Expression Profile of p63 in 127 Patients with Laryngeal Squamous Cell Carcinoma

Borba, Marcus Antônio de Mello; Cernea, Cláudio Roberto; Dias, Fernando Luiz; Faria, Paulo Antônio Silvestre de; Bacchi, Carlos E.; Brandão, Lenine Garcia; Costa, Ana Maria Dias da

doi:10.1159/000319904

Cited by 12 publications

(11 citation statements)

References 27 publications

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“…Additionally, the fractal dimension, which evaluates heterogeneity of tumour tissue and has been shown to be a prognostic factor in laryngeal carcinoma [ 15 ], was evaluated. Finally, the percentage of P63 expressed nuclei in the patient's digitized images was calculated, since it has been previously associated with patient prognosis [ 11 ].…”

Section: Methodsmentioning

confidence: 99%

“…However, grading of laryngeal lesions is influenced by the examining physician's experience and by low inter- and intraobserver reproducibility rates [ 8 – 10 ] amongst experienced pathologists. Regarding early diagnosis, many researchers have focused on identifying new prognostic factors, one such factor being the production of P63 protein by the TP63 gene, and the association of P63 overexpression with epithelial neoplasms of the lanynx [ 11 – 14 ]. It has been found [ 11 ] that in 96% of patients with laryngeal squamous cell carcinomas, the immunoexpression of P63 was present in over 30% of the cells, while in another study [ 14 ] the P63 cell-immunostaining cut-off was set at 50% and it was expressed in 95% of the patients with esophageal squamous cell carcinoma.…”

Section: Introductionmentioning

confidence: 99%

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Computer Based Correlation of the Texture of P63 Expressed Nuclei with Histological Tumour Grade, in Laryngeal Carcinomas

Νίνος

Kostopoulos

Kalatzis

et al. 2014

Analytical Cellular Pathology

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Background. P63 immunostaining has been considered as potential prognostic factor in laryngeal cancer. Considering that P63 is mainly nuclear stain, a possible correlation between the texture of P63-stained nuclei and the tumor's grade could be of value to diagnosis, since this may be related to biologic information imprinted as texture on P63 expressed nuclei. Objective. To investigate the association between P63 stained nuclei and histologic grade in laryngeal tumor lesions. Methods. Biopsy specimens from laryngeal tumour lesions of 55 patients diagnosed with laryngeal squamous cell carcinomas were immunohistochemically (IHC) stained for P63 expression. Four images were digitized from each patient's IHC specimens. P63 positively expressed nuclei were identified, the percentage of P63 expressed nuclei was computed, and 118 textural, morphological, shape, and architectural features were calculated from each one of the 55 laryngeal lesions. Data were split into the low grade (21 grade I lesions) and high grade (34 grade II and grade III lesions) classes for statistical analysis. Results. With advancing grade, P63 expression decreased, P63 stained nuclei appeared of lower image intensity, more inhomogeneous, of higher local contrast, contained smaller randomly distributed dissimilar structures and had irregular shape. Conclusion. P63 expressed nuclei contain important information related to histologic grade.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Computer Based Correlation of the Texture of P63 Expressed Nuclei with Histological Tumour Grade, in Laryngeal Carcinomas

Νίνος

Kostopoulos

Kalatzis

et al. 2014

Analytical Cellular Pathology

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“…Six features were computed from the two-level two-dimensional discrete wavelet transform (energy of level 1 and level 2 horizontal, vertical, and diagonal detail coefficients). One feature evaluated the percentage of p63-expressed nuclei in the patients' four digital images, since it has been claimed in a previous study [6,7] that p63 under-expression has been correlated with poor prognosis. Most of the above 71 features are functions readily available in Matlab.…”

Section: Feature Generationmentioning

confidence: 99%

“…Thus, researchers have focused on identifying new prognostic factors for laryngeal cancer. One such factor is the production of p63 protein by the TP63 gene and p63 over-expression in epithelial neoplasms of the head and neck squamous cell carcinomas [3][4][5][6][7]. p63 is a nuclear protein homolog of the tumor suppressor p53, involved in embryonic development, and is a marker of non-invasive epithelial tumors.…”

Section: Introductionmentioning

confidence: 99%

Microscopy image analysis of p63 immunohistochemically stained laryngeal cancer lesions for predicting patient 5-year survival

