Expression Profiling of Strawberry Allergen Fra a during Fruit Ripening Controlled by Exogenous Auxin

Ishibashi, Misaki; Yoshikawa, Hiroki; Uno, Yuichi

doi:10.3390/ijms18061186

Cited by 11 publications

(6 citation statements)

References 42 publications

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“…In the previous research, the transcript level of Fra a 1.01 was correlated with maturing stage; the younger fruits expressed a higher amount of mRNA (Ishibashi et al, 2017;Petriccione et al, 2017). Meanwhile, the Fra a 1.01 protein accumulation level was constant during the ripening stages (Ishibashi et al, 2017). This tendency was consistent with the results of unshaded fruits at unripe stages (Figs.…”

supporting

confidence: 88%

“…The strawberry fruits were characterized by measuring size, fresh weight, and color as described by Ishibashi et al (2017). Fruit color was quantified by using a Handy Spectrophotometer NF 333 (NIPPON DENSHOKU, Tokyo, Japan) and scored by the L*, a* and b* values.…”

Section: Characterization Of Fruitsmentioning

confidence: 99%

“…Previous studies have revealed that there are two putative low temperature responsive elements in the promoter region of gene07080, a Fra a 1.01 ortholog in F. vesca (Alison Dunn et al, 1998;Ishibashi et al, 2018), and one element in the Fra a 1.01 promoter in F. × ananassa (unpublished data). Additionally, the transcript levels of Fra a 1.01 were found to be high during the green stage and relatively low during the red stage (Ishibashi et al, 2017;Petriccione et al, 2017). We considered that the accumulation levels of Fra a 1.01 in winter may depend on regulation by cold stress at the early ripening stage.…”

Section: Effects Of Low Temperature On Fra a 1 Expressionmentioning

confidence: 99%

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Seasonal Variation of the Major Allergen Fra a 1 in Strawberry Fruit

et al. 2019

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Strawberry (Fragaria × ananassa) contains a major allergen, Fra a 1, which causes oral allergic syndrome. Fra a 1 is a PR-10 homolog that is regulated by environmental conditions. The allergenicity of fruit caused by Fra a 1 may depend on the genotype or growing conditions. We analyzed the Fra a 1.01 transcript levels and Fra a 1.01 protein levels in strawberry fruits of several genotypes across all seasons. In the preliminary rough screening, we selected the line WH1 bearing white fruit and the red-fruited cultivar 'Akihime'. Under the same environmental conditions, there was no significant difference in Fra a 1.01 levels between the two cultivars over several months, suggesting that receptacle color was not indicative of allergenicity caused by Fra a 1.01. Fruits cultivated under the same environmental conditions should be used for comparisons of the allergenicity among genotypes. Both 'Akihime' and WH1 accumulated significantly higher levels of Fra a 1.01 protein in winter than in spring. We investigated the effects of irradiation and low temperature as environmental factors controlling the accumulation of Fra a 1.01 in winter. A shading treatment on fruit did not significantly affect Fra a 1.01 protein accumulation in strawberry fruits. Regarding variations over time, the Fra a 1.01 protein content was higher in fruits harvested at midnight in January than in those harvested at other times and in other months. These findings suggested that the Fra a 1.01 protein accumulates in response to environmental factors such as cold stress.

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confidence: 88%

Section: Characterization Of Fruitsmentioning

confidence: 99%

Section: Effects Of Low Temperature On Fra a 1 Expressionmentioning

confidence: 99%

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Seasonal Variation of the Major Allergen Fra a 1 in Strawberry Fruit

et al. 2019

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“…For instance, methyl jasmonate (MeJA)-treated strawberries showed a higher redness of fruit skin and anthocyanin content with an upregulation of FaUFGT and FaANS genes ( 30 ). Exogenous arginine treatment can inhibit the ripening and coloration of postharvest strawberry fruit ( 33 ). Some other plant hormones, such as abscisic acid and auxin, are involved in the process of fruit coloration ( 31 , 34 ).…”

Section: Introductionmentioning

confidence: 99%

Exogenous Ethylene Promotes Peel Color Transformation by Regulating the Degradation of Chlorophyll and Synthesis of Anthocyanin in Postharvest Mango Fruit

Chen

Wang

et al. 2022

Front. Nutr.

