Research on the circular RNA bioinformatics in patients with acute myocardial infarction

Abstract

Objective Through the detection of circular RNA (circRNA) using expression profiling chips, we searched for circRNAs related to acute myocardial infarction (AMI) and explored their relationship and possible mechanisms with AMI. Method The study subjects included 3 AMI patients and 3 controls, and circRNA expression profiling analysis was performed using a microarray gene chip to identify circRNAs with large differences in expression between groups and to construct a circRNA‐miRNA network. Results Compared with the control group, there were 650 differentially expressed circRNAs found in AMI patients (P < .05, fold change > 2), including 535 up‐regulated circRNAs, such as hsa_circ_0050908, hsa_circRNA4010‐22, hsa_circ_0081241, hsa_circ_0010551, hsa_circRNA4010‐20, hsa_circRNA14702, hsa_circ_0115392, has_circRNA1825‐44, has_circRNA8493‐7, and hsa_circ_0025097. Furthermore, there were 115 down‐regulated circRNAs, such as hsa_circ_0066439, hsa_circ_0054211, hsa_circ_0095920, hsa_circ_0122984, hsa_circ_0113067, hsa_circ_0039155, hsa_circRNA4014‐45, hsa_circ_0122979, hsa_circ_0059665, and hsa_circ_0009319. The circRNAs hsa_circ_0066439, hsa_circ_0081241, and hsa_circ_0122984 can regulate multiple signal pathways to participate in the AMI process through hsa‐miR‐1254, hsa‐miR‐328‐5p, and other miRNAs. In addition, the expression of circRNA‐miRNA in peripheral blood is related to the network. Differentially expressed circRNAs are involved in chromatin organization, chromatin‐modifying enzymes, signal transduction, lysine degradation, the mitogen‐activated protein kinase (MAPK) signaling pathway, focal adhesion, and a variety of other pathways, such as myocardial infarction, coronary heart disease, hypertension, and other diseases. The gene ontology analysis results show that molecular function mainly involves binding and molecular structural activity, whereas the biological process mainly involves a single biological process, a cellular component for organization, and a cellular process, and the cellular component mainly involves a protein complex, an extracellular matrix, and a membrane. Conclusion circRNA and microRNA interact to participate in the development of AMI. circRNA may be involved in the pathogenesis of AMI.

Exploring the mechanism of Yixinyin for myocardial infarction by weighted co-expression network and molecular docking

“…(Wuweizi) and Shell of Trichosanthes kirilowii (Gualoupi). Unlike the synthetic drugs, which generally produce their efficacy based on the theory of one drug and one target, the efficacy of TCMs relies on the comprehensive effects of multiple compounds, multiple targets, and multiple pathways 16 . Therefore, it is difficult to discover the key components and targets of Yixinyin in the treatment of MI from the molecular level.…”

“…In recent years, researchers have studied the pathogenesis of MI in order to find more available treatments 16 . Network pharmacology is a commonly-used technology for analyzing the mechanism of traditional Chinese medicine from the compound molecules and targets 17 .…”

Circular RNA hsa_circ_0000848 Regulates Cardiomyocyte Proliferation and Apoptosis Under Hypoxia via Recruiting ELAVL1 and Stabilizing SMAD7 mRNA

“…It has been commonly acknowledged that circRNAs are the key regulators in MI. 12 , 17 Hsa_circ_0000848 has been reported to be involved in trophoblast cell proliferation, migration, and apoptosis through interacting with miR-6768-5p. 18 Moreover, it has been reported that hsa_circ_0000848 can promote the cell proliferation of glioma by upregulating PCNA.…”

Emerging roles of circRNAs in the pathological process of myocardial infarction

“…Yin et al. 139 identified 650 circRNAs, including 535 upregulated circRNAs and 115 downregulated circRNAs, that were differentially expressed in AMI patients compared with control subjects. Myocardial expression of circRNAs is very high in AMI patients, and due to the stability and conserved nature of circRNAs, they are likely to be robust and sensitive biomarkers for MI.…”