Νίνος

Kostopoulos

Kalatzis

et al. 2015

Eur Arch Otorhinolaryngol

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The aim of the present study was to design a microscopy image analysis (MIA) system for predicting the 5-year survival of patients with laryngeal squamous cell carcinoma, employing histopathology images of lesions, which had been immunohistochemically (IHC) stained for p63 expression. Biopsy materials from 42 patients, with verified laryngeal cancer and follow-up, were selected from the archives of the University Hospital of Patras, Greece. Twenty six patients had survived more than 5 years and 16 less than 5 years after the first diagnosis. Histopathology images were IHC stained for p63 expression. Images were first processed by a segmentation method for isolating the p63-expressed nuclei. Seventy-seven features were evaluated regarding texture, shape, and physical topology of nuclei, p63 staining, and patient-specific data. Those features, the probabilistic neural network classifier, the leave-one-out (LOO), and the bootstrap cross-validation methods, were used to design the MIA-system for assessing the 5-year survival of patients with laryngeal cancer. MIA-system accuracy was about 90 % and 85 %, employing the LOO and the Bootstrap methods, respectively. The image texture of p63-expressed nuclei appeared coarser and contained more edges in the 5-year non-survivor group. These differences were at a statistically significant level (p < 0.05). In conclusion, this study has proposed an MIA-system that may be of assistance to physicians, as a second opinion tool in assessing the 5-year survival of patients with laryngeal cancer, and it has revealed useful information regarding differences in nuclei texture between 5-year survivors and non-survivors.

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“…4 The expression of p63 was classified as typical or atypical. Typical expression was considered when it was similar to that observed in normal epithelium-that is, an intense nuclear staining in basal and suprabasal layers while no staining in the most superficial.…”

Section: Histopathologic and Immunohistochemistry Analysismentioning

confidence: 99%

p63 and E-cadherin Expression in Canine Oral Squamous Cell Carcinoma

Mestrinho¹,

Pissarra²,

Faísca

et al. 2014

Vet Pathol

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The expression of p63 and E-cadherin was studied in 22 oral squamous cell carcinomas in the dog according to immunohistochemical techniques. The association between these markers and clinicopathologic parameters was assessed. All tumor cells studied showed enhanced p63 expression. Regarding E-cadherin expression, 17 of 22 cases (77.3%) showed decreased immunoreactivity, and in 13 of 22 cases (59.1%), its expression was cytoplasmic. Neither p63 nor E-cadherin expression patterns were associated with tumor size, bone invasion, or lymph node metastasis. p63 score was related to proliferating cell nuclear antigen proliferative index (P ¼ .020). A statistically significant correlation between the expression patterns of these 2 markers was noted (P ¼ .026). Furthermore, they were related with tumor grade. An atypical p63 labeling and a cytoplasmic E-cadherin staining were statistically related with a higher tumor grade (P ¼ .022 and P ¼ .017, respectively). These findings suggest that changes in p63 and E-cadherin expression are frequent events in oral squamous cell carcinoma in dogs. Keywords p63, E-cadherin, oral squamous cell carcinoma, dogSquamous cell carcinoma (SCC) is the second-most frequent oral cancer in dogs 23 and one of the most common head and neck neoplasias in humans. 2,42 In spite of its low metastatic rate, this tumor is locally aggressive to the bone and other surrounding soft tissues. 23 Regardless of location, stage, and treatment approaches, local recurrence is very common, especially when clean surgical margins cannot be achieved.The role of p63 and E-cadherin expression in human head and neck SCC has been extensively studied, mainly with reference to their implications for prognosis. 4,12 However, there is a lack of understanding of the molecular pathogenesis underlying this cancer. 37 Substantial data indicate that both proteins play a relevant role in SCC initiation and progression. 16,29 Abnormal expression patterns of these 2 proteins have been associated with the decrease of epithelial characteristics of squamous cells and the acquisition of a mesenchymal-like phenotype.

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Expression Profile of p63 in 127 Patients with Laryngeal Squamous Cell Carcinoma

Cited by 12 publications

References 27 publications

Computer Based Correlation of the Texture of P63 Expressed Nuclei with Histological Tumour Grade, in Laryngeal Carcinomas

Computer Based Correlation of the Texture of P63 Expressed Nuclei with Histological Tumour Grade, in Laryngeal Carcinomas

Microscopy image analysis of p63 immunohistochemically stained laryngeal cancer lesions for predicting patient 5-year survival

p63 and E-cadherin Expression in Canine Oral Squamous Cell Carcinoma

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