Expression profiling of long noncoding <scp>RNA</scp> identifies lnc‐<scp>MMP</scp>3‐1 as a prognostic biomarker in external auditory canal squamous cell carcinoma

Liu, Hong; Dai, Chenyang; Wu, Qianru; Liu, Hongyan; Li, Feitian

doi:10.1002/cam4.1213

Cited by 14 publications

(12 citation statements)

References 29 publications

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“…. FISH was conducted using the Ribo™ Fluorescent In Situ Hybridization kit (Guangzhou RiboBio co., Ltd.) as previously described (26). The TUSc8 probe was designed and synthesized by Guangzhou RiboBio co., Ltd. dAPI (Guangzhou RiboBio co., Ltd.) was used to stain the nuclei.…”

Section: Fluorescence In Situ Hybridization (Fish)mentioning

confidence: 99%

TUSC8 inhibits the development of osteosarcoma by sponging miR‑197‑3p and targeting EHD2

et al. 2020

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Osteosarcoma (OS) is one of the most common malignant bone tumours and generally occurs in children and adolescents. Increasing evidence has demonstrated that dysregulated long non-coding RNAs (lncRNAs) play crucial roles in the progression of various human neoplasms. Among these, tumour suppressor candidate 8 (TUSc8) is a novel lncRNA and has been reported to function as a tumour suppressor in cervical cancer. However, the exact role of TUSc8 in OS remains largely unknown. In the present study, it was observed that TUSc8 was markedly downregulated in OS tissues and cell lines. Functional experiments demonstrated that the overexpression of TUSC8 significantly suppressed the proliferation, migration, invasion and epithelial-mesenchymal transition (EMT), whereas it accelerated the apoptosis of OS cells. Mechanistically, TUSc8 served as a sponge for miR-197-3p, and EH-domain containing 2 (EHd2) was identified as a downstream target molecule of miR-197-3p. Further investigations indicated that EHD2 knockdown significantly reversed the effects on OS cellular processes induced by TUSC8 overexpression. On the whole, these findings indicate that TUSc8 functions as a competing endogenous RNA (ceRNA) to suppress OS cell growth and EMT via the miR-197-3p/EHd2 axis. TUSc8 may thus function as a potential therapeutic target in OS treatment.

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Section: Fluorescence In Situ Hybridization (Fish)mentioning

confidence: 99%

TUSC8 inhibits the development of osteosarcoma by sponging miR‑197‑3p and targeting EHD2

et al. 2020

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“…FISH was carried out by using RiboTM Fluorescent In Situ Hybridization Kit (RiboBio, Guangzhou, China) and procedures were performed based on a previous study 30 . The LINC00518 probe was designed and synthesized by RiboBio (Guangzhou, China).…”

Section: Methodsmentioning

confidence: 99%

Long noncoding RNA LINC00518 acts as a competing endogenous RNA to promote the metastasis of malignant melanoma via miR-204-5p/AP1S2 axis

Luan

Ding

et al. 2019

Cell Death Dis

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Long intergenic nonprotein coding RNA 518 (LINC00518) has been shown to promote cancer cell growth and metastasis in some human tumors. Although it has been reported that LINC00518 is dysregulated in melanoma, its exact role and molecular mechanism in melanoma remain unclear. RNA-seq analysis and qRT-PCR was used to detect the expression of LINC00518 in melanoma tissues. Melanoma cases from The Cancer Genome Atlas (TCGA), GEO#GSE15605 and GEO#GSE24469 were included in this study. 3D migration, transwell and scratch wound assay were used to explore the role of LINC00518 in melanoma cells. Bioinformatics, luciferase reporter assays, MS2-RIP assay, RNA pull-down assay and RNA-ChIP assay were used to demonstrate the mechanism of LINC00518 in melanoma. We found that LICN00518 was significantly upregulated in melanoma tissue, and high LICN00518 level was an independent risk factor for melanoma patients. LICN00518 promoted the invasion and migration of melanoma cells. LICN00518 exerted its role by decoying miR-204-5p to upregulate Adaptor Related Protein Complex 1 Sigma 2 Subunit (AP1S2) expression. We also demonstrated that LICN00518 promoted melanoma metastasis in vivo through pulmonary metastasis assay. This result elucidates a new mechanism for LICN00518 in the metastasis of melanoma. LICN00518 may serve as a survival indicator and potential therapeutic target in melanoma patients.

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“…RiboTM Fluorescent In Situ Hybridization Kit (RiboBio, Guangzhou, China) was used to for FISH. The procedure was carried out according to the previous study [25]. The probe of LINC00520 was synthesized by RiboBio (Guangzhou, China).…”

Section: Fluorescence In Situ Hybridization (Fish)mentioning

confidence: 99%

Long non-coding RNA LINC00520 promotes the proliferation and metastasis of malignant melanoma by inducing the miR-125b-5p/EIF5A2 axis

Luan

Ding

Yuan

et al. 2020

J Exp Clin Cancer Res

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Background: Long intergenic non-protein coding RNA 520 (LINC00520), a novel identified lncRNA, has been shown to modulate the malignant phenotype of tumor cells in some malignant tumors. However, the exact role and molecular mechanism of LINC00520 in malignant melanoma has not been studied. Methods: The expression of LINC00520 in melanoma tissues were detected by using RNA-seq analysis and qRT-PCR. Melanoma cases from the public databases (The Cancer Genome Atlas (TCGA), GEO#GSE15605, GEO#GSE34460 and GEO#GSE24996) were included in this study. CCK-8 assay, EdU assay, transwell and scratch wound assay were used to explore the role of LINC00520 in melanoma cells. Luciferase reporter assays, MS2-RIP, RNA pull-down and RNA-ChIP assay were used to demonstrate the molecular biological mechanism of LINC00520 in melanoma. Results: We found that LICN00520 was found to be overexpressed in melanoma tissue. High expression of LICN00520 is a risk factor for the prognosis of melanoma patients. LINC00520 promotes the proliferation, invasion and migration of melanoma cells. LICN00520 exerted its oncogenic role by competitive binding miR-125b-5p to promote Eukaryotic initiation factor 5A2 (EIF5A2) expression. We also showed that LICN00520 promotes the growth and metastasis of melanoma in vivo through regulating miR-125b-5p/EIF5A2 axis. Conclusions: All results elucidated the role and molecular mechanism of LINC00520 in the malignant development of melanoma. LINC00520, a new oncogene in melanoma, maybe serve as a survival biomarkers or therapeutic target for melanoma patients.

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Expression profiling of long noncoding RNA identifies lnc‐MMP3‐1 as a prognostic biomarker in external auditory canal squamous cell carcinoma

Cited by 14 publications

References 29 publications

TUSC8 inhibits the development of osteosarcoma by sponging miR‑197‑3p and targeting EHD2

TUSC8 inhibits the development of osteosarcoma by sponging miR‑197‑3p and targeting EHD2

Long noncoding RNA LINC00518 acts as a competing endogenous RNA to promote the metastasis of malignant melanoma via miR-204-5p/AP1S2 axis

Long non-coding RNA LINC00520 promotes the proliferation and metastasis of malignant melanoma by inducing the miR-125b-5p/EIF5A2 axis

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