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Due to geographical location and climatic factors, postharvest storage and preservation of tropical fruits and vegetables are still facing huge challenges. Ethephon (ETH) is widely used as an ethylene donor to achieve the commercial color and flavor of climacteric fruits. However, the effect of ETH on fruit coloration was affected by many factors, such as fruit species, plant hormones, and storage conditions. In this study, the main mango variety “Guifei” in Hainan, China, was used to study the effects of different concentrations of ETH on fruit ripening and coloration during storage at 25°C. Results showed that postharvest treatment with ETH (300, 500, and 900 mg·L−1) enhanced the activities of ACS and ACO, stimulated the release of endogenous ethylene, and accelerated fruit softening and color transformation. Compared with control, ETH treatment not only accelerated the breakdown of chlorophyll with higher activities of Chlase and MDCase but also induced the synthesis of carotenoid and anthocyanin with higher activities of PAL, CHI, DFR, and UFGT. Moreover, the changes in DFR and UFGT activities coincided with the increase in ETH concentration. Further, correlation analysis showed that the production of endogenous ethylene induced by ETH was significantly negatively correlated with firmness and chlorophyll content, whereas positively correlated with MDA content and anthocyanin content. This study suggests that the positive effect of ETH on “Guifei” mango color transformation is concentration-dependent within a certain concentration range. Anthocyanin is the main pigment for the red formation of “Guifei” mango, and DFR and UFGT may play critical roles in anthocyanin synthesis. ETH promoted the red coloration by promoting the release of endogenous ethylene and enhancing the activities of anthocyanin synthesis enzymes.

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“…This finding suggests that auxin and GA might be first synthesized and accumulated in the fertilized ovaries and then be transported to receptacles and promote fruit enlargement (Kang et al, 2013 ). Thus, receptacles fail to expand after removing the attached achenes (Nitsch, 1950 ; Ishibashi et al, 2017 ). The mutation of FvRGA1 leads to the parthenocarpy of strawberry (Zhou et al, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

Auxin Coordinates Achene and Receptacle Development During Fruit Initiation in Fragaria vesca

et al. 2022

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In strawberries, fruit set is considered as the transition from the quiescent ovary to a rapidly growing fruit. Auxin, which is produced from the fertilized ovule in the achenes, plays a key role in promoting the enlargement of receptacles. However, detailed regulatory mechanisms for fruit set and the mutual regulation between achenes and receptacles are largely unknown. In this study, we found that pollination promoted fruit development (both achene and receptacle), which could be stimulated by exogenous auxin treatment. Interestingly, auxin was highly accumulated in achenes, but not in receptacles, after pollination. Further transcriptome analysis showed that only a small portion of the differentially expressed genes induced by pollination overlapped with those by exogenous auxin treatment. Auxin, but not pollination, was able to activate the expression of growth-related genes, especially in receptacles, which resulted in fast growth. Meanwhile, those genes involved in the pathways of other hormones, such as GA and cytokinin, were also regulated by exogenous auxin treatment, but not pollination. This suggested that pollination was not able to activate auxin responses in receptacles but produced auxin in fertilized achenes, and then auxin might be able to transport or transduce from achenes to receptacles and promote fast fruit growth at the early stage of fruit initiation. Our work revealed a potential coordination between achenes and receptacles during fruit set, and auxin might be a key coordinator.

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Expression Profiling of Strawberry Allergen Fra a during Fruit Ripening Controlled by Exogenous Auxin

Cited by 11 publications

References 42 publications

Seasonal Variation of the Major Allergen Fra a 1 in Strawberry Fruit

Seasonal Variation of the Major Allergen Fra a 1 in Strawberry Fruit

Exogenous Ethylene Promotes Peel Color Transformation by Regulating the Degradation of Chlorophyll and Synthesis of Anthocyanin in Postharvest Mango Fruit

Auxin Coordinates Achene and Receptacle Development During Fruit Initiation in Fragaria vesca

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