analysis demonstrated morpholo-gical similarity between basal cells and those present in the middle layer and sustentacular epithelium as was described by Caggiano et al (1994) and Huard et al (1998). In addition, according to Nakamura et al (1998) and Higuchi et al (2005), sustentacular cells and olfactory neurons could be characterized by the presence of communicant gap junctions. Besides, numerous mitochondrias disperse evenly in the cytoplasm of neurons, as well as chromatin and euchromatin in the cytoplasm of basal cells.…”

Section: Pcna and Ki67 Immunostaining Of Cells From Oe Of 60-day Agedmentioning

confidence: 80%

Establishment of a protocol for obtention of neuronal stem cells lineages from the dog olfactory epithelium

et al. 2010

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Miglino M.A. 2010. Establishment of a protocol for obtention of neuronal stem cells lineages from the dog olfactory epithelium. Pesquisa . Departamento de Ciência Animal, Campus Universitário, Bairro Cibrazen, Bom Jesus, PI 64900-000, Brazil. E-mail: flavioribeiro@ufpi.edu.br A morphological and cell culture study from nasal mucosa of dogs was performed in order to establish a protocol to obtain a cell population committed to neuronal lineage, as a proposal for the treatment of traumatic and degenerative lesions in these animals, so that in the future these results could be applied to the human species. Twelve mongrel dogs of 60-day aged pregnancy were collected from urban pound dogs in São Paulo. Tissue from cribriform ethmoidal lamina of the fetuses was collected at necropsy under sterile conditions around 1h to 2h postmortem by uterine sections and sections from the fetal regions described above. Isolated cells of this tissue were added in DMEM/F-12 medium under standard conditions of incubation (5% CO 2 , >37°C). Cell culture based on isolated cells from biopsies of the olfactory epithelium showed rapid growth when cultured for 24 hours, showing phase-bright sphere cells found floating around the fragments, attached on culture flasks. After 20 days, a specific type of cells, predominantly ellipsoids or fusiform cells was characterized in vitro. The indirect immunofluorescence examination showed cells expressing markers of neuronal precursors (GFAP, neurofilament, oligodendrocyte, and III â-tubulin). The cell proliferation index showed Ki67 immunostaining with a trend to label cell groups throughout the apical region, while PCNA immunostaining label predominantly cell groups lying above the basal lamina. The transmission electron microscopy from the olfactory epithelium of dogs revealed cells with electron-dense cytoplasm and preserving the same distribution as those of positive cell staining for PCNA. Metabolic activity was confirmed by presence of euchromatin in the greatest part of cells. All these aspects give subsidies to support the hypothesis about resident progenitor cells among the basal cells of the olfactory epithelium, committed to renewal of these cell populations, especially neurons. RESUMO.-[Estabelecimento de um protocolo para obtenção de linhagens de células-tronco neuronais a partir do epitélio olfatório de cães.] Foi realizado um estudo morfológico e por cultivo celular a partir de células provenientes da mucosa olfatória de cães, como forma de estabelecer um protocolo de cultivo, como uma proposta para o tratamento de lesões traumáticas e nervosas degenerativas nestes animais e futuramente, para que tais resultados possam ser aplicados a espécie humana. Foram utilizados doze cães sem raça definida, a termo, oriundos de castrações do Centro de Controle de Zoonoses de São Paulo. O tecido da lâmina cribiforme do etmóide dos fetos foi coletado sob necropsia, em condições estéreis, 1 a 2 horas post mortem, por meio de incisão uterina e acesso da região fetal supracitada. Depois as células isol...

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“…A característica pseudoestratificada deste epitélio também possibilitou a observação de núcleos de cé-lulas marcados positivamente, em diversas camadas celulares (Fig.1b,1c). Essa observação encontrou-se alicerçada nas hipóteses da localização das sub-populações de células-tronco residentes nas porções média e basal do epitélio olfatório, como também foi descrito por Nakamura et al (1998) e Higuchi et al (2005, em estudos da dinâmica dos precursores celulares do epitélio olfatório em Cavia sp. jovens.…”

Section: Resul Resul Resul Resul Results T T T Tados E Discus Ados E Dunclassified

Dinâmica dos precursores celulares do epitélio olfatório de cães sem raça definida: um estudo imunohistoquímico e ultra-estrutural

et al. 2007

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O epitélio olfatório apresenta um mecanismo de diferenciação em que células-tronco dão origem a células progenitoras amplificadoras, as quais expressam um gene pró-neural denominado Mammalian Achaete Scute Homolog 1 (Mash1). Estas células podem se diferenciar em receptores olfatórios. O epitélio olfatório de cães sem raça definida (3 machos de um ano e 2 fêmeas de três de idade) foi analisado por imunolocalização do antígeno nuclear de proliferação celular (PCNA) e por microscopia eletrônica de transmissão. Verificou-se marcação positiva para PCNA em células do epitélio olfatório, particularmente acima da linha da membrana basal. A ultra-estrutura do epitélio olfatório revelou células adjacentes à lâmina basal, cuja eletrodensidade assemelha-se àquelas presentes no epitélio de sustentação, reforçando a idéia da renovação das células de sustentação e dos neurônios olfatórios locais. O epitélio olfatório é composto células basais, comprometidas com sua renovação, caracterizadas através da intensa atividade mitótica, identificada pela reação positiva ao PCNA. Estes resultados sugerem que há reposição das células sustentaculares locais e do sistema através de mecanismos semelhantes.

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Age-related changes in dividing cells of the olfactory epithelium of the maturing guinea pig

Cited by 20 publications

References 13 publications

Morphofunctional Adaptations of the Olfactory Mucosa in Postnatally Developing Rabbits

Morphofunctional Adaptations of the Olfactory Mucosa in Postnatally Developing Rabbits

Establishment of a protocol for obtention of neuronal stem cells lineages from the dog olfactory epithelium

Dinâmica dos precursores celulares do epitélio olfatório de cães sem raça definida: um estudo imunohistoquímico e ultra-estrutural

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