Expression profiling and bioinformatics analysis of circulating microRNAs in patients with acute myocardial infarction

Abstract

Objective Acute Myocardial Infarction (AMI) is the most severe type of coronary atherosclerotic heart diseases. MiRNA is a class of endogenous noncoding small molecule RNA, which plays an important regulatory role in the development of some diseases. Methods We examined the miRNA expression profiles in 16 patients with AMI compared with 6 non‐AMI controls using RNA sequencing. Results Compared with the miRNA expression profiles of non‐AMI controls, a total of 181 differentially expressed miRNAs were discriminated in AMI patients, of which 96 upregulated miRNAs and 85 downregulated miRNAs. The top ten upregulated miRNAs were as follows: miR‐449a‐5p, miR‐126‐5p, miR‐93‐5p, miR‐199a‐3p, miR‐4454, miR‐6880‐3p, miR‐3135a, miR‐548ad‐5p, miR‐4508, and miR‐556‐5p; while the top ten downregulated were as follows: miR‐6805‐5p, miR‐1228‐5p, miR‐939‐5p, miR‐615‐3p, miR‐6780a‐5p, miR‐6857‐3p, miR‐5088‐55p, miR‐7155‐3p, miR‐184, and miR‐4525. And the qRT‐PCR results of differentially expressed miRNAs showed the same result as high‐throughput sequencing data. For these 181 differentially expressed miRNAs, 19 841 target genes were predicted by GO analysis. The enrichment analysis revealed 2061 involved in biological processes, 353 in molecular function and 303 in cellular components. To identify biological pathways in AMI as compared to non‐AMI, the target genes of differentially expressed miRNAs were mapped to the classical signal transduction pathway in KEGG, indicating that 214 classes were enriched. ROC analysis showed that the circulating miRNAs had the important value for AMI diagnosis and supported the previous conclusions that circulating miRNAs were effective to diagnose the AMI as a novel biomarker. Conclusions Our findings require further research to confirm. It may provide a meaningful reference for the diagnosis and treatment of AMI.

A Novel miRNA Screen Identifies miRNA-4454 as a Candidate Biomarker for Ventricular Fibrosis in Patients with Hypertrophic Cardiomyopathy

“…The first two were previously described in the context of cardiac disease as mentioned above. Although the cardiac involvement of miR-4454 was suggested previously, since its plasma level was increased after myocardial infarction [ 40 ], our study is the first to identify miR-4454 as a biomarker for HCM.…”

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“…Furthermore, it was indicated that hsa-miR-4508 was a significant highly expressed miRNA in exosomes. It has been reported that circulating hsa-miR-4508 was significantly upregulated in acute myocardial infarction [ 37 ] and patients receiving dexmedetomidine [ 38 ]. However, its functions remain elusive.…”

Research on the circular RNA bioinformatics in patients with acute myocardial infarction

“…It has been shown to play a very important role in the development of various diseases, and the role of miRNAs in CVDs has also gradually been discovered. Studies have found that circulating miRNA‐134, miRNA‐22, miRNA‐328, and miRNA‐499 have abnormal expression levels in the plasma of patients with AMI, suggesting that they may be potential biomarkers of AMI 18‐21 . There are many miRNA response elements on circRNA.